A5100612372- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Phytochemistry and Biological Activities
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- RNA modifications and cancer
- Synthesis and biological activity
- CRISPR and Genetic Engineering
- Angiogenesis and VEGF in Cancer
- Natural product bioactivities and synthesis
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Drug Transport and Resistance Mechanisms
- Phytochemical Studies and Bioactivities
- Ferrocene Chemistry and Applications
- Cancer-related molecular mechanisms research
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
Beijing University of Chemical Technology
2024
Quantum (Australia)
2023
Huaqiao University
2013-2023
Ministry of Education of the People's Republic of China
2009-2015
Institute of Molecular Medicine
2008-2014
University of Pennsylvania
2010
University of Hong Kong
2003-2008
Quanzhou Normal University
2008
Harbin Medical University
2008
Chinese Academy of Medical Sciences & Peking Union Medical College
2005-2006
The brain is generally considered immunoprivileged, although increasing examples of immunological responses to antigens, neuronal expression major histocompatibility class I genes, and neurological autoimmunity have been recognized. An adeno-associated virus (AAV) vaccine generated autoantibodies that targeted a specific protein, the NR1 subunit N -methyl- d -aspartate (NMDA) receptor. After peroral administration AAV vaccine, transgene persisted for at least 5 months was associated with...
Fabry disease is a lysosomal storage disorder caused by deficiency of the enzyme α-galactosidase A (α-gal A). This leads to impaired catabolism α-galactosyl-terminal lipids such as globotriaosylceramide (Gb3). Patients develop painful neuropathy and vascular occlusions that progressively lead cardiovascular, cerebrovascular, renal dysfunction early death. Although replacement therapy bone marrow transplantation have shown promise in murine analog disease, gene holds strong potential for...
Abstract Recombinant adeno-associated virus 2/5 (rAAV2/5), a hybrid rAAV-2 with AAV-5 capsid, seems to be very efficient delivery vector for the transduction of lung adenocarcinoma cell line A549. Infection A549 rAAV2/5 encoding extracellular domain tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, amino acids 114-281) resulted in secretion soluble TRAIL (sTRAIL) and induction apoptosis these cells. rAAV2/5-sTRAIL mediated stable expression sTRAIL presence trimeric form sera...
Overoxidized polypyrrole/graphene modified glassy carbon electrode (PPyox/graphene/GCE) has been prepared and applied for the fabrication of dopamine sensors without interference ascorbic acid. Compared to bare GCE, a substantial negative shift anodic peak potential dramatic increase current signal were observed separations between cathodic peaks was obviously decreased, demonstrating that PPyox/graphene/GCE exhibited favorable electron transfer kinetics electrocatalytic activity towards...
Prostate cancer (PCa) is a major health problem in males. Metastasis-associated with lung adenocarcinoma transcript-1 (MALAT1), which overexpressed PCa tissue, associated physiological and pathological conditions of PCa. M2 macrophages are immune cells abundant the tumor microenvironment. However, it remains unknown whether involved effects or not, molecular mechanisms MALAT1 on progression have not yet been comprehensively explored. Here we reported that, (PMA/IL-4 treated THP1) induced...
Recombinant adeno-associated viruses (rAAV) are highly efficient vectors for gene transfer into the central nervous system (CNS). However, a major hurdle delivery to mammalian brain is achieve high-level transduction in target cells beyond immediate injection site. Therefore, addition improvements expression cassettes and viral titers, optimal parameters need be defined. Here, we show that previous studies of somatic cell have used suboptimal parameters, with even lowest reported perfusion...
Cytoglobin (Cygb), a member of the hexacoordinate globin superfamily (hxHb), is expressed in fibroblasts from broad range tissues. The physiological functions hxHb are still unclear, but biochemical studies reveal that they can scavenge toxic species, such as nitric oxide, peroxynitrite, and hydrogen peroxide. We demonstrate overexpression Cygb rat hepatic stellate cells, both vitro vivo, protects against oxidative stress, inhibiting their differentiation into myofibroblast-like phenotype....
Lung cancer, the leading cause of mortality in both men and women United States, is largely diagnosed at its advanced stages that there are no effective therapeutic alternatives. Although tobacco smoking well established lung underlying mechanism for tumorigenesis remains poorly understood. An important event tumor development appears to be epigenetic alterations, especially change DNA methylation patterns, which induce most suppressor gene silence. In one scenario, methyltransferase (DNMT)...
Transcatheter arterial embolization (TAE) is a well-established technique for unresectable hepatic malignancies. However, TAE can temporally halt the growth of tumors by blocking their supply, but often rapidly develop collateral blood vessels via angiogenesis. We have previously demonstrated that intraportal delivery adeno-associated viral particles expressing angiostatin leads to long-term expression capable inhibiting angiogenesis and suppressing outgrowth in liver. Thus, we hypothesize...
Abstract Purpose: Angiogenesis inhibitors have strong therapeutic potential as antitumor agents in suppressing tumor growth and metastatic progression. Vasostatin, the N-terminal domain of calreticulin, is a potent angiogenesis inhibitor. In this study, we determined effectiveness vasostatin delivered by recombinant pseudotype adeno-associated virus 2/5 (rAAV2/5-VAS) gene therapy approach for lung cancer treatment. Experimental Design: We used rAAV2/5 to deliver intratumorally or...
Leaves of Murraya exotica L. (Rutaceae) are used for the treatment various disorders such as cough, fever, and infectious wounds, well alleviating pains in folk medicine southern China. The objectives this study were to investigate vivo antinociceptive anti-inflammatory activities ethanol (70%) extracts isolated compounds obtained from dried leaves M. exotica. evaluated with methods acetic acid-induced writhing response hot-plate latent pain test. Carrageenan induced hind paw edema, xylene...
Kallistatin, which protects organs and cells against inflammation, fibrosis oxidative stress, is mainly synthesized secreted in liver. However, its relationship to human liver disease remains unclear. The purpose of this study was explore the between serum kallistatin clinical evidence both cirrhosis hepatocellular carcinoma (HCC), determine if levels could be used as a diagnostic indicator hepatic health status, especially (LC). Our cohort consisted 115 patients with clinically proven (LF),...
Liver fibrosis is the consequence of activation hepatic stellate cells mediated by persistent or recurrent liver injury, where oxidative stress inflammatory response resulting from immune and cytokines are involved. Targeting could be an important strategy for therapy fibrosis. In this study, we showed a tropism recombinant adeno-associated virus (rAAV, serotype 2) with high efficiency in transduction homeostatic gene, heme oxygenase-1 (HO-1), to activated cells. The binding rAAVs increased...