A5100692564- Computational Drug Discovery Methods
- Ion Channels and Receptors
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Asymmetric Hydrogenation and Catalysis
- Flexible and Reconfigurable Manufacturing Systems
- Metabolomics and Mass Spectrometry Studies
- Organometallic Complex Synthesis and Catalysis
- Diabetes Treatment and Management
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Particle physics theoretical and experimental studies
- Analytical Chemistry and Chromatography
- Pharmacological Effects of Natural Compounds
- Diabetes Management and Research
- Neurobiology and Insect Physiology Research
- Pediatric health and respiratory diseases
- Complementary and Alternative Medicine Studies
- Quantum Chromodynamics and Particle Interactions
- Reformation and Early Modern Christianity
- Statistical and Computational Modeling
- Advanced Chemical Physics Studies
Freie Universität Berlin
1973-2024
Max Planck Institute for Terrestrial Microbiology
2024
Bayer (Germany)
2005-2024
Klinikum Magdeburg
2024
Jena University Hospital
2017-2024
Universitätsmedizin Greifswald
2003-2024
University of Johannesburg
2024
University of Zurich
2024
Humboldt-Universität zu Berlin
2015-2024
Charité - Universitätsmedizin Berlin
2005-2024
Treatment with elexacaftor/tezacaftor/ivacaftor (ETI) improves multiple clinical outcomes in people cystic fibrosis (pwCF) at least one F508del allele. This study evaluated the real-world impact of ETI on lung function, nutritional status, pulmonary exacerbation frequency, and sweat chloride concentrations a large group pwCF.This observational cohort used data from German CF Registry for pwCF who received therapy were followed up period 12 months.The included 2645 67 centres Germany (mean...
Ca2+ signaling includes store-operated entry (SOCE) following depletion of endoplas-mic reticulum (ER) stores. On store depletion, the ER sensor STIM1 activates Orai1, pore-forming unit Ca2+-release-activated (CRAC) channels. Here, we show that Orai1 is regulated by serum- and glucocorticoid-inducible kinase 1 (SGK1), a growth factor-regulated kinase. Membrane protein abundance, /CRAC, SOCE in human embryonic kidney (HEK293) cells stably expressing transfected with were each significantly...
// Sebastian Schmidt 1 , Guoxing Liu Guilai Wenting Yang Sabina Honisch Stavros Pantelakos 1,2 Christos Stournaras Arnd Hönig 3 Florian Lang Department of Physiology, University Tübingen, D72076 Germany 2 Crete Medical School, Biochemistry, Voutes, Heraklion, 71110, Greece Gynecology, Hospital Würzburg, D97080 Correspondence: Lang, email: Keywords : Ca2+ release activated channel, SOCE, Akt, SH-6, 2-APB, apoptosis Received April 22, 2014 Accepted May 28, Published Abstract Mechanisms...
The serum and glucocorticoid-inducible kinase SGK1 increases the activity of Orai1, pore forming unit store-operated Ca(2+) entry, thus influences Ca(2+)-dependent cellular functions such as migration. further regulates transcription factor nuclear κB (NF-κB). This study explored whether Orai1 and/or STIM1, Orai1-activating sensor. STIM1 transcript levels were decreased in mast cells from knock-out mice increased HEK293 transfected with active (S422D)SGK1 but not inactive (K127N)SGK1 or...
Comprehensive multiphase (CMP) NMR is a novel technology that integrates all the hardware from solution-, gel- and solid-state into single probe, permitting phases to be studied in intact samples. Here comprehensive used study components living organism for first time. This work describes 4 new scientific accomplishments summarized as: (1) CMP-NMR applied animal, (2) an effective method deliver oxygen organisms described which permits longer studies essential in-depth analysis general, (3)...
Platelet activation is essential for primary hemostasis and acute thrombotic vascular occlusions. On activation, platelets release their prothrombotic granules expose phosphatidylserine, thus fostering thrombin generation thrombus formation. In other cell types, both degranulation phosphatidylserine exposure are modified by sphingomyelinase-dependent formation of ceramide. The present study explored whether acid sphingomyelinase participates in the regulation platelet secretion, exposure,...
Explainable artificial intelligence (XAI) methods have shown increasing applicability in chemistry. In this context, visualization techniques can highlight regions of a molecule to reveal their influence over predicted property. For purpose, some XAI calculate attribution scores associated with tokens SMILES strings or atoms molecule. While an association score atom be directly visually represented on diagram, computed for non-atom cannot. instance, substring [N+] contains 3 tokens, i.e., [,...
A symmetry-preserving continuum approach to the two valence-body bound-state problem is used calculate valence, glue and sea distributions within pion; unifying them with, inter alia, electromagnetic pion elastic transition form factors. The analysis reveals following momentum fractions at scale $\zeta_2:=2\,$GeV: $\langle x_{\rm valence} \rangle = 0.48(3)$, glue} 0.41(2)$, sea} 0.11(2)$; despite hardening induced by emergent phenomenon of dynamical chiral symmetry breaking, valence-quark...
