A5006162285- Influenza Virus Research Studies
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- Hepatitis C virus research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- COVID-19 diagnosis using AI
- COVID-19 epidemiological studies
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Viral Infections and Vectors
Institut Pasteur
2005-2023
Centre National de la Recherche Scientifique
1999-2021
Université Paris Cité
2007-2021
Centre de Gestion Scientifique
2021
Department of Virology
2020
Délégation Paris 7
2013
Sorbonne Paris Cité
2013
Different serological assays measuring anti–SARS-CoV-2 antibodies and their neutralizing activity in samples from individuals with severe mild COVID-19 are compared.
Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly budding enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from endoplasmic reticulum-Golgi intermediate compartment. In this study, distribution maturation SARS-CoV surface S, M E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into reticulum, high-mannosylated S...
Background Children’s role in SARS-CoV-2 epidemiology remains unclear. We investigated an initially unnoticed outbreak linked to schools northern France, beginning as early mid-January 2020. Aims This retrospective observational study documents the extent of transmission, affected high school (n = 664 participants) and primary 1,340 participants), context unsuspected circulation limited control measures. Methods Between 30 March April 2020, all staff, well pupils their parents relatives were...
In order to determine how efficiently the polymerase proteins derived from human and avian influenza A viruses can interact with each other in context of a mammalian cell, genetic system that allows vivo reconstitution active ribonucleoproteins was used. The ability achieve replication viral-like reporter RNA COS-1 cells examined heterospecific mixtures core (PB1, PB2, PA NP) two strains (A/Puerto Rico/8/34 A/Victoria/3/75), (A/Mallard/NY/6750/78 A/FPV/-Rostock/34), strain origin (A/Hong...
Like most RNA viruses, influenza viruses generate defective viral genomes (DVGs) with large internal deletions during replication. There is accumulating evidence supporting a biological relevance of such DVGs. However, further understanding the molecular mechanisms that underlie production and activity DVGs conditioned upon sensitivity accuracy detection methods, is, next-generation sequencing (NGS) technologies related bioinformatics algorithms. Although many algorithms were developed,...
Significant percentages of patients suffering from non-A non-B hepatitis (43%) and B (35%) were found to release an Ig-binding factor in their stools. This factor, which we called "protein F" was less frequently observed (20%) other liver disorders, only 6.7% healthy subjects (p than 10(-7), 10(-4), 0.03, respectively). The specificity the detection test (a nonimmune ELISA-like assay) confirmed by inhibition experiments. Binding located on F(ab) fragment Ig, irrespectively isotype. Protein F...
ABSTRACT The rescue of influenza viruses by reverse genetics has been described only for the A and B viruses. Based on a similar approach, we developed reverse-genetics system that allows production C entirely from cloned cDNA. complete sequences 3′ 5′ noncoding regions type virus C/Johannesburg/1/66 necessary cloning cDNA were determined seven genomic segments. Human embryonic kidney cells (293T) transfected simultaneously with plasmids direct synthesis each viral RNA segments C/JHB/1/66...
During the last decade, role of wildlife in emerging pathogen transmission to domestic animals has often been pointed out. Conversely, far less attention paid from wildlife. Here, we focus on case game restocking, which implies release millions worldwide each year. We conducted a 2-year study Camargue (Southern France) investigate influence hand-reared Mallard releases avian influenza virus dynamics surrounding sampled Mallards (cloacal swabs) several duck facilities 2009 and 2010 before...
The transcription/replication of the influenza viruses implicate terminal nucleotide sequences viral RNA, which comprise at extremities conserved among genomic segments as well variable 3' and 5' non-coding (NC) regions. plasmid-based system for in vivo reconstitution functional ribonucleoproteins, upon expression viral-like RNAs together with nucleoprotein polymerase proteins has been widely used to analyze viruses. It was thus shown that type A could transcribe replicate A, B, or C vRNA...
The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal upper airways, site of initial replication.The IgG and IgA were analysed plasma nasopharyngeal swabs from first four confirmed France. Two pauci-symptomatic while two developed severe disease. We characterized their profiles by using an in-house ELISA detect antibodies directed against Nucleoprotein Spike.Anti-N detected NPS only. levels markedly differed...
We have analyzed the structures, relative organization, and activities of binding sites for nuclear factors in duck hepatitis B virus (duck HBV) enhancer. DNase I footprinting analysis mobility shift assays demonstrate that this enhancer 192 bp contains at least three transcription factors: one hepatocyte-adipocyte C/EBP, a second liver-specific transactivator hepatocyte factor 1 HNF-1, third factor, called F3, which binds to DNA sequence bearing some resemblance ubiquitous EF-C. Analysis...
The genome of the duck hepatitis B virus (DHBV) contains an enhancer element. This sequence, 192 bp, is located in 3'-terminal coding region DNA polymerase gene (nucleotides 2159 to 2351), upstream from pregenomic RNA start site. potentiates a marked increased activity heterologous thymidine kinase promoter orientation-independent manner and at proximal, as well distal, location. DHBV activates transcription relatively cell-type-independent manner. Sequence homologies with nuclear factor...
Exchange of the extremities NS segment type A and C influenza viruses in reverse genetics systems was used to assess their putative role specificity. Restoration each specific proximal panhandle mandatory allow rescue with heterotypic extremities. Moreover, transcription level modified seemed be directly affected by distal strength.
Influenza A and C viruses share common sequences in the terminal noncoding regions of viral RNA segments. Differences at 5′- 3′-ends exist, however, that could contribute to specificity with which transcription/replication signals are recognized by cognate polymerase complexes. Previously, making use a transient expression system for transcription replication reporter template bearing either type or extremities, it was shown is transcribed replicated equal efficiency complex, whereas less...
The seroprevalence of SARS-CoV-2 infection in the French population was estimated with a representative, repeated cross-sectional survey based on residual sera from routine blood testing. These data contained no information or vaccination status, thus limiting ability to detail changes observed immunity level over time.Our aim is predict infected vaccinated status individuals serosurveillance only results serological assays. Reference longitudinal profiles seronegative, infected, and another...
Exchange of the non coding regions NP segment between type A and C influenza viruses was used to demonstrate importance not only proximal panhandle, but also initial distal panhandle strength in specificity. Both elements were found be compulsory rescue infectious virus by reverse genetics systems. Interestingly, context, length 5′ NC region once transcribed into mRNA impact its translation, level produced protein consequently affected viral genome replication.
Abstract For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter adjacent parts of are involved in packaging, but precise role NC still unclear. aim this study to better understand incorporation viral segments into virions. NA-segment were systematically replaced those seven other segments. Recombinant viruses harbouring two with identical successfully rescued....
Abstract Background The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, much less is known about the mucosal upper airways at site of initial replication. Local nasopharyngeal epithelium, that are likely determine course infection, have not analysed so far nor their correlation with serum. Methods IgG and IgA were plasma as well swabs (NPS) from first four confirmed by RT-qPCR France. Two pauci-symptomatic while two developed severe...
<sec> <title>BACKGROUND</title> The seroprevalence of SARS-CoV-2 infection in the French population was estimated with a representative, repeated cross-sectional survey based on residual sera from routine blood testing. These data contained no information or vaccination status, thus limiting ability to detail changes observed immunity level over time. </sec> <title>OBJECTIVE</title> Our aim is predict infected vaccinated status individuals serosurveillance only results serological assays....