A5006805023- Extracellular vesicles in disease
- Developmental Biology and Gene Regulation
- Axon Guidance and Neuronal Signaling
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Photoacoustic and Ultrasonic Imaging
- Neurobiology and Insect Physiology Research
- Advanced Electron Microscopy Techniques and Applications
- Congenital heart defects research
- Protist diversity and phylogeny
- Evolutionary Game Theory and Cooperation
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Nanopore and Nanochannel Transport Studies
- Multilevel Inverters and Converters
- Parathyroid Disorders and Treatments
- Freezing and Crystallization Processes
- Physiological and biochemical adaptations
- thermodynamics and calorimetric analyses
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Parkinson's Disease Mechanisms and Treatments
- Neurological Disease Mechanisms and Treatments
- Silk-based biomaterials and applications
Rockefeller University
2015-2024
Weill Cornell Medicine
2017
Cornell University
2017
Rutgers, The State University of New Jersey
2007-2009
Johnson University
2006-2009
Significance Increasing evidence suggests that extracellular vesicles (EVs) can transfer genetic material to recipient cells. However, the mechanism and role of this phenomenon are largely unknown. Here we have made a remarkable discovery: EVs harbor full mitochondrial genome. These in turn their mtDNA cells with impaired metabolism, leading restoration metabolic activity. We determined hormonal therapy induces oxidative phosphorylation-deficient breast cancer cells, which be rescued via...
Numerous epidemiological studies link vascular disorders, such as hypertension, diabetes mellitus, and stroke, with Alzheimer's disease (AD). Hypertension, specifically, is an important modifiable risk factor for late-onset AD. To examine the between midlife hypertension onset of AD later in life, we chemically induced chronic TgSwDI mouse model early adulthood. Hypertension accelerated cognitive deficits Barnes maze test (P<0.05 after 3 months treatment; P<0.001 6 months), microvascular...
During Drosophila melanogaster heart development, a lumen forms between apical surfaces of contralateral cardioblasts (CBs). We show that Slit and its receptor Roundabout (Robo) are required at CB domains for formation. Mislocalization outside the domain causes ectopic formation mislocalization cell junction proteins, E-cadherin (E-Cad) Enabled, without disrupting overall polarity. Ectopic is suppressed in robo mutants, which indicates robo's requirement this process. Genetic evidence...
Heart morphogenesis requires the coordinated regulation of cell movements and cell-cell interactions between distinct populations cardiac precursor cells. Little is known about mechanisms that organize cells into this complex structure. In study, we analyzed role Slit, an extracellular matrix protein its transmembrane receptors Roundabout (Robo) Roundabout2 (Robo2) during Drosophila heart tube, a process analogous to early formation in vertebrates. During assembly, two types progenitor align...
Microbes living in stagnant water typically rely on chemical diffusion to draw nutrients from their environment. The sulfur-oxidizing bacterium Thiovulum majus and the ciliate Uronemella have independently evolved ability form a ‘veil’, centimetre-scale mucous sheet which cells organize produce macroscopic flow. This flow pulls through community an order of magnitude faster than diffusion. To understand how natural selection led these microbes evolve this collective behaviour, we connect...
In this study we examined Roundabout signaling in the Drosophila embryonic hindgut.Slit and its receptors (Robo) 2 (Robo2) localize to discrete regions hindgut epithelium surrounding visceral mesoderm. Loss of robo, robo2 or slit did not disrupt overall patterning. However, robo mutants showed a decrease microvillus length on boundary cells epithelium. Rescue overexpression analysis revealed that is specifically required mesoderm for correct underlying Expression mutant embryos restored...
The morphogenesis of the Drosophila embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and shape changes during development. One challenges faced in this structure is that lumen tube, well membrane features are crucial to formation, difficult visualize whole mount embryos, due small size intra-lumenal space relative embryo. use transmission electron microscopy allows higher magnification these structures gives advantage examining...
This article presents methods for generating in vitro fibrin clots and analyzing the effect of betaamyloid (Aβ) protein on clot formation structure by spectrometry scanning electron microscopy (SEM).Aβ, which forms neurotoxic amyloid aggregates Alzheimer's disease (AD), has been shown to interact with fibrinogen.This Aβ-fibrinogen interaction makes structurally abnormal resistant fibrinolysis.Aβ-induced abnormalities clotting may also contribute cerebrovascular aspects AD pathology such as...
A picture is worth a thousand words. Correlative light and electron microscopy (CLEM) valuable imaging tool as it bridges the resolution gap between two modalities. Fluorescent markers are applied routinely to capture broad field of view in two-dimensional space (2D), 3D, or 4D. In diffraction-limited (LM), approaches 200 nm. Alternatively, physical properties an beam allow improvement approaching 1 nm for visualization ultrastructural features, including surrounding architecture some...
Abstract Scanning electron microscopy (SEM) offers an unparalleled view of the membrane topography mammalian cells by using a conventional osmium (OsO 4 ) and ethanol-based tissue preparation. However, SEM methods limit optimal resolution due to ethanol lipid interactions interfere with visualization fluorescent reporter proteins. Therefore, correlative light (CLEM) has been hindered adverse effects OsO on retention fluorescence signals. To overcome this technological gap in achieving...
The morphogenesis of the Drosophila embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and shape changes during development. One challenges faced in this structure is that lumen tube, well membrane features are crucial to formation, difficult visualize whole mount embryos, due small size intra-lumenal space relative embryo. use transmission electron microscopy allows higher magnification these structures gives advantage examining...
Journal Article A Freeze Drying Sample Preparation Method for Correlative Light and Scanning/Transmission Electron Microscopy Get access Kunihiro Uryu, Uryu Resource Center, The Rockefeller University, New York, NY, USA Search other works by this author on: Oxford Academic Google Scholar Nadine Soplop, Soplop Devrim Acehan, Acehan Charles M Rice, Rice Center the Study of Hepatitis C, Laboratory Virology Infectious Disease, Maria Teresa Catanese, Catanese USADepartment Diseases, King's...