A5023261613- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Virology and Viral Diseases
- T-cell and Retrovirus Studies
- Viral Infections and Vectors
- Microbial infections and disease research
- Bird parasitology and diseases
- Science and Education Research
- Animal Nutrition and Physiology
- Effects of Environmental Stressors on Livestock
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 and COVID-19 Research
- Livestock and Poultry Management
- Plant and animal studies
- Molecular Biology Techniques and Applications
- interferon and immune responses
- Insect Resistance and Genetics
Concordia University Wisconsin
2004-2018
Brazilian Agricultural Research Corporation
1993-2013
Agricultural Research Corporation
2012
Universidade Estadual Paulista (Unesp)
2008
North Carolina State University
1998
Empresa de Pesquisa Agropecuária do Rio Grande do Norte
1995
Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract chickens, inducing both morphological and immune modulatory changes. However, association between local responses induced by IBV mechanisms pathogenesis has not yet been completely elucidated. This study compared expression profile genes related to tracheal samples after challenge with two Brazilian field isolates (A B) from same genotype, associating these viral replication...
A infecção causada pelo vírus Influenza (IAV) é endêmica em suínos no mundo inteiro. O surgimento da pandemia de influenza humana A/H1N1 (pH1N1) 2009 levantou dúvidas sobre a ocorrência deste Brasil. Durante o desenvolvimento um projeto pesquisa do suína 2009-2010, na Embrapa Suínos e Aves (CNPSA), foi detectado rebanho Santa Catarina, Brasil, surto altamente transmissível causado subtipo viral H1N1. Este causou uma doença leve crescimento fêmeas adultas, sem mortalidade. Tres leitões...
ABSTRACT The reovirus M1, L1, and L2 genes encode proteins found at each vertex of the viral core are likely to form a structural unit involved in RNA synthesis. Genetic analyses have implicated M1 gene synthesis nucleoside triphosphatase activity, but there been no direct biochemical studies μ2 function. Here, we expressed vitro assessed its RNA-binding activity. binds both poly(I-C)- poly(U)-Sepharose, binding activity is greater Mn 2+ than Mg . Heterologous competes for transcripts as...
Twelve Brazilian isolates and one reference vaccine strain of avian infectious bronchitis virus (IBV) were propagated in embryonating chicken eggs. The entire S1 glycoprotein gene these viruses was analysed by reverse-transcriptase-polymerase chain reaction restriction fragment length polymorphism (RT-PCR-RFLP), using the enzymes HaeIII, XcmI BstyI. RFLP patterns led to classification into five distinct genotypes: A, B, C, D Massachusetts. Five twelve grouped Massachusetts genotype remaining...
Seven chicken infectious anemia virus (CIAV) isolates were obtained from seven broiler flocks with poor performance in two states of Brazil. All induced thymus atrophy, bone-marrow aplasia, and low hematocrit values when inoculated into 1-day-old susceptible chicks. The CIAV resistant to treatment chloroform able pass through 50-nm-pore-size filters. CIAV-specific antigens could be demonstrated tissues experimentally infected chicks using a monoclonal antibody specific for CIAV. These...
A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains discriminate H120 vaccine strain from other strains.Viral RNA extracted allantoic fluid chicken embryos tissues chickens experimentally infected with different IBV.Amplification identification viral performed using two sets primers complementary a region in Semi-N-RT-PCR assay.The pair first PCR consisted universal...
Apesar do diagnóstico da anemia infecciosa e mais evidências recentes de infecção em campo (Brentano & Ottati, 1999), não havia até hoje dados disponíveis prevalência vírus das galinhas (CAV) no Brasil. Uma vez que o conhecimento distribuição anticorpos nos lotes matrizes é essencial para delineamento estratégias controle doença, realizamos um levantamento 5 Estados com produção comercial intensiva linhas corte, abrangendo aves diferentes idades. De 2.355 soros testados por ELISA, 89%...
A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region VP1 gene, diagnosis CAV in organ samples broiler flocks suspected chicken infectious anemia. comparison VP3/VP1 with vivo virus isolation revealed 100% agreement results, 13 positive 3 negative both assays, indicating that is specific diagnostic method. Tissues from additional 24 flocks, CAV-like lesions clinical history were then tested only by reference published primers gene. Nineteen resulted one...
Rapid, sensitive and specific methods are necessary to confirm the diagnosis of outbreaks avian infectious bronchitis virus (IBV) infection. The amplification IBV genome by reverse transcription followed polymerase chain reaction (RT-PCR) has been one most used for detection this in clinical samples. To reduce time number steps molecular IBV, we developed a rapid method based on viral capture lectin (Concanavalin A—Con A) microplate wells, RT-PCR amplify S1 gene. limit was 103 EID50/ml...