A5053851350- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant and animal studies
- Seed Germination and Physiology
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Transgenic Plants and Applications
- Agronomic Practices and Intercropping Systems
- Phosphorus and nutrient management
- Genetics, Aging, and Longevity in Model Organisms
- Soybean genetics and cultivation
- Bioinformatics and Genomic Networks
- Clay minerals and soil interactions
- Plant Pathogens and Resistance
- Plant biochemistry and biosynthesis
- Single-cell and spatial transcriptomics
- Plant Diversity and Evolution
- Diet and metabolism studies
- RNA modifications and cancer
- Bioenergy crop production and management
- Ocular Disorders and Treatments
- Multisensory perception and integration
- Turfgrass Adaptation and Management
- Plant Pathogens and Fungal Diseases
- Fern and Epiphyte Biology
Wellcome Centre for Cell-Matrix Research
2021-2025
University of Manchester
2021-2025
Manchester Academic Health Science Centre
2021-2025
Aberystwyth University
2024
Genomics England
2020-2021
The University of Adelaide
1998
Commonwealth Scientific and Industrial Research Organisation
1998
University of Georgia
1990-1992
University of Kentucky
1986-1991
The University of Melbourne
1984-1988
Basement membranes (BMs) are ubiquitous extracellular matrices whose composition remains elusive, limiting our understanding of BM regulation and function. By developing a bioinformatic in vivo discovery pipeline, we define network 222 human proteins their animal orthologs localized to BMs. Network analysis screening
When immature zygotic embryos of Trifolium repens are cultured in vitro the presence 0.05 mg 1−1 BAP, cells hypocotyl epidermis proliferate to produce somatic embryoids directly without an intervening callus phase. The young epidermal show features proembryogenic cells, and first sign embyroid induction is a shift from regular equational, anticlinal divisions irregular, periclinal oblique quantal divisions. Multicellular budding single-cell initiation apparently both occur, with...
Ant pollination systems are remarkably rare. We show that pollen exposed to ants for brief periods exhibits reduced viability, percent germination, and shorter tubes relative control pollen. Pollination with ant-borne also results in lower seed-set than untreated This disruption of processes must have exerted a powerful selection pressure against the evolution ofant-pollination systems. It is suggested nestbuilding brood-rearing habits require they secrete large amounts antibiotics combat...
The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into network within BMs. Endogenous tagging all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding this crucial BM component. Through genome editing, we show the C termini C. elegans EMB-9 and LET-2 can be fused to variety create strain toolkit wild-type health. Using quantitative imaging, results suggest preference for...
Alport syndrome is a rare genetic disorder characterized by progressive kidney disease, hearing loss, and eye abnormalities. Gene therapy for has not yet been realized due to technical challenges, including effective transduction of target cells. In this study, we established quantitative testing platform using podocytes in culture, ex vivo glomeruli, mouse model evaluate the efficacy adeno-associated Virus-9 (AAV9) as gene delivery vehicle. We compared levels AAV9-GFP vectors between...
Abstract The implications of the quantity and intensity aspects soil P status have been studied over a range 4° contrasting acid soils by examining (a) interrelationships (b) correlations with yield uptake oats from pot cultures receiving no added P, for (I) so‐called L value, (2) readily‐soluble (P x ) extracted six conventional methods, (3) total (solid + liquid phase) q involved in these extractions. representing isotopically exchangeable sampled plants cultures, value were estimated aid...
In Rhododendron spp. and Ledum groenlandicum a callose wall is laid down around the zygote in first 2 days after fertilization. The periodic acid/Schiff-positive, aniline blue-fluorescence-positive callosic initiated adjacent to degenerating synergid, extends cover entire surface, remains visible during initiation of embryogeny as elongates before proembryonal division. Unfertilized ovules show eventual deposition ovule cells senescence undeveloped abscising pistils, but no development...
This research reports a new technique for chromosome doubling of clover (Trifolium sp.) axillary meristems via in vitro colchicine application. Plant material utilized included T. pratense (red clover) cv. Kenstar clones, and three interspecific hybrids: ambiguum (kura × repens (white clover); alpestre pratense; sarosiense pratense. Vegetative were excised from plants, surface sterilized, trimmed to length 0.5–1 mm. Meristems placed on the shoot proliferation medium (ML8) containing (0.1%)...
Early events in the embryo sac of Rhododendron kawakamii and R. retusum have been studied after compatible self-pollinations eight interspecific crosses, using sectioned ovaries, pistil squashes, seed-set data. Ovules are anatropous, unitegmic, tenuinucellate, with a typical eight-nucleate, seven-celled sac. Fertilization normally occurs 4–5 days pollination. The zygote lays down callose wall but remains undivided during first 13–15 primary endosperm nucleus divides soon fertilization,...
A total of 55 regenerants from direct somatic embryogenesis on three zygotic embryos Trifolium repens have been examined for uniformity with respect to morphological markers, chromosome number, breeding behaviour and banding patterns produced by SDS-polyacrylamide gel electrophoresis leaf proteins isoelectric focussing peroxidase, esterase leucine aminopeptidase isozymes. Differences were observed between clones, but no differences detected among primary secondary the same embryo. The data...
Kidney physiology shows diurnal variation, and a disrupted circadian rhythm is associated with kidney disease. However, it remains largely unknown whether glomeruli, the filtering units in kidney, are under control. Here, we investigated core clock components together their rhythmic targets modes of regulation. With gene reporter mice, cell-autonomous glomerular clocks which likely govern fluctuations were identified. Using time-series transcriptomic profiling, first transcriptome 375...