The aspects involved in broiler water intake are not well known, despite the importance of animal nutrition and physiology. Water behavior should be taken into account when deciding on different types drinkers. Bell nipple drinkers most commonly used commercial production. Broilers were housed cages equipped with two drinker raised at environmental temperatures (25 34 ºC) to evaluate volume. Broiler was influenced by type. Birds visited bell less often, but presented higher total per visit...

This study assessed the effect of both embryonic thermal manipulation and dietary threonine level on response broilers inoculated with Salmonella Enteritidis, considering bacterial counts in cecal contents, intestinal morphology, mucin heat shock protein 70 gene expression, body weight gain. Thermal was used from 11 days incubation until hatch, defining three treatments: standard (37.7°C), continuous high temperature (38.7°C) low (36.7°C). After chicks were distributed according to a 3x2+1...

The increasing number of studies reporting the presence Salmonella in environmental water sources suggests that it is beyond incidental findings originated from sparse fecal contamination events. However, there no consensus on occurrence as its relative serovar representation across non-recycled sources. We conducted a meta-analysis proportions by fitting random-effects model using restricted maximum-likelihood estimator to obtain weighted average proportion and between-study variance...

Abstract This study evaluated the expression of heat shock protein 70 kD (hsp70) in broiler chicken embryos subjected to cold (Experiment I) or high incubation temperature II). In each experiment, fertile eggs were distributed three incubators kept at 37.8°C. At day 13 (D13), D16, and D19 incubation, acute (32°C) (40°C) for 4–6 hr. Immediately after exposure, samples from liver, heart, breast muscle, brain, lungs 40 taken per age treatment (control stressed embryos). A tissue pool 10 was...

This study aimed to estimate the energy requirements for maintenance and gain of Japanese European quails under growth housed in two rearing systems: floor pens cages. To determine requirements, experiments were conducted with birds cages environmental chambers (experiment 1) at room temperature conditions 2). The experimental design was completely randomized four levels feed supply (100, 75, 50 25%) repetitions. Energy estimated by comparative slaughter method through a feeding trial. In...

The objective of this study was to evaluate the effects 1% dietary glutamic acid on body weight, intestinal morphometry, and anti-Newcastle antibody titers broiler chickens submitted heat stress. One-d-old male chicks (n=120) were distributed according a 2 x factorial design with two environmental temperatures (thermoneutral or stress) diets (with 0 acid). Heat stress temperature constantly maintained (24h/day) 5 ºC higher than thermoneutral temperature. Diets supplied nutritional...

The effects of in ovo feeding with threonine (Thr) on intestinal morphology, ileal gene expression and performance broiler chicken between 1 21 d age (d) were assessed. On day 17.5 incubation, fertile eggs randomly allotted to 5 treatments Thr injection the amniotic fluid (0; 1.75; 3.5; 5.25; 7%, corresponding 17.5; 35; 52.5 70 mg Thr/mL). After hatch, chicks given a commercial corn-soybean diet up d. Daily feed intake (FI), body weight (BW), food conversion ratio (FCR) measured from 7, 14,...

This study was carried out with the objective of evaluating effect heat (38.8°C) or cold (35.8°C) stress on chicken embryo development and tissues Hsp70 levels, after 13th day incubation. Embryo weight (percent egg weight), organ weight) levels (ng mg –1 total protein) in different (liver, breast muscle, heart, lungs, brain kidney) were studied at end Cold induced a lower kidney lungs weights, whereas heart liver lighter heat-stressed embryos. An interaction between temperature age obtained...

The aim of this study was to gain information on quality traits, mainly bacterial and somatic cell counts bulk milk, produced by small- medium-scale producers in a semiarid northeastern region Brazil identify characterize possible risk factors associated with those traits. A cross-sectional performed 50 farms. Bulk milk samples were collected for counts. Additionally, about farm demographics, general management practices, hygiene, milking procedures also obtained. Multivariable analysis...

