A5101473859- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Molecular Biology Techniques and Applications
- Respiratory viral infections research
- Cerebrovascular and Carotid Artery Diseases
- vaccines and immunoinformatics approaches
- Hepatitis B Virus Studies
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Retinal and Optic Conditions
- Elasticity and Material Modeling
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- Biochemical and Molecular Research
- Diabetes and associated disorders
- Cervical Cancer and HPV Research
- Viral Infections and Vectors
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- SARS-CoV-2 detection and testing
- Cell Image Analysis Techniques
German Cancer Research Center
2001-2024
Heidelberg University
2024
Friedrich-Loeffler-Institut
1990-2024
University Hospital Ulm
2021
University of Göttingen
2020
Loyola University Chicago
1996-1997
SUMMARY The global spread of SARS-CoV-2/COVID-19 is devastating health systems and economies worldwide. Recombinant or vaccine-induced neutralizing antibodies are used to combat the COVID-19 pandemic. However, recently emerged SARS-CoV-2 variants B.1.1.7 (UK), B.1.351 (South Africa) B.1.1.248 (Brazil) harbor mutations in viral spike (S) protein that may alter virus-host cell interactions confer resistance inhibitors antibodies. Here, using pseudoparticles, we show entry UK, South Africa...
Severe acute respiratory syndrome coronavirus 2 cell entry depends on angiotensin-converting enzyme and transmembrane serine protease is blocked in culture by camostat mesylate, a clinically proven inhibitor. Whether mesylate able to lower disease burden 2019 sepsis currently unknown.Retrospective observational case series.Patient treated ICU of University hospital Göttingen, Germany.Eleven critical ill patients with organ failure were ICU.Compassionate use (six patients, group) or...
The interplay between feeding and excretion is essential for organismal nutrition survival, yet their mechanistic coupling remains poorly understood. At the onset of life, must be initiated while developmental waste products — meconium need to eliminated. Using Drosophila as a model system, we explored in vivo mechanisms coordinating these processes. We developed novel behavioral assays newly eclosed flies discovered that, similar neonatal mammals, excrete shortly after eclosion. Remarkably,...
RNA viruses present a constant threat to human health, often with limited options for vaccination or therapy. Notable examples include influenza and coronaviruses, which have pandemic potential. Filo- henipaviruses cause more outbreaks, but high case fatality rates. All rely on the activity of virus-encoded RNA-dependent polymerase (RdRp). An antiviral nucleoside analogue, 4'-Fluorouridine (4'-FlU), targets RdRp diminishes replication several viruses, including A virus SARS-CoV-2, through...
The visualization of viral pathogens in infected tissues is an invaluable tool to understand spatial virus distribution, localization, and cell tropism vivo. Commonly, virus-infected are analyzed using conventional immunohistochemistry paraffin-embedded thin sections. Here, we demonstrate the utility volumetric three-dimensional (3D) immunofluorescence imaging tissue optical clearing light sheet microscopy investigate host–pathogen interactions pandemic SARS-CoV-2 ferrets at a mesoscopic...
Although most non-segmented negative-strand RNA viruses (NNSVs) replicate in the cytoplasm, NNSV proteins often exert host manipulatory functions nucleus. Matrix (M) of henipaviruses and other paramyxoviruses shuttle through nucleus, where factors may bind for M modification or host-cell manipulation. Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) is an interactor Hendra Nipah virus M. Both accumulate nucleus ANP32B-dependent manner. Here we demonstrate that...
Eradication of cancer cells by the immune system requires extravasation, infiltration and progression through tumor extracellular matrix (ECM). These are also critical determinants for successful adoptive cell immunotherapy solid tumors. Together with structural proteins, such as collagens fibronectin, heparan sulfate (HS) proteoglycans major components ECM. Heparanase 1 (HPSE) is only enzyme known to have endoglycosidase activity that degrades HS. HPSE expressed at high levels in almost all...
Respiratory tract epithelium infection plays a primary role in Nipah virus (NiV) pathogenesis and transmission. Knowledge about dynamics host responses to NiV respiratory epithelia is scarce. Studies non-differentiated cells or cell lines indicate insufficient interferon (IFN) responses. However, studies are lacking the determination of complex response patterns differentiated for understanding replication spread swine. Here we characterized porcine bronchial epithelial (PBEC) cultivated at...
Abstract The visualization of viral pathogens in infected tissues is an invaluable tool to understand spatial virus distribution, localization, and cell tropism vivo . Commonly, virus-infected are analyzed using conventional immunohistochemistry paraffin-embedded thin sections. Here, we demonstrate the utility volumetric three-dimensional (3D) immunofluorescence imaging tissue optical clearing light sheet microscopy investigate host-pathogen interactions pandemic SARS-CoV-2 ferrets at a...
Zusammenfassung SARS-CoV-2 ist eine neuartige humanpathogene Coronavirus-Variante, deren Prädilektion für den Atemwegstrakt zu einer raschen pandemischen Verbreitung durch viruspartikelhaltige Aerosole geführt hat. Die individuelle Organsuszeptibilität wird maßgeblich die Besatzdichte mit dem membranständigen Rezeptormolekül ACE2 bestimmt, das als zentraler Interaktionspartner virale Spike-Protein Adhäsions- und Fusionsprozess vermittelt somit Grundvoraussetzung zur Aufnahme des Virusgenoms...