A5019079382- Ichthyology and Marine Biology
- Developmental Biology and Gene Regulation
- dental development and anomalies
- Paleontology and Evolutionary Biology
- Bone and Dental Protein Studies
- Oral and Maxillofacial Pathology
- Marine animal studies overview
- Bone Metabolism and Diseases
- Fish Ecology and Management Studies
- Congenital heart defects research
- Animal Genetics and Reproduction
- Fish Biology and Ecology Studies
- Identification and Quantification in Food
- Fish biology, ecology, and behavior
- Genomics and Phylogenetic Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Zebrafish Biomedical Research Applications
- Forensic Anthropology and Bioarchaeology Studies
- Reproductive biology and impacts on aquatic species
- Morphological variations and asymmetry
- Effects and risks of endocrine disrupting chemicals
- Proteoglycans and glycosaminoglycans research
- Comparative Animal Anatomy Studies
Institut des Sciences de l'Evolution de Montpellier
2016-2025
Université de Montpellier
2014-2025
Institut de Recherche pour le Développement
2015-2025
Centre National de la Recherche Scientifique
2010-2025
École Pratique des Hautes Études
2015-2024
University of Ottawa
2010-2013
Génétique Quantitative et Évolution Le Moulon
2006-2013
Ontario Genomics
2010-2011
Université Paris-Sud
2008-2011
Évolution, Génomes, Comportement, Écologie
2007-2010
Abstract Background The gene regulatory network involved in tooth morphogenesis has been extremely well described mammals and its modeling allowed predictions of variations pathway that may have led to evolution shapes. However, very little is known outside understand how this framework also account for shape at the level gnathostomes. In work, we describe expression patterns proliferation/apoptosis assays uncover homologous pathways catshark Scyliorhinus canicula . Results Because their...
SUMMARY β-Adrenergic receptors (βARs) are crucial for maintaining the rate and force of cardiac muscle contraction in vertebrates. Zebrafish (Danio rerio) have one β1AR gene two β2AR genes (β2aAR β2bAR). We examined roles these larval zebrafish vivo by assessing impact translational knockdown on function. larvae lacking expression morpholino displayed lower heart rates than control fish, whereas deficient both β2aAR β2bAR exhibited significantly higher controls. These results suggested a...
Abstract Background Teeth and tooth-like structures, together named odontodes, are repeated organs thought to share a common evolutionary origin. These structures can be found in gnathostomes at different locations along the body: oral teeth jaws, denticles oral-pharyngeal cavity, dermal on elasmobranch skin. We, other colleagues, had previously shown that any location were serially homologous because: i) pharyngeal develop through developmental module; ii) expression patterns of Dlx genes...
SUMMARY In order to investigate similarities and differences in genetic control of development among teeth within between species, we determined the expression pattern all eight Dlx genes zebrafish during pharyngeal dentition compared these data with that reported for mouse molar tooth development. We found (i) dlx1a dlx6a are not expressed teeth, contrast their murine orthologs, Dlx1 Dlx6 ; (ii) six other overlaps time space, particularly early morphogenesis; (iii) different locations...
Understanding the evolutionary emergence and subsequent diversification of vertebrate skeleton requires a comprehensive view diverse skeletal cell types found in distinct developmental contexts, tissues, species. To date, our knowledge molecular nature shark calcified extracellular matrix, its relationships with osteichthyan remain scarce. Here, based on specific combinations expression patterns Col1a1, Col1a2, Col2a1 fibrillar collagen genes, we compare footprint endoskeletal elements from...
The mitogenome is an inescapable tool in conservation biology studies. Yet, its routine sequencing may remain tricky despite next-generation technologies. An enrichment step often necessary but not always straightforward depending on the initial DNA quality or quantity. Furthermore, availability of close mitochondrial reference sequences for non-model species limits primer design long-range PCR bait synthesis. Here we propose easy and cost-effective protocol without building multiplexed...
Abstract Small Leucine-Rich Proteoglycans (SLRPs) are a major family of vertebrate proteoglycans. In bony vertebrates, SLRPs have variety functions from structural to signaling and found in extracellular matrices, notably skeletal tissues. However, there is little or no data on the diversity, function expression patterns cartilaginous fishes, which hinders our understanding how these genes evolved with diversification particular regarding early events whole genome duplications that shaped...
It is now well established that there were four Hox gene clusters in the genome of last common ancestor extant gnathostomes. To better understand evolution organization and expression these genomic regions, we have studied a shark (Scyliorhinus canicula). We sequenced 225,580 expressed sequence tags from several embryonic cDNA libraries. Blast searches identified corresponding transcripts to almost all HoxA, HoxB, HoxD cluster genes. No HoxC transcript was identified, suggesting this absent...
Abstract The Dlx genes encode a family of transcription factors important for the development vertebrate forebrain. These have very similar expression domains during telencephalon in mice and play role γ‐aminobutyric acid (GABAergic) interneuron differentiation. We used triple fluorescent situ hybridization to study relative dlx gad1 zebrafish diencephalon. also generated transgenic with regulatory elements from dlx1a/2a locus. recapitulated forebrain mimicked relationship between . Finally,...
