A5073410181- Zebrafish Biomedical Research Applications
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- dental development and anomalies
- Microplastics and Plastic Pollution
- Effects and risks of endocrine disrupting chemicals
- Iron Metabolism and Disorders
- Aquaculture Nutrition and Growth
- Pancreatic function and diabetes
- Congenital heart defects research
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Medical and Biological Sciences
- Developmental Biology and Gene Regulation
- Reproductive biology and impacts on aquatic species
- Aquaculture disease management and microbiota
- Retinoids in leukemia and cellular processes
- Pregnancy and preeclampsia studies
- Recycling and Waste Management Techniques
- Regulation of Appetite and Obesity
- Liver Disease Diagnosis and Treatment
- Science, Research, and Medicine
- Histone Deacetylase Inhibitors Research
- Hemoglobinopathies and Related Disorders
- Ionic liquids properties and applications
University of Mississippi Medical Center
2019-2024
University of Mississippi
2022-2023
Deakin University
2013-2022
State Street (United States)
2020-2022
Jackson Memorial Hospital
2019-2021
Imperial College London
2016
Australian Centre for Disease Preparedness
2015
Tufts University
2014
Institut de Génomique Fonctionnelle de Lyon
2008-2013
Université Claude Bernard Lyon 1
2008-2011
The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition examined expression key lipid-regulating genes zebrafish embryogenesis. We also conducted a semiquantitative analysis lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, analyzed processing boron-dipyrromethene (BODIPY) analogs injected into the yolk thin layer...
Bisphenol A (BPA) is an endocrine disruptor that displays estrogenic activity. Several reports suggest BPA may have estrogen receptor-independent effects. In zebrafish, 50 μM exposure induces otic vesicle abnormalities, including otolith aggregation. The purpose of this study was to test if action mediated in vivo during zebrafish development by the orphan nuclear related receptor (ERR) γ. Combining pharmacological and functional approaches, we demonstrate ERRγ mediates BPA-induced...
Most marine organisms have a biphasic life cycle during which pelagic larvae transform into radically different juveniles. In vertebrates, the role of thyroid hormones (THs) in triggering this transition is well known, but how morphological and physiological changes are integrated coherent way with ecological remains poorly explored. To gain insight question, we performed an analysis metamorphosis teleost, false clownfish (Amphiprion ocellaris). We show THs coordinate change color vision as...
Abstract Background The plastic monomer and plasticizer bisphenol A (BPA), used for manufacturing polycarbonate epoxy resins, is produced at over 2.5 million metric tons per year. Concerns have been raised that BPA acts as an endocrine disruptor on both developmental reproductive processes a large body of evidence suggests interferes with estrogen thyroid hormone signaling. Here, we investigated effects during embryonic development using the zebrafish Xenopus models. Results We report...
Central and peripheral mechanisms modulate food intake energy balance in mammals the precise role of type 1 cannabinoid receptor (CB1) these processes is still being explored. Using zebrafish, Danio rerio, we show that rimonabant, a CB1-specific antagonist with an EC50 5.15 × 10−8 m, decreases embryonic yolk sac reserve use. We reveal developmental overlap between CART genes CB1 expression hypothalamus medulla oblongata, two brain structures play crucial roles appetite regulation mammals....
Hepcidin, a peptide hormone that decreases intestinal iron absorption and macrophage release, is potential drug target for patients with overload syndromes because its levels are inappropriately low in these individuals. Endogenous stimulants of Hepcidin transcription include bone morphogenic protein 6 (BMP6) interleukin-6 (IL-6) by effects on mothers against decapentaplegic homolog (Smad)4 or signal transducer activator (Stat)3, respectively. We conducted small-scale chemical screen...
According to Dollo’s Law of irreversibility in evolution, a lost structure is usually considered be unable reappear evolution due the accumulation over time mutations genes required for its formation. Cypriniform fish are classic model evolutionary loss because, while they form fully operational teeth ventral posterior pharynx, unlike other teleosts, do not possess oral teeth. Paleontological data show that Cypriniforms, clade teleost includes zebrafish, their 50 100 Mya. In order attempt...
