A5016148855- Osteoarthritis Treatment and Mechanisms
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Temporomandibular Joint Disorders
- Cancer-related molecular mechanisms research
- Bone Tumor Diagnosis and Treatments
- Signaling Pathways in Disease
- Bone Metabolism and Diseases
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Musculoskeletal synovial abnormalities and treatments
- Proteoglycans and glycosaminoglycans research
- NF-κB Signaling Pathways
- Winter Sports Injuries and Performance
- Bone and Joint Diseases
- Tendon Structure and Treatment
- Heterotopic Ossification and Related Conditions
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Knee injuries and reconstruction techniques
- Adipose Tissue and Metabolism
- Connective tissue disorders research
- Renal Diseases and Glomerulopathies
- Telomeres, Telomerase, and Senescence
Xuan Wu Hospital of the Capital Medical University
2024
National Clinical Research
2023-2024
Capital Medical University
1997-2023
National Clinical Research Center for Digestive Diseases
2022-2023
University of Massachusetts Chan Medical School
2018-2020
Peking University
2009-2017
Brigham and Women's Hospital
2016
Harvard University
2016
University of Trieste
1997
Institute for In Vitro Sciences
1997
Objective Proinflammatory molecules promote osteoclast‐mediated bone erosion by up‐regulating local RANKL production. However, recent evidence suggests that combinations of cytokines, such as tumor necrosis factor (TNF) plus interleukin‐6 (IL‐6), induce RANKL‐independent osteoclastogenesis. The purpose this study was to better understand TNF/IL‐6–induced osteoclast formation and determine whether RANK is absolutely required for osteoclastogenesis in murine inflammatory arthritis. Methods...
Abstract The osteoblast differentiation capacity of skeletal stem cells (SSCs) must be tightly regulated, as inadequate bone formation results in low mass and fragility, over-exuberant osteogenesis heterotopic ossification (HO) soft tissues. RUNX2 is essential for tuning this balance, but the mechanisms posttranslational control remain to fully elucidated. Here, we identify that a CK2/HAUSP pathway key regulator stability, Casein kinase 2 (CK2) phosphorylates RUNX2, recruiting deubiquitinase...
Abstract Objective To determine the possible involvement and regulatory mechanisms of Wnt‐5A signaling in interleukin‐1β (IL‐1β)–induced increase matrix metalloproteinase 1 (MMP‐1), MMP‐3, MMP‐9, MMP‐13 expression temporomandibular joint (TMJ) condylar chondrocytes. Methods Primary rabbit chondrocytes were treated with IL‐1β, purified protein, or both transfected vector. Expression Wnt‐5A, MMP‐1, MMP‐13, type II collagen, as well cell morphologic changes, examined. explore action...
The origin and differentiation mechanism of articular chondrocytes remain poorly understood. Broadly, the difference in developmental mechanisms growth-plate cartilage is still less elucidated. Here, we identified that nuclear factor activated T-cells cytoplasmic 1 (NFATc1) a crucial regulator articular, but not growth-plate, chondrocyte during development. At early stage mouse knee development (embryonic day 13.5), NFATc1-expressing cells were mainly located flanking region joint interzone....
Abstract Background To investigate the prognostic value of ferroptosis-related long noncoding RNAs (lncRNAs) in oral squamous cell carcinoma (OSCC) and to construct a risk immune activity model. Methods We obtained clinical RNA-seq information on OSCC patient data The Cancer Genome Atlas (TCGA) Data Sharing (GDC) portal. Through combination differential analysis, Pearson correlation analysis Cox regression lncRNAs were identified, model was established based these lncRNAs. accuracy evaluated...
Cartilage acidic protein 1 (CRTAC1) was recently identified as an elevated in the synovial fluid of patients with osteoarthritis (OA) by a proteomic analysis. This gene is also upregulated both human and mouse OA transcriptomic The objective this study to characterize expression function CRTAC1 OA. Here, we first confirm increase cartilage biopsies from undergoing joint replacement real-time PCR immunohistochemistry. Furthermore, report that proinflammatory cytokines interleukin-1beta tumor...
