A5025163258- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Coronary Interventions and Diagnostics
- Cardiac Imaging and Diagnostics
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Protein purification and stability
- Cerebrovascular and Carotid Artery Diseases
- SARS-CoV-2 detection and testing
- Acute Myocardial Infarction Research
- Viral-associated cancers and disorders
- Synthesis and biological activity
- Metal Forming Simulation Techniques
- Microstructure and Mechanical Properties of Steels
- Metallurgy and Material Forming
- Vascular Procedures and Complications
- Bacterial Infections and Vaccines
- Angiogenesis and VEGF in Cancer
- Cardiac and Coronary Surgery Techniques
- Nonprofit Sector and Volunteering
- Peripheral Artery Disease Management
University of California, San Diego
2010-2023
Allen Institute
2023
Dana-Farber Cancer Institute
2020-2023
Biogen (United States)
2022
Dignity Health
2022
University of Pittsburgh
2009
We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlororaphis phage 201φ2-1 assembled compartment separated DNA from cytoplasm. The was centered by bipolar tubulin-based spindle, it segregated bacterial proteins according to function. Proteins involved replication transcription localized inside compartment, whereas translation nucleotide synthesis outside. Later...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected over 21 million people worldwide since August 16, 2020. Compared to PCR and serology tests, SARS-CoV-2 antigen assays are underdeveloped, despite their potential identify active infection monitor disease progression. Methods We used Single Molecule Array (Simoa) quantitatively detect spike, S1 subunit, nucleocapsid antigens in the plasma of patients with (COVID-19). studied from 64 who were COVID-19...
We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201φ2-1 assembles a nucleus-like structure encloses phage DNA and segregates proteins according to function, with processing inside metabolic enzymes ribosomes outside nucleus. Here, we investigate replication pathway of aeruginosa bacteriophages φKZ φPA3. Bacteriophages φPA3 encode proteinaceous shell compartmentalizes during viral infection. show tubulin-like protein PhuZ encoded by each bipolar spindle displays...
Despite being essential for successful infection, the molecular cues involved in host recognition and genome transfer of viruses are not completely understood. Bacterial outer membrane proteins A C co-purify lipid vesicles with bacteriophage Sf6, implicating both as potential receptors. We determined that mediate Sf6 infection by dramatically increasing its rate efficiency. performed a combination vivo studies three omp null mutants Shigella flexneri, including classic phage plaque assays...
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to randomly throughout the host cell cytoplasm. Here, we show that viral of
Abstract In the arms race between bacteria and bacteriophages (phages), some large-genome jumbo phages have evolved a protein shell that encloses their replicating genome to protect it against host immune factors. By segregating from cytoplasm, however, ‘phage nucleus’ introduces need specifically translocate messenger RNA proteins through nuclear dock capsids on for packaging. Here, we use proximity labeling localization mapping systematically identify associated with major chimallin (ChmA)...
Cyanoacetic acid derivatives are the starting materials for a plethora of multicomponent reaction (MCR) scaffolds. However diversity these scaffolds is hampered by low variability cyanoacetic in past. Here we describe valuable protocols parallel synthesis arrays cyanoacetamides on multigram scale and involving very convenient work up simple filtration washing. Fifty-two products described, several applications indicated. We foresee our protocol resulting to become greatly expanding large MCR...
Abstract Understanding how biological species arise is critical for understanding the evolution of life on Earth. Bioinformatic analyses have recently revealed that viruses, like multicellular life, form reproductively isolated species. Viruses are known to share high rates genetic exchange, so do they evolve isolation? Here, we evaluate two related bacteriophages and describe three factors limit exchange between them: 1) A nucleus-like compartment physically separates replicating phage...
Upon infection of Pseudomonas cells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous shell along with proteins associated replication, recombination transcription. Ribosomes involved in metabolic processes are excluded from RNA synthesis occurs inside nucleus messenger presumably transported into cytoplasm to be translated. Newly synthesized either remain or specifically translocate The molecular mechanisms governing...
