A5042480115- Diet and metabolism studies
- Gut microbiota and health
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Fatty Acid Research and Health
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Drug Transport and Resistance Mechanisms
- Sphingolipid Metabolism and Signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Eicosanoids and Hypertension Pharmacology
- Metabolism and Genetic Disorders
- Clinical Nutrition and Gastroenterology
- Metabolism, Diabetes, and Cancer
- Immune Response and Inflammation
- Muscle metabolism and nutrition
- Ferroptosis and cancer prognosis
- Clostridium difficile and Clostridium perfringens research
- Dietary Effects on Health
- Genetic factors in colorectal cancer
- Mitochondrial Function and Pathology
University Hospital Regensburg
2011-2024
Technical University of Munich
2015-2024
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2018-2023
Weihenstephan-Triesdorf University of Applied Sciences
2018
Nutritional Research Foundation
2018
University of Regensburg
2006-2014
Analytisches Laboratorium
2011-2012
University of Hawaiʻi at Mānoa
2010
Universitätsklinikum des Saarlandes
2010
University of California, San Francisco
1997
Abstract Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to physiology metabolic diseases. We present a comprehensive multi-omics view of effect intestinal colonization on hepatic metabolism, integrating transcriptomic, proteomic, phosphoproteomic, lipidomic analyses liver plasma samples from germfree specific pathogen-free mice. Microbes induce monounsaturated fatty acid generation by stearoyl-CoA desaturase 1 polyunsaturated elongation...
As the gut microbiota contributes to metabolic health, it is important determine specific diet-microbiota interactions that influence host metabolism. Bile acids and dietary fat source can alter phenotypes of diet-induced obesity, but interplay with intestinal microorganisms unclear. Here, we investigated consequences diets enriched in primary bile or without addition lard palm oil, studied structure functions mice. In combination acids, fed male C57BL/6N mice for a period 8 weeks enhanced...
Extibacter muris is a newly described mouse gut bacterium which metabolizes cholic acid (CA) to deoxycholic (DCA) via 7α-dehydroxylation. Although bile acids influence metabolic and inflammatory responses, few in vivo models exist for studying their metabolism impact on the host. Mice were colonized from birth with simplified community Oligo-MM12 or without E. muris. As of known affect lipid homeostasis, mice fed either low- high-fat diet eight weeks before sampling analyses targeting liver....
Monocytes are precursors of macrophages. Here we demonstrate that macrophage colony-stimulating factor (M-CSF)-dependent differentiation primary human monocytes from healthy volunteers induces transcription SREBP-1c target genes required for fatty acid (FA) biosynthesis and impairs SREBP-2 cholesterol synthesis. Detailed lipid metabolic profiling showed this transcriptional regulation leads to a dramatically increased synthesis as driving force enhanced phospholipid During cell the major...
Species of the genus Wolffia are traditionally used as human food in some Asian countries. All eleven species this genus, identified by molecular barcoding, were investigated for ingredients relevant to nutrition. The total protein content varied between 20 and 30% freeze-dry weight, starch 10 20%, fat 1 5%, fiber was approximately 25%. essential amino acid higher or close requirements preschool-aged children according standards World Health Organization. low, but fraction polyunsaturated...
Sphingolipids comprise a highly diverse and complex class of molecules that serve not only as structural components membranes but also signaling molecules. To understand the differential role sphingolipids in regulatory network, it is important to use specific quantitative methods. We developed novel LC-MS/MS method for rapid, simultaneous quantification sphingolipid metabolites, including sphingosine, sphinganine, phyto-sphingosine, di- trimethyl-sphingosine, sphingosylphosphorylcholine,...
Acylcarnitines are intermediates of fatty acid and amino oxidation found in tissues body fluids. They important diagnostic markers for inherited diseases peroxisomal mitochondrial processes were recently described as biomarkers complex like the metabolic syndrome. Quantification acylcarnitine species can become challenging because various occur isomers and/or have very low concentrations. Here we describe a new LC-MS/MS method quantification 56 with acyl-chain lengths from C2 to C18. Our...
Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) gut microbiota relevant for microbe⁻host communication. Here, we present a method quantification SCFAs in fecal samples liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The includes acetate, propionate, butyrate, isobutyrate with run time 4 min. reproducible (coefficients variation (CV) below 10%)...
Diurnal regulation of whole-body lipid metabolism plays a vital role in metabolic health. Although changes levels across the diurnal cycle have been investigated, system-wide molecular responses to both short-acting fasting-feeding transitions and longer-timescale circadian rhythms not explored parallel. Here, we perform time-series multi-omics analyses liver plasma revealing that majority oscillations are entrained by adaptations fasting, food intake, postprandial state. By developing...
The mechanisms that maintain relative uterine quiescence during pregnancy remain largely unknown. A possible role for nitric oxide has recently emerged, however, the expression of synthase within human myometrium at midgestation, a time when uterus is normally quiescent, not been investigated. purpose this study was to identify cell types in contain inducible (iNOS), and examine changes its labor. We found iNOS expressed smooth muscle cells pregnant myometrium. Expression highest preterm...
ATP-binding cassette (ABC) transporters regulate the transport of a variety physiologic substrates. Moreover, several human ABC proteins are responsible for drug exclusion in compound-treated tumor cells, providing cellular mechanisms development multidrug resistance and, therefore, playing an important role malignant transformation. As only limited information exists on melanoma, aim study was to generate complete expression profile this entity. Using TaqMan low-density array 47...
Eicosanoids are key mediators and regulators of inflammation oxidative stress often used as biomarkers for diseases pathological conditions such cardiovascular pulmonary cancer. Analytically, comprehensive robust quantification different eicosanoid species in a multi-method approach is problematic because most these compounds relatively unstable may differ their chemical properties. Here we describe novel ultra-performance liquid chromatography-selected reaction monitoring mass spectroscopy...
Lipid species patterns are conserved within cells to maintain physicochemical properties of membranes and cellular functions. We present the lipidome, including sterols, glycerolipids (GLs), glycerophospholipids (GPLs), sphingolipids (SLs), primary ex vivo differentiated (I) white, (II) brite, (III) brown adipocytes derived from preadipocytes isolated epididymal inguinal intrascapular adipose tissue. Quantitative lipidomics revealed significantly decreased fractions phosphatidylcholine (PC)...
Gut microbiota significantly influence the plasma and liver lipidome. An interconnecting metabolite is acetate generated after degradation fermentation of dietary fiber by gut microbiota, which metabolized in into longer chain fatty acids complex lipids reaching circulation. Whether these systemic changes are accompanied alternations intestinal lipidome unclear. Therefore, we quantified glycerophospholipids, sphingolipids sterols ileum colon, two segments containing highest densities...
Abstract Recent studies indicate that adipose tissue in obesity promotes breast cancer progression by secreting protumorigenic chemokines, growth factors, and fatty acids. However, the detailed mechanisms which hypertrophic influences cells are still not well understood. Here we show co-culture with from high-fat diet induced obese C57BL/6 mice alters transcriptome profiles triple-negative (TNBC) cells, leading to upregulation of genes involved inflammation lipid metabolism, such as IL1B,...