Osteochondromas are common benign osteocartilaginous tumors in children and adolescents characterized by cartilage-capped bony projections on the surface of bones. These often cause pain, deformity, fracture, musculoskeletal dysfunction, they occasionally undergo malignant transformation. The pathogenesis osteochondromas remains poorly understood. Here, we demonstrate that nuclear factor activated T cells c1 c2 (NFATc1 NFATc2) suppress osteochondromagenesis through individual combinatorial...
BACKGROUND: Tripterygium wilfordii Hook F is a medicinal plant used for the treatment of glomerulonephritis in China. We studied effect multiglycoside (TWG) on glomerular albumin permeability (Palbumin) vitro. METHODS: Isolated rat glomeruli were incubated with protamine (600 micrograms/ml) 30 min, or human recombinant tumour necrosis factor (TNF-alpha 0.4 ng/ml), superoxide (10 units/ml), serum from focal segmental sclerosis (FSGS) patient 10 min at 37 degrees C. TWG, 1 mg/ml, was added...
The dysfunction of the cellular endolysosomal pathway, such as in lysosomal storage diseases, can cause severe musculoskeletal disorders. However, how causes abnormalities remains poorly understood, limiting therapeutic options. Here, we report that CHMP5, a member endosomal sorting complex required for transport (ESCRT)-III protein family, is essential to maintain pathway and regulate bone formation osteogenic lineage cells. Genetic ablation Chmp5 mouse cells increases vivo vitro....
The dysfunction of the cellular endolysosomal pathway, such as in lysosomal storage diseases, can cause severe musculoskeletal disorders. However, how causes abnormalities remains poorly understood, limiting therapeutic options. Here, we report that CHMP5, a member endosomal sorting complex required for transport (ESCRT)-III protein family, is essential to maintain pathway and regulate bone formation osteogenic lineage cells. Genetic ablation Chmp5 mouse cells increases vivo vitro....
In the musculoskeletal system, stem cells generating enveloping tissues, such as synovial joints, periosteum, and muscle fascia, remain poorly understood. Broadly, lineage correlation among these those osteogenic growth plate inside bone is also elusive. Here we identify a migratory, periarticular cell population enriched by transcriptional factor NFATc1 (named N1-SSCs), which contributes to formation of all tissues moves into partial strikingly bone-resorbing osteoclasts too. Beyond...
Our previous study in genetic mouse models found that NFATc1 and NFATc2 suppress osteochondroma formation from entheseal progenitors. However, it remains unclear whether NFAT signaling is also involved human osteochondromagenesis. As the first step addressing this question, current aimed to determine expression patterns of NFATC1 NFATC2 samples.Immunohistochemistry (IHC) was used examine analyze samples. The periosteum map under physiological conditions by IHC. Furthermore, periosteal...
Abstract The dysfunction of the cellular endolysosomal pathway, such as in lysosomal storage diseases, can cause severe musculoskeletal disorders. However, how causes abnormalities remains poorly understood, limiting therapeutic options. Here, we report that CHMP5, a member endosomal sorting complex required for transport (ESCRT)-III protein family, is essential to maintain pathway and regulate bone formation osteogenic lineage cells. Genetic ablation Chmp5 mouse cells increases vivo vitro....
Abstract The origin and differentiation mechanism of articular chondrocytes remain poorly understood. Broadly, the difference in developmental mechanisms growth-plate cartilage is still less elucidated. Here, we identified that nuclear factor activated T-cells cytoplasmic 1 (NFATc1) a crucial regulator articular, but not growth-plate, chondrocyte during development. At early stage mouse knee development (embryonic day 13.5), NFATc1-expressing cells were mainly located flanking region joint...