Vertebrate forelimbs arise as bilateral appendages from the lateral plate mesoderm (LPM). Mutants in aldh1a2 (raldh2), an embryonically expressed gene encoding a retinoic acid (RA)-synthesizing enzyme, have been used to show that limb development and patterning of bud are crucially dependent on RA signaling. However, timing cellular origin signaling these processes remained poorly resolved. We genetics chemical modulators resolve issues zebrafish. By rescuing pectoral fin induction...
Background Endocrine disrupting chemicals are widely distributed in the environment and derive from many different human activities or can also be natural products synthesized by plants microorganisms. The phytoestrogen, genistein (4′, 5, 7-trihydroxy-isoflavone), is a naturally occurring compound found soy products. Genistein has been subject of numerous studies because its known estrogenic activity. Methodology/Principal Findings We report that exposure zebrafish embryos induces apoptosis,...
Abstract Histone deacetylase 3 ( Hdac3 ) regulates the expression of lipid metabolism genes in multiple tissues, however its role regulating intestinal epithelium is unknown. Here we demonstrate that intestine-specific deletion IKO protects mice from diet induced obesity. Intestinal epithelial cells (IECs) display co-ordinate induction and proteins involved mitochondrial peroxisomal β-oxidation, have an increased rate fatty acid oxidation, undergo marked remodelling their lipidome,...
Abstract Maternal hyperglycaemia has a profound impact on the developing foetus and increases risk of abnormalities like obesity, impaired glucose tolerance insulin secretory defects in post-natal life. Increased levels blood stream due to diabetes causes visual disorders retinopathy. However, maternal pre-existing or gestational foetal retina is unknown. The aim this work was study effect using zebrafish as vertebrate model. Wild-type transgenic embryos were exposed 0, 4 5% D-Glucose...
Abstract Adiponectin is an adipocytokine that plays important roles in glucose and lipid homeostasis. binds to two types of transmembrane receptors: receptor (AdipoR) type 1 2. We isolated characterized the adiponectin genes ( A B ) three receptors zebrafish. In adult, only detected kidney while mRNAs are widely expressed liver, adipose tissue, muscle, brain. The found many tissues such as brain, gut, kidney, ovary. Interestingly, we detect embryonic synexpression all pharyngeal region....
During vertebrate craniofacial development, neural crest cells (NCCs) contribute to most of the pharyngeal skeleton. Defects in NCC specification, migration and differentiation resulting malformations complex are associated with human disorders including Treacher-Collins Syndrome, caused by mutations TCOF1. It has been hypothesized that perturbed ribosome biogenesis p53 mediated neuroepithelial apoptosis results hypoplasia mouse Tcof1 mutants. However, underlying mechanisms linking...
Background and Aims A major impediment to establishing new treatments for non-alcoholic steatohepatitis is the lack of suitable animal models that accurately mimic biochemical metabolic characteristics disease. The aim this study was explore a unique polygenic model disease as by determining effects 2% dietary cholesterol supplementation on liver endpoints in Psammomys obesus (Israeli sand rat). Methods P. were provided ad libitum access either standard rodent diet (20% kcal/fat) or...
A growing goal in the field of metabolism is to determine impact genetics on different aspects mitochondrial function. Understanding these relationships will help understand underlying etiology for a range diseases linked with dysfunction, such as diabetes and obesity. Recent advances instrumentation, has enabled monitoring distinct parameters function cell lines or tissue explants. Here we present method rapid sensitive analysis vivo during zebrafish embryonic development using Seahorse...
The endocannabinoid system (ECS) and retinoic acid (RA) signaling have been associated with influencing lipid metabolism. We hypothesized that modulation of these pathways could modify abundance in developing vertebrates a combinatorial effect on levels. Zebrafish embryos were exposed to chemical treatments altering the activity ECS RA pathway. Embryos stained neutral dye Oil-Red-O (ORO) underwent whole-mount situ hybridization (WISH). Mouse 3T3-L1 fibroblasts differentiated under exposure...