A5045312922- Virus-based gene therapy research
- Microtubule and mitosis dynamics
- interferon and immune responses
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- Cell Image Analysis Techniques
- Viral Infections and Vectors
- Autophagy in Disease and Therapy
- Adenosine and Purinergic Signaling
- 14-3-3 protein interactions
- Phagocytosis and Immune Regulation
- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- AI in cancer detection
- Biotin and Related Studies
- Medical Imaging Techniques and Applications
- Cellular transport and secretion
- Single-cell and spatial transcriptomics
- Click Chemistry and Applications
- Advanced X-ray Imaging Techniques
Memorial Sloan Kettering Cancer Center
2016-2023
Mitosis occurs efficiently, but when it is disturbed or delayed, p53-dependent cell death senescence often triggered after mitotic exit. To characterize this process, we conducted CRISPR-mediated loss-of-function screens using a cell-based assay in which mitosis consistently by centrosome loss. We identified 53BP1 and USP28 as essential components acting upstream of p53, evoking p21-dependent cycle arrest response not only to loss, also other distinct defects causing prolonged mitosis....
Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of centriole that mediate membrane docking during ciliogenesis, marking cilia base around ciliary gate. Here we determine a super-resolved multiplex 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit cone-shaped architecture with components filling space between each pinwheel blade, new structural element term appendage matrix (DAM). Specifically, CEP83,...
Subdistal appendages (sDAPs) are centriolar elements that observed proximal to the distal (DAPs) in vertebrates. Despite obvious presence of sDAPs, structural and functional understanding them remains elusive. Here, by combining super-resolved localization analysis CRISPR-Cas9 genetic perturbation, we find although DAPs sDAPs primarily responsible for distinct functions ciliogenesis microtubule anchoring, respectively, one element actually affects positioning other. Specifically, dual layers...
Publications involving fluorescent microscopy images generally contain many panels with split channels, merged images, scale bars and label text. Similar layouts of are used when displaying other electron micrographs, photographs, images. Assembling editing these figures even spacing, consistent font, text position, accurate bars, features can be tedious time consuming. In order to save time, I have created a toolset ImageJ Plugin called QuickFigures. QuickFigures includes helpful that...
Phosphatidylserine (PS) is exposed on the surface of apoptotic cells and known to promote immunosuppressive signals in tumor microenvironment (TME). Antibodies that block PS interaction with its receptors have been shown repolarize TME into a proinflammatory state. Radiation therapy (RT) an effective focal treatment isolated solid tumors but less at controlling metastatic cancers. We found tumor-directed RT caused increase expression viable immune infiltrates mouse B16 melanoma. hypothesize...
Effective depletion of immune suppressive regulatory T cells (Tregs) in the tumor microenvironment without triggering systemic autoimmunity is an important strategy for cancer immunotherapy. Modified vaccinia virus Ankara (MVA) a highly attenuated, non-replicative with long history human use. Here, we report rational engineering immune-activating recombinant MVA (rMVA, MVA∆E5R-Flt3L-OX40L) deletion E5R gene (encoding inhibitor DNA sensor cyclic GMP-AMP synthase, cGAS) and expression two...
Abstract Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of centriole that mediate membrane docking during ciliogenesis, marking cilia base around ciliary gate. Here, we determined a superresolved multiplex 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit cone-shaped architecture with components filling space between each pinwheel blade, new structural element termed appendage matrix (DAM)....
Abstract Publications involving fluorescent microscopy generally contain many panels with split channels, merged images, scale bars and label text. Assembling editing these figures even spacing, consistent font, text position, accurate other features can be tedious time consuming. In order to save streamline the process I have created a toolset called QuickFigures.
Abstract Novel strategies to reprogram tumor-infiltrating myeloid cells for cancer immunotherapy are urgently needed, given that the primary and acquired resistance immune checkpoint blockade (ICB) therapy has hindered overall success of immunotherapy. Modified vaccinia virus Ankara (MVA) is a highly attenuated, non-replicative an approved vaccine against smallpox monkeypox. Here we report rational engineering recombinant MVA, MQ833, by removing three suppressive genes, E5R, E3L, WR199, from...
Summary Intratumoral (IT) delivery of immune-activating viruses can serve as an important strategy to turn “cold” tumors into “hot” tumors, resulting in overcoming resistance immune checkpoint blockade (ICB). Modified vaccinia virus Ankara (MVA) is a highly attenuated, non-replicative that has long history human use. Here we report IT recombinant MVA (rMVA), lacking E5R encoding inhibitor the DNA sensor cyclic GMP-AMP synthase (cGAS), expressing dendritic cell growth factor, Fms-like...
<h3>Background</h3> Oncolytic viruses are promising therapeutic agents for advanced cancers because of their ability to induce antitumor innate and adaptive immunity. Modified vaccinia virus Ankara (MVA) is an attenuated, replication-deficient poxvirus safe human use, making it a favorable platform cancer immunotherapy. Previously we discovered the E5R gene in MVA encodes inhibitor cGAS/STING-mediated cytosolic DNA-sensing pathway. The engineered deleting expressing dendritic cell (DC)...