Monoclonal antibodies are a promising approach to treat COVID-19, however the emergence of SARS-CoV-2 variants has challenged efficacy and future these therapies. Antibody cocktails being employed mitigate challenges, but neutralization escape remains major challenge alternative strategies needed. Here we present two anti-SARS-CoV-2 spike binding antibodies, one Class 1 4, selected from our non-immune human single-chain variable fragment (scFv) phage library, that engineered into four,...
Background: Wall shear stress (SS) plays an important role in the initiation and proliferation of coronary atherosclerosis, especially for bifurcations. Stenting artery will cause many different changes velocity, flow, cross‐sectional area, wall SS. However, it is still unknown how much SS distribution varies with stenting bifurcation. Objective: The purpose this study was to investigate magnitude after classical crush bifurcation lesions. Methods: Eleven patients true by technique were...
Abstract When sensory information is incomplete or ambiguous, the brain relies on prior expectations to infer perceptual objects. Despite centrality of this process perception, neural mechanism inference not known. Illusory contours (ICs) are key tools study because they contain edges objects that implied only by their spatial context. Using cellular resolution, mesoscale two-photon calcium imaging and multi-Neuropixels recordings in mouse visual cortex, we identified a sparse subset neurons...
In the arms race between bacteria and bacteriophages (phages), some large-genome jumbo phages have evolved a protein shell that encloses their replicating genome to protect it against DNA-targeting immune factors. By segregating from host cytoplasm, however, "phage nucleus" introduces need specifically transport mRNA proteins through nuclear shell, dock capsids on for packaging. Here, we use proximity labeling localization mapping systematically identify associated with major chimallin...
Upper gastrointestinal (UGI) bleeding is an uncommon manifestation of acquired immune deficiency syndrome (AIDS) despite frequent involvement the tract by infections, malignancies, and other disorders (1). Primary gastric Burkitt lymphoma rare in children; only 4 pediatric cases lymphomas have been reported previously (2–4). According to our review literature, this first case report a child with human immunodeficiency virus (HIV)/AIDS presenting upper due lymphoma. An 11-year-old African...
Endothelial shear stress is one of the local hemodynamic factors suspected in development coronary atherosclerosis bifurcation lesions. In patients with provisional stenting, endothelial (SS) distribution unknown.The aim this study was to investigate magnitude and SS lesions stenting by approach.Ten consecutive were included study. Quantitative analysis, flow study, three-dimensional computational analysis aid commercial software CD STAR-CCM+ done before after procedure also 8 months...
Abstract Upon infection of Pseudomonas cells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous shell along with proteins associated replication, recombination transcription. Ribosomes involved in metabolic processes are excluded from RNA synthesis occurs inside nucleus messenger presumably transported into cytoplasm to be translated. Newly synthesized either remain or specifically translocate The molecular mechanisms...
Methamphetamine intoxication is a known risk factor for nonocclusive mesenteric ischemia (NOMI). We describe case of 50-year-old male with history polysubstance abuse who presented to the Emergency Department severe abdominal pain and coffee-ground emesis. Computed tomographic (CT) imaging demonstrated portal venous gas diffuse colonic wall thickening concerning ischemic colitis. The patient underwent exploratory laparotomy resection ascending colon as well necrotic section jejunum. Further...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people worldwide. PCR tests are currently the gold standard for diagnosis current disease (COVID-19) and serology used to detect seroconversion in patients. However, there is a lack quantitative ultra-sensitive viral antigen COVID-19. Here we show that Single Molecule Array (Simoa) assays can quantitatively SARS-CoV-2 spike, S1 subunit, nucleocapsid antigens plasma COVID-19 Combined with Simoa...
Summary Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages were long assumed to randomly throughout the host cell cytoplasm. Here we visualized location of viral formed during replication Pseudomonas nucleus-forming jumbo phages and discovered that they a unique structure inside cells term phage bouquets. We show after capsids complete DNA packaging at surface nucleus, tails attach capsids, these accumulate form bouquets specific...