To evaluate mannan oligosaccharide (MOS) and threonine effects on performance, small intestine morphology Salmonella spp. counts in Enteritidis-challenged birds.One-day-old chicks (1d) were distributed into five treatments: nonchallenged animals fed basal diet (RB-0), infected with Enteritidis (RB-I), high level of (HT-I), birds MOS (MOS-I), (HT+MOS-I). Birds inoculated at 2d Enteritidis, except RB-0 birds. Chicks higher dietary showed performance similar to intestinal recovery 8 dpi. the...

With the objective of evaluating effect reducing crude protein (CP) and L-glutamic acid supplementation, Dekalb Brown laying hens with 34 weeks age. The birds were distributed in six treatments a completely randomized design, eight replications birds. control diet contained 17% CP. second third had CP reduced by 1.5 3.0 percentage points (pp) relation to diet, respectively. In fourth treatment, was added same amount glutamate (2.982%), last two 1 2 pp (3.982 4.98%), treatment 15.17% + 0.341%...

This trial was designed to evaluate the off-label use of ceftiofur with Marek's vaccine in one-day-old broiler chicks, a prophylactic treatment that has been done some commercial hatcheries, on emergence extended-spectrum beta-lactamase producing Escherichia coli (ESBL-E. coli). A total 168 chicks (Cobb500®) were used completely randomized design. Birds assigned two treatments (Marek's plus saline vs ceftiofur) and six repetitions, 14 animals each. Cloacal swabs collected from 1 days...

To investigate the genetic context of colistin resistance in anmcr-9-harbouring Salmonella Typhimurium ST19 strain from swine Brazil. Minimum inhibitory concentrations (MIC) to were determined by broth microdilution. Whole-genome sequencing was performed on an Illumina MiSeq system, followed de novo genome assembly using SPAdes 1.13.1. The draft sequence annotated Prokka KBase online server. Downstream analyses for resistome and plasmid detection tools available at Center Genomic...

ABSTRACT A Saccharomyces cerevisiae -expressed nucleocapsid (N) polypeptide of the M41 strain infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, it compared favorably with commercial ELISA.

This experiment was conducted to evaluate the effect of levels calcium (Ca) and particle size limestone (LS) for laying hens. It used 216 Dekalb White hens at 25 49 weeks age in a completely randomized design 3 × 2 factorial arrangement, composed three (3.92, 4.02 4.12%) two sizes (thin - 0.60 mm; thick 1.00 mm), resulting 6 treatments with six replicates birds. There no significant neither interaction feed intake, egg weight, specific gravity, shell thickness, percentage, Haugh unit,...

Although Staphylococcus aureus has been implicated as the main species causing human food poisoning, recent studies have shown that coagulase-negative could also harbor enterotoxin-encoding genes. Such organisms are often present in goat milk and most important mastitis-causing agents. Therefore, this study aimed to investigate occurrence of genes among coagulase-positive (CoPS) (CoNS) staphylococci isolated from raw produced semi-arid region Paraiba, for production Brazil....

Biofilm-associated antimicrobial resistance is of increasing importance to the maintenance and spread foodborne pathogens in food industry. This study aimed investigate ability form biofilm staphylococci contaminating small-scale goat milk dairy plants.Sixty isolates were tested for against 20 drugs by microdilution method. Biofilm-forming traits assessed microtiter plate method (MtP), Congo red agar (CRA), icaD gene detection polymerase chain reaction (PCR).High ampicillin (60/60; 100%),...

The prophylactic administration of ceftiofur to newly hatched chicks is a common practice in some hatcheries worldwide mitigate early gastrointestinal infections caused by Enterobacteriaceae. In spite the crucial role gut microbiome for broiler's health, there still limited information on how microbial composition affected such procedure. We investigated effects posthatch application cecal microbiota 14-day-old broilers fed regular or sanguinarine-supplemented diets. DNA samples were...