A5087641663- Zebrafish Biomedical Research Applications
- Neurobiology and Insect Physiology Research
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Advanced Memory and Neural Computing
- Neuroscience of respiration and sleep
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Retinal Development and Disorders
- Bacterial biofilms and quorum sensing
- Photoreceptor and optogenetics research
- MicroRNA in disease regulation
- Microbial Metabolism and Applications
- Mechanisms of cancer metastasis
- Probiotics and Fermented Foods
- Physiological and biochemical adaptations
- Milk Quality and Mastitis in Dairy Cows
- Escherichia coli research studies
- Antimicrobial Peptides and Activities
- RNA modifications and cancer
- Congenital heart defects research
- Cerebrospinal fluid and hydrocephalus
The First Affiliated Hospital, Sun Yat-sen University
2021-2024
Tianjin Institute of Pharmaceutical Research (China)
2021-2024
University of St Andrews
2015-2024
Hainan University
2024
Sun Yat-sen University
2021-2024
Key Laboratory of Guangdong Province
2024
Anhui Agricultural University
2016-2023
Fudan University
2023
Zhongshan Hospital
2023
Tianjin Medical University
2022
Adult nervous systems are so complex that understanding how they produce behavior remains a real challenge. We chose to study hatchling Xenopus tadpoles where is controlled by few thousand neurons but there very limited number of types neuron. Young can flex, swim away, adjust their trajectory, speed-up and slow-down, stop when contact support struggle grasped. They sensitive touch, pressure, noxious stimuli, light intensity water currents. Using whole-cell recording has led rapid progress...
Abstract Alzheimer’s disease (AD) is a progressive neurodegenerative with high prevalence rate among the elderly population. A large number of clinical studies have suggested repetitive transcranial magnetic stimulation (rTMS) as promising non-invasive treatment for patients mild to moderate AD. However, underlying cellular and molecular mechanisms remain largely uninvestigated. In current study, we examined effect frequency rTMS on cognitive functions pathological changes in brains 4-...
The ability of brief stimuli to trigger prolonged neuronal activity is a fundamental requirement in nervous systems, common motor responses and short-term memory. Bistable membrane properties network feedback excitation have both been proposed as suitable mechanisms sustain such persistent responses. There now good experimental evidence for bistability. In contrast, the long-standing hypotheses based on positive yet be supported by direct mutual excitatory connections between appropriate...
Rhythmic activity is central to brain function. In the vertebrate CNS, neuronal circuits for breathing and locomotion involve inhibition also neurons acting as pacemakers, but identifying responsible has proven difficult. By studying simple hatchling Xenopus laevis tadpoles, we have already identified a population of electrically coupled hindbrain (dINs) that drive swimming. During rhythm generation, dINs release glutamate excite each other activate NMDA receptors (NMDARs). The resulting...
Major depressive disorder is one of the most common mental health conditions. Meningeal lymphatics are essential for drainage molecules in cerebrospinal fluid to peripheral immune system. Their potential role depression-like behaviour has not been investigated. Here, we show mice, sub-chronic variable stress as a model impairs meningeal females but males. Manipulations regulate sex difference susceptibility stress-induced depression- and anxiety-like behaviors well alterations medial...
Rheumatoid arthritis (RA) patients have a high prevalence for depression. On the other hand, comorbid with depression is associated worse prognosis RA. However, little known about underlying mechanisms comorbidity between RA and It remains to be elucidated which brain region critically involved in development of RA, whether alterations may affect pathological symptoms. Here, by combining clinical animal model studies, we show that patients, level significantly correlated severity disease...
Understanding the neuronal networks in mammal spinal cord is hampered by diversity of neurons and their connections. The simpler developing lower vertebrates may offer insights into basic organization. To investigate function inhibitory interneurons Xenopus tadpoles, paired whole-cell recordings were used. We show directly that one class interneuron, with distinctive anatomy, produces glycinergic, negative feedback inhibition can limit firing motoneurons central pattern generator during...
Motor networks typically generate several related output patterns or gaits where individual neurons may be shared recruited between patterns. We investigate how a vertebrate locomotor network is reconfigured to produce second rhythmic motor pattern, defining the detailed pattern of neuronal recruitment and consequent changes in mechanism for rhythm generation. Hatchling Xenopus tadpoles swim if touched, but when held make slower, stronger, struggling movements. In immobilized tadpoles, brief...
Important questions remain about the origin of excitation that drives locomotion in vertebrates and roles played by reticulospinal neurons. In young Xenopus tadpoles, paired whole-cell recordings reveal neurons directly excite swimming circuit brainstem spinal cord. They form part a column (dINs) with ipsilateral descending projections which fire reliably rhythmically time swimming. We ask if, at this early stage development, these are themselves primary source rhythmic drive to cord on each...
Electrical coupling is important in rhythm generating systems. We examine its role circuits controlling locomotion a simple vertebrate model, the young Xenopus tadpole, where hindbrain and spinal cord excitatory descending interneurons (dINs) that drive maintain swimming have been characterised. Using simultaneous paired recordings, we show most dINs are electrically coupled exclusively to other (DC coefficients approximately 8.5%). The shows typical low-pass filtering. found no evidence...
