A5068196506- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Biofuel production and bioconversion
- Effects of Radiation Exposure
- Hematopoietic Stem Cell Transplantation
- PARP inhibition in cancer therapy
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Robotic Mechanisms and Dynamics
- Advanced Electron Microscopy Techniques and Applications
- Metalloenzymes and iron-sulfur proteins
- Cellular Mechanics and Interactions
- Microbial Metabolic Engineering and Bioproduction
- Cellular transport and secretion
Memorial Sloan Kettering Cancer Center
2012-2021
Cancer Research UK
2009-2014
The Honourable Society of Lincoln's Inn
2009-2014
UNSW Sydney
2004
The complete set of viable deletion strains in Saccharomyces cerevisiae was screened for sensitivity mutants to five oxidants identify cell functions involved resistance oxidative stress. This screen identified a unique mainly constitutive providing the first line defense against particular oxidant; these are very dependent on nature oxidant. Most distinct from those repair and recovery damage, which generally induced response stress, because there little correlation between mutant reported...
Mitosis occurs efficiently, but when it is disturbed or delayed, p53-dependent cell death senescence often triggered after mitotic exit. To characterize this process, we conducted CRISPR-mediated loss-of-function screens using a cell-based assay in which mitosis consistently by centrosome loss. We identified 53BP1 and USP28 as essential components acting upstream of p53, evoking p21-dependent cycle arrest response not only to loss, also other distinct defects causing prolonged mitosis....
Wee1 is a protein kinase that negatively regulates mitotic entry in G2 phase by suppressing cyclin B-Cdc2 activity, but its spatiotemporal regulations remain to be elucidated. We observe the dynamic behavior of Schizosaccharomyces pombe cells and manipulate localization activity study function. At late G2, nuclear efficiently suppresses around spindle pole body (SPB). During G2/M transition when highly enriched at SPB, temporally accumulates face SPB B-Cdc2-dependent manner locally both...
After 9/11, threat of nuclear attack on American urban centers prompted government agencies to develop medical radiation countermeasures mitigate hematopoietic acute syndrome (H-ARS) and higher-dose gastrointestinal (GI-ARS) lethality. While repurposing leukemia drugs that enhance bone marrow repopulation successfully treats H-ARS in preclinical models, no mitigator potentially deliverable under mass casualty conditions preserves GI tract. Here, we report generation an anti-ceramide 6B5...
The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role bipolar assembly. However, it remains elusive how proteins are recruited centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within domain required for localisation. Critically, these sequences essential functions of Alp7, including proper formation progression. Moreover, identified...
Cells treated with low doses of linoleic acid hydroperoxide (LoaOOH) exhibit a cell-cycle delay that may provide mechanism to overcome oxidative stress. Strains sensitive LoaOOH from the genome-wide deletion collection were screened identify deletants in which phenotype was reduced. Forty-seven identified unable mount normal response, implicating product deleted gene oxidant-mediated wild-type. Of these genes, SWI6 particular interest due its role progression through Start. The swi6 deletant...
Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted pcp1, and can be suited any genes involved other processes.
Centriole-to-centrosome conversion (CCC) safeguards centriole homeostasis by coupling duplication with segregation, and is essential for stabilization of mature vertebrate centrioles naturally devoid the geometric scaffold or cartwheel. Here we identified PPP1R35, a putative regulator protein phosphatase PP1, as novel centriolar required CCC. We found that PPP1R35 enriched at newborn daughter in S G2 phase. In absence assembly initiates normally phase, but none nascent can form active...
The spindle pole body (SPB) in fungi is the equivalent of animal centrosome. A number previous studies have identified many, if not all, components SPB. SPB structural platform for microtubule nucleation and plays important roles, both mitosis meiosis. absolutely essential cell survival its abnormalities give rise to aberrant division morphogenesis. Therefore, it crucial understand how organises itself functions individual are regulated. We report here a procedure generate temperature...
The spindle pole body (SPB) in fungi is the equivalent of animal centrosome. A number previous studies have identified many, if not all, components SPB. SPB structural platform for microtubule nucleation and plays important roles, both mitosis meiosis. absolutely essential cell survival its abnormalities give rise to aberrant division morphogenesis. Therefore, it crucial understand how organises itself functions individual are regulated. We report here a procedure generate temperature...
Abstract Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes spindle pole body (SPB) component Schizosaccharomyces pombe . This manipulation is not restricted analysis Pcp1, and can be suited any genes involved other processes.