Conceptually, radii are amongst the simplest Poincar\'e-invariant properties that can be associated with hadrons and light nuclei. Accurate values of these quantities necessary so one may judge character putative solutions to strong interaction problem within Standard Model. However, limiting their ability serve in this role, recent measurements new analyses older data have revealed uncertainties imprecisions proton, pion, kaon, deuteron. In context radius measurement using electron + hadron...
Abstract enviPath is a widely used database and prediction system for microbial biotransformation pathways of primarily xenobiotic compounds. Data are freely available both via web interface public REST API. Since its initial release in 2016, we extended the data improved performance usability overall system. We now provide three diverse sets, covering different environments under experimental conditions. This also enabled developing pathway model that applicable to more set chemicals. In...
The catalytic cycle of the Rh-catalyzed monohydroformylation 1,3-butadiene with a triptycene-derived bisphosphite ligand was investigated density functional theory, as it determines selectivity 1,4-bis-hydroformylation to adipic aldehyde, dream reaction chemical industry. Out variety possible reactive pathways, two dominant ones were highlighted leading monoaldehydes 3-pentenal and 4-pentenal, which experimentally also are main primary products. With catalysts like one studied here, highly...
Platelets are activated by increased cytosolic Ca2+ concentration ([Ca2+]i) following store-operated calcium entry (SOCE) accomplished calcium-release-activated (CRAC) channel moiety Orai1 and its regulator STIM1. In other cells, transport is regulated 1,25(OH)2 vitamin D3 [1,25(OH)2D3]. 1,25(OH)2D3 formation inhibited klotho excessive in klotho-deficient mice (kl/kl). The present study explored the effect of deficiency on platelet signaling activation. megakaryocytes isolated from WT...
Protein crystallization offers great potential in downstream processing of pharmaceutical protein active ingredients. The advantages, which are well known and widely utilized low-molecular weight crystallization, can also be expected to found some extent crystallization. However, there is still a marked need for improvement two main areas processing, namely, from impure solutions scale-up.
Abstract Avoidance and elimination of viral contamination in biotechnological pharmaceutical processes is a crucial issue. The novel UVivatec coiled tube reactor was designed for UV irradiation cloudy liquid media. It very effectively inactivates especially critical viruses avoids high product losses. method proposed an ideal complement to existing established processes.
The hydroformylation catalyst (L)RhH(CO)2, with a triptycene-derived bisphosphite ligand, and its iridium analogue were studied in solution, the solid state, computationally (DFT) order to determine their molecular structures understand equilibria between isomers. predicted intramolecular distance of two biphenyl side groups these ligands strongly depends upon treatment inter- noncovalent interactions. A balanced "weak" interactions is prerequisite for obtaining reasonable consequently also...
Functional membrane androgen receptors (mAR) have previously been described in MCF-7 breast cancer cells. Their stimulation by specific testosterone albumin conjugates (TAC) activate rapidly non-genomic FAK/PI3K/Rac1/Cdc42 signaling, trigger actin reorganization and inhibit cell motility. PI3K stimulates serum glucocorticoid inducible kinase SGK1, which turn regulates the function of mAR. In present study we addressed role SGK1 mAR-induced apoptosis. TAC-stimulated mAR activation elicited...
Background: Chorein, a protein encoded by VPS13A (vacuolar sorting-associated 13A), is defective in chorea acanthocytosis, rare disease characterized acanthocytosis of red blood cells and neuronal cell death with progressive hyperkinetic movement disorder, cognitive dysfunction, behavioral abnormalities chronic hyperkalemia. Chorein highly expressed ZF rhabdomyosarcoma counteracts apoptosis those cells. effective part interacting fostering stimulation phosphoinositide-3-kinase...
Membrane androgen receptors (mAR) are expressed in several tumors. mAR activation by testosterone albumin conjugates (TAC) suppresses tumor growth and migration. signaling involves phosphoinositide-3-kinase (PI3K) Rho-associated protein kinase (ROCK). PI3K stimulates serum- glucocorticoid-inducible SGK1, which turn activates Na(+)/H(+)-exchangers (NHE). In prostate cancer cells cytosolic pH (pHi) was determined utilizing 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-fluorescence...
Abstract What should we believe when epistemic and practical reasons pull in opposite directions? The traditional view states that there is something ought epistemically to practically (cause ourselves to) believe, period. More recent accounts challenge this view, either by arguing simpliciter all considered (the weighing view), or denying the normativity of altogether (epistemic anti‐normativism). I argue against both defend view. An agent can be blameworthy doxastic dilemmas for complying...