SUMMARY The Hox gene family encodes homeodomain-containing transcription factors involved in the patterning of structures composed repeated elements along antero-posterior axis Bilateralia embryos. In vertebrate, genes are thought to control segmental identity rhombomeres, branchial arches, and somites. They therefore have played a key role morphological evolution like jaw, girdles, vertebrae gnathostomes. Thus far, our knowledge about expression patterns genes, code, has been mainly...
Abstract In order to characterize the molecular bases of mineralizing cell evolution, we targeted type X collagen, a nonfibrillar network forming collagen encoded by Col10a1 gene. It is involved in process endochondral ossification ray-finned fishes and tetrapods (Osteichthyes), but until now unknown cartilaginous (Chondrichthyes). We show that holocephalans elasmobranchs have respectively five six tandemly duplicated gene copies display conserved genomic synteny with osteichthyan genes. All...
Domestic animals are often described as paedomorphic, meaning that they retain juvenile characteristics into adulthood. Through a three-dimensional landmark-based geometric morphometric analysis of cranial morphology at three growth stages, we demonstrate wild boar (n = 138) and domestic pigs 106) (Sus scrofa) follow distinct ontogenetic trajectories. With the exception size ratio between facial neurocranial regions, paedomorphism does not appear to be primary pattern describing observed...
The diversity of skeletal tissues in extant vertebrates includes mineralized and unmineralized structures made bone, cartilage, or intermediate nature. This variability, together with the diverse nature fossil species question origin skeletonization early vertebrates. In particular, study cartilaginous fishes is currently mostly restrained to tessellated a derived form cartilage that evolved at this group. work, we describe architectural histological neural arch mineralization fishes....
Developmental complexity stemming from the dynamic interplay between genetic and biomechanic factors canalizes ways genotypes phenotypes can change in evolution. As a paradigmatic system, we explore how changes developmental generate typical tooth shape transitions. Since development has mainly been researched mammals, contribute to more general understanding by studying of diversity sharks. To this end, build general, but realistic, mathematical model odontogenesis. We show that it...
Abstract Gnathostome teeth are one of the most promising models for developmental evolutionary studies, they abundant organ in fossil record and an excellent example organogenesis. Teeth have a complex morphology restricted to mouth mammals, whereas actinopterygian simple found several locations, notably on pharyngeal bones. Morphological similarities support hypothesis that oral serially homologous. Gene expression data from mouse some teleosts shown gene families involved odontogenesis...
One of the goals evolutionary developmental biology is to link specific adaptations changes in pathways. The dentition cypriniform fishes, which contrast many other teleost fish species possess pharyngeal teeth but lack oral teeth, provides a suitable model study development feeding adaptations. Here, we have examined involvement retinoic acid (RA) tooth and show that RA specifically required induce program zebrafish. Perturbation signaling at this stage abolished induction without affecting...
Abstract During vertebrate forebrain formation, Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes γ‐aminobutyric acid (GABA)‐expressing neurons, including inhibitory cortical interneurons mammals. They also participate regulation Gad encoding enzymes necessary for GABA synthesis. In mice, at least four cis ‐regulatory elements (CREs) control expression telencephalon diencephalon:...
Abstract We have used dlx genes to test the hypothesis of a separate developmental program for dermal and cartilage bones within neuro‐ splanchnocranium by comparing expression patterns all eight during cranial bone formation in zebrafish from 1 day postfertilization (dPF) 15 dPF. are expressed visceral skeleton but not or braincase. The spatiotemporal pattern members gene family, support view that impart cellular identity different arches, required make arch‐specific bones. Expression...
The molecular bases explaining the diversity of dental tissue mineralization across gnathostomes are still poorly understood. Odontodes, such as teeth and body denticles, serial structures that develop through deployment a gene regulatory network shared between all gnathostomes. Dentin, inner odontode mineralized tissue, is produced by odontoblasts appears well-conserved evolution. In contrast, hypermineralized external layer (enamel or enameloid) ameloblasts epithelial origin, shows...
Abstract In bony vertebrates, skeletal mineralization relies on the secretory calcium-binding phosphoproteins (Scpp) family whose members are acidic extracellular proteins posttranslationally regulated by Fam20°C kinase. As scpp genes absent from elephant shark genome, they currently thought to be specific fishes (osteichthyans). Here, we report a gene present in elasmobranchs (sharks and rays) that evolved local tandem duplication of sparc-L 5′ exons show both experienced recent conversion...
Abstract Cartilaginous fishes (chondrichthyans: chimaeras and elasmobranchs -sharks, skates rays) hold a key phylogenetic position to explore the origin diversifications of jawed vertebrates. Here, we report integrate reference genomic, transcriptomic morphological data in small-spotted catshark Scyliorhinus canicula shed light on evolution sensory organs. We first characterise general aspects genome, confirming high conservation genome organisation across cartilaginous fishes, investigate...
Abstract Teeth in sharks are shed and replaced throughout their lifetime. Morphological dental changes through ontogeny have been identified several species correlated with shifts diet the acquisition of sexual maturity. However, these were rarely quantified detail along multiple ontogenetic stages, which makes it difficult to infer developmental processes responsible for observed plasticity. In this work, we use micro‐computed tomography 3D geometric morphometrics describe analyze tooth...