Alternating contractions of antagonistic muscle groups during locomotion are generated by spinal "half-center" networks coupled in antiphase reciprocal inhibition. It is widely thought that inhibition only coordinates the activity these muscles. We have devised two methods to rapidly and selectively silence neurons on just one side Xenopus tadpole cord hindbrain, which generate swimming rhythms. Silencing led rapid cessation other side. Analyses reveal this resulted from depression...
Most neurons release a single fast-acting low-molecular-weight transmitter at synapses to activate and open postsynaptic ion channels. We challenge this principle with evidence for corelease of the two major excitatory transmitters, glutamate acetylcholine (ACh), from identified in developing frog tadpole spinal cord. Whole-cell patch electrodes were used record neurons. When action potentials inhibition blocked, spontaneous miniature currents (mEPSCs) recorded. These fully blocked only by...
High-impedance, sharp intracellular electrodes were compared with whole cell patch by recording from single spinal neurons in immobilized frog tadpoles. A range of neuron properties examined using or test while making simultaneous recordings a second control electrode. Overall, did not significantly alter the activity recorded electrode, and two essentially identical. In contrast, electrode differed initial recordings. some cases, differences due to real changes properties: resting membrane...
The Chinese Chang'e 5 mission has returned the youngest mare basalts (∼2.0 Ga) identified thus far. However, their mantle source remains highly debated. Here, we present an integrated study of mineralogy, chemical composition, Pb–Pb age, and whole rock Sr–Nd–Hf isotopic compositions one (CE5) basalt clast. indistinguishable age (1.96 ± 0.09 Ga), similar mineral Sr–Nd to those previously reported CE5 indicate that they had a common source. After correction for cosmic ray effects, εNd-εHf...
Abstract Background How specific are the synaptic connections formed as neuronal networks develop and can simple rules account for formation of functioning circuits? These questions assessed in spinal circuits controlling swimming hatchling frog tadpoles. This is possible because detailed information now available on identity main types neuron. Results The probabilities synapses between 7 identified neuron were measured directly by making electrical recordings from 500 pairs neurons. For...
Every type of neural rhythm has its own operational range frequency. Neuronal mechanisms underlying rhythms at different frequencies, however, are poorly understood. We use a simple aquatic vertebrate, the two-day-old Xenopus tadpole, to investigate how brainstem and spinal circuits generate swimming speeds. first determined that basic motor output pattern was not altered with varying frequencies. The firing reliability types rhythmic neuron involved in then analyzed. results showed there...
Currently, mechanical and chemical damage is the main way to carry out weed control. The use of chlorophyll fluorescence (CF) technology nondestructively monitor stress physiological state weeds significant reveal mechanism stresses as well complex stresses. Under simulated real field environmental conditions, different species leaf age (Digitaria sanguinalis 2-5 age, Erigeron canadensis 5-10 age) were subjected experimental treatments for 1-7 days, parameters measured every 24 h using a...
Neurobiotin was injected into individual spinal interneurons in the Xenopus tadpole to discern their anatomical features and complete axonal projection patterns. Four classes of interneuron are described, with names defining primary axon projection: Dorsolateral ascending commissural predominantly multipolar cells somata dendrites exclusively dorsal half cord. Ascending have unipolar located half, but main ventral Descending show bigger variance anatomy, majority unipolar, they all a...
During locomotion, reflex responses to sensory stimulation are usually modulated and may even be reversed. This is thought the result of phased inhibition, but neurons responsible not known. When hatchling Xenopus tadpole swims, cutaneous modulated. occurs because pathway interneurons receive rhythmic glycinergic inhibition broadly in phase with motor discharge on same side trunk. We now describe a new whole-cell recording preparation CNS. has been used neurobiotin injection define passive...
Recent recordings from spinal neurons in hatchling frog tadpoles allow their type-specific properties to be defined. Seven main types of neuron involved the control swimming have been characterized. To investigate significance properties, we build models each type and assemble them into a network using known connectivity between: sensory neurons, pathway interneurons, central pattern generator (CPG) interneurons motoneurons. A single stimulus initiates where modelled neuronal activity...
Abstract Activity-dependent modification of neural network output usually results from changes in neurotransmitter release and/or membrane conductance. In Xenopus frog tadpoles, spinal locomotor is adapted by an ultraslow afterhyperpolarization (usAHP) mediated increase Na + pump current. Here we systematically explore how the interval between two swimming episodes affects second episode, which shorter and slower than first episode. We find firing reliability rhythmic neurons to be lower...
Unlike the monosynaptic "stretch" reflex, exact neuronal pathway for a simple cutaneous reflex has not yet been defined in any vertebrate. In young frog tadpoles, we made whole-cell recordings from pairs of spinal neurons. We found direct, excitatory, glutamatergic synapses touch-sensitive skin-sensory neurons to sensory interneurons, and then these interneurons motoneurons premotor on other side body. conclude that minimal this primitive which stroking skin one leads flexion side, is...