A5050032144- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Pancreatic function and diabetes
- Child Nutrition and Water Access
- Iron Metabolism and Disorders
- Fibroblast Growth Factor Research
- Fatty Acid Research and Health
- Trace Elements in Health
- Autophagy in Disease and Therapy
Technical University of Munich
2018-2024
Fresenius (Germany)
2018-2022
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2018-2022
New York University Press
2022
Cambridge University Press
2022
Lipid species patterns are conserved within cells to maintain physicochemical properties of membranes and cellular functions. We present the lipidome, including sterols, glycerolipids (GLs), glycerophospholipids (GPLs), sphingolipids (SLs), primary ex vivo differentiated (I) white, (II) brite, (III) brown adipocytes derived from preadipocytes isolated epididymal inguinal intrascapular adipose tissue. Quantitative lipidomics revealed significantly decreased fractions phosphatidylcholine (PC)...
Studying brown and brite adipose tissue requires precise reliable quantification of cellular thermogenesis. This protocol describes the isolation primary murine pre-adipocytes, differentiation into thermogenic adipocytes, subsequent oxygen consumption analysis. Commonly applied procedures only measure basal maximal proton leak-linked but not explicitly uncoupling protein 1 (UCP1)-dependent respiration. Meaningful analyses require (1) activation UCP1, (2) control over intracellular...
Brown adipocytes are highly specialized cells with the unique metabolic ability to dissipate chemical energy in form of heat. We determined and inferred flux a number key catabolic metabolites, their changes response adrenergic stimulation, dependency on presence thermogenic uncoupling protein 1 and/or oxidative phosphorylation. This study provides reference values approximate rates from limited set measured parameters future thereby allows evaluate plausibility claims about capacity...
Brown adipocytes play a key role in maintaining body temperature as well glucose and lipid homeostasis. However, brown need to adapt their thermogenic activity substrate utilization changes nutrient availability. Amongst the multiple factors influencing adipocyte activity, autophagy is an important regulatory element of capacity activity. Nevertheless, specific sensing mechanism extracellular amino acid availability linking unknown. To characterize transporter PAT2/SLC36A2 adipocytes, loss...
Lipid composition is conserved within sub-cellular compartments to maintain cell function. Lipidomic analyses of liver, muscle, white and brown adipose tissue (BAT) mitochondria revealed substantial differences in their glycerophospholipid (GPL) free cholesterol (FC) contents. The GPL FC ratio was 50-fold higher than mitochondria. Their purity verified by comparison proteomes with ER mitochondria-associated membranes. A lipid signature containing PC FC, calculated from the lipidomic...
High uncoupling protein 1 (Ucp1) expression is a characteristic of differentiated brown adipocytes and linked to adipogenic differentiation. Paracrine fibroblast growth factor 8b (FGF8b) strongly induces Ucp1 transcription in white independent adipogenesis. Here, we report that FGF8b other paracrine FGFs act on preadipocytes upregulate via FGFR1-MEK1/2-ERK1/2 axis, Transcriptomic analysis revealed an upregulation prostaglandin biosynthesis glycolysis upon Fgf8b treatment preadipocytes....
Abstract This study compared the Zn response in selected tissues of weaned piglets fed L-glutamic acid, N,N-diacetic acid (GLDA), while challenged with short-term subclinical deficiency (SZD). During a total experimental period eight days, 96 were restrictively (450 g/d) high phytate (9 g/kg) diet containing added at 0, 5, 10, 15, 20, 25, 45 and 75 mg/kg without 200 GLDA. No animals showed signs clinical no phenotypical differences observed. Broken line analysis status parameters such as...