A5024805908- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Criminal Justice and Penology
- Eosinophilic Esophagitis
- Tryptophan and brain disorders
- Protein purification and stability
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Stress Responses and Cortisol
- Food Allergy and Anaphylaxis Research
- Lysosomal Storage Disorders Research
- Comparative constitutional jurisprudence studies
- Data Privacy and Cybersecurity
- Gut microbiota and health
Polpharma Biologics (Netherlands)
2010-2024
Indoleamine 2,3-dioxygenases (IDOs) degrade l-tryptophan to kynurenines and drive the de novo synthesis of nicotinamide adenine dinucleotide. Unsurprisingly, various invertebrates, vertebrates, even fungi produce IDO. In mammals, IDO1 also serves as a homeostatic regulator, modulating immune response infection via local tryptophan deprivation, active catabolite production, non-enzymatic cell signaling. Whether fungal Idos have pleiotropic functions that impact on host-fungal physiology is...
Abstract Therapeutic monoclonal antibodies, a highly successful class of biological drugs, are conventionally manufactured in mammalian cell lines. A recent approach to increase the therapeutic effectiveness antibodies has been combine two or more them; however this increases complexity development and manufacture. To address issue method efficiently express multiple from single developed we describe here generation stable clones that high levels human antibody mixture. PER.C6® cells were...
Background Cow's milk-derived whey hydrolysates are nutritional substitutes for allergic infants. Safety or residual allergenicity assessment of these is crucial. Currently, rat basophilic leukemia RBL-2H3 cells expressing the human IgE receptor α-chain (huFcεRIα-RBL-2H3), sensitized with serum from cow's milk children, being employed to assess in vitro hydrolysates. However, limited availability and inter-lot variation sera impede standardization hydrolysate safety testing degranulation...
Abstract Increased use of therapeutic monoclonal antibodies and the relatively high manufacturing costs fuel need for more efficient production methods. Here we introduce a novel, fast, robust, safe isolation platform screening isolating antibody-producing cell lines using nanowell chip an innovative single-cell method. An anti-Her2 antibody producing CHO pool was used as model. The platform; (1) Assures origin clone, (2) Detects individual cells (3) Isolates expands based on their...
Objective: The high cost of orphan drugs limits their access by many patients, especially in low- and middle-income countries. Many are off-patent without alternative generic or biosimilar versions available. Production these at the point-of-care, when feasible, could be a cost-effective alternative. Methods: financial feasibility this approach was estimated setting up small-scale production recombinant human acid alpha-glucosidase (rhGAA). commercial version rhGAA is Myozyme™, Lumizyme™...