A5071540168- Inflammatory Bowel Disease
- Nanoplatforms for cancer theranostics
- Microscopic Colitis
- Photodynamic Therapy Research Studies
- Gut microbiota and health
- Esophageal Cancer Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Colorectal Cancer Screening and Detection
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Biosensors and Analytical Detection
- Radiomics and Machine Learning in Medical Imaging
- Peptidase Inhibition and Analysis
- Photoacoustic and Ultrasonic Imaging
- Immune cells in cancer
- Gastric Cancer Management and Outcomes
- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Phytochemistry and biological activity of medicinal plants
- Veterinary medicine and infectious diseases
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Pancreatic and Hepatic Oncology Research
Stanford University
2016-2025
Institute of Biocybernetics and Biomedical Engineering
2024
Polish Academy of Sciences
2024
University of California, San Diego
2024
Palo Alto University
2020
Stanford Medicine
2015-2017
Clark University
2016
National Cancer Center
2015
Catholic University of Korea
2015
Ajou University
2015
The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability not been described. Here we report design and vivo demonstration miniature, non-contact, opto-electro-mechanical as an accessory clinical endoscopes that can provide multiplexed molecular data via panel SERS NPs. This enables...
Atherosclerosis is a major cause of global morbidity and mortality that could benefit from novel targeted therapeutics. Recent studies have shown efficient local drug delivery with nanoparticles, although the nanoparticle targeting mechanism for atherosclerosis has not yet been fully elucidated. Here we used in vivo ex multimodal imaging to examine permeability vessel wall atherosclerotic plaque accumulation fluorescently labeled liposomal nanoparticles rabbit model. We found strong...
Neutrophils have crucial antimicrobial functions but are also thought to contribute tissue injury upon exposure bacterial products, such as lipopolysaccharide (LPS). To study the role of neutrophils in LPS-induced endotoxemia, we developed a new mouse model, PMNDTR mice, which injection diphtheria toxin induces selective neutrophil ablation. Using this found, surprisingly, that serve protect host from lethal inflammation. This protective was observed conventional and germ-free animal...
Although literature suggests that resistance to TNF inhibitor (TNFi) therapy in patients with ulcerative colitis (UC) is partially linked immune cell populations the inflamed region, there still substantial uncertainty underlying relevant spatial context. Here, we used highly multiplexed immunofluorescence imaging technology CODEX create a publicly browsable tissue atlas of inflammation 42 regions from 29 UC and 5 healthy individuals. We analyzed 52 biomarkers on 1,710,973 spatially resolved...
Cancers of the gastrointestinal (GI) tract are among most frequent and lethal cancers worldwide. An important reason for this high mortality is that early disease typically asymptomatic, patients often present with advanced, incurable disease. Even in high-risk who routinely undergo endoscopic screening, lesions can be missed due to their small size or subtle appearance. Thus, current imaging approaches lack sensitivity specificity accurately detect incipient GI cancers. Here we report our...
Significance Early detection by white-light endoscopy (WLE) is the key procedure used in clinical care to prevent colorectal cancer (CRC). In high-risk patients develop CRC, like inflammatory bowel disease, miss-rate of dysplastic lesions three times higher compared healthy individuals. this study we explored use a near-infrared probe with fluorescence as an adjunct WLE highlight adenomas. We demonstrated clinically relevant animal models carcinogenesis and ulcerative colitis that our...
Accidental injury to the cardiac conduction system (CCS), a network of specialized cells embedded within heart and indistinguishable from surrounding muscle tissue, is major complication in surgeries. Here, we addressed this unmet need by engineering targeted antibody-dye conjugates directed against CCS, allowing for visualization CCS vivo following single intravenous injection mice. These optical imaging tools showed high sensitivity, specificity, resolution, with no adverse effects on...
Abstract Background Resistance to tumor necrosis factor inhibitor (TNFi) therapy remains a major obstacle in managing ulcerative colitis (UC), affecting up 50% of patients.1 The complex interplay immune cells and their spatial organization the gut mucosa is poorly understood but likely contributes therapeutic outcomes.1,2 This study utilized advanced multiplexed imaging construct atlas UC, providing insights into cellular interactions driving TNFi resistance. Novel omic technologies show be...
Abstract Early and comprehensive endoscopic detection of colonic dysplasia—the most clinically significant precursor lesion to colorectal adenocarcinoma—provides an opportunity for timely, minimally invasive intervention prevent malignant transformation. Here, the development evaluation biodegradable near‐infrared fluorescent silica nanoparticles (FSN) that have potential improve adenoma during fluorescence‐assisted white‐light colonoscopic surveillance in rodent human‐scale models...
Pancreatic ductal adenocarcinoma (PDAC) is the most common pancreatic tumor and associated with poor prognosis treatment response. The microenvironment (TME) recognized as an important factor in metastatic progression across cancers. Despite extensive study of TME PDAC, cellular molecular signaling networks remain poorly understood, largely due to tremendous heterogeneity tumors. While earlier work characterized PDAC immunologically privileged by immune system, recent studies revealed...
Ulcerative colitis (UC) is a chronic inflammatory large bowel disease characterized by immune cell infiltration and continuous erosion of intestinal crypts, causing severe ulceration abdominal pain. In the etrolizumab Phase 3 studies, transcriptional analyses colonic biopsies revealed reductions in genes associated with aEb7+ intraepithelial lymphocytes but not adalimumab. Both treatments significantly reduced stromal myeloid cell-associated genes, changes MCS remission status. Generation...
The utility of spatial omics in leveraging cellular interactions normal and diseased states for precision medicine is hampered by a lack strategies matching disease with heterogeneity-guided annotations. Here we use context-dependent approach that matches pattern detection to cell annotation. Using this existing datasets from ulcerative colitis patient colonic biopsies, identified architectural complexities associated difficult-to-detect rare types germinal-center B follicles. Our deepens...
Osteosarcoma is the most common malignant primary bone tumor. The prognosis for patients with disseminated disease remains very poor despite recent advancements in chemotherapy. Moreover, current treatment regimens bear a significant risk of serious side effects. Thus, there an unmet clinical need effective therapies improved safety profiles. Taurolidine antibacterial agent that has been shown to induce cell death different types cancer lines.
Gouty arthritis is caused by the deposition of monosodium urate (MSU) crystals in joints. Despite many treatment options for gout, there a substantial need alternative treatments patients unresponsive to current therapies. Tyrosine kinase inhibitors have demonstrated therapeutic benefit experimental models antibody-dependent and rheumatoid humans, but date, potential effects such on gouty has not been evaluated. Here we demonstrate that with tyrosine inhibitor imatinib mesylate (imatinib)...