A5089831622- Neonatal and Maternal Infections
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Gut microbiota and health
- Tryptophan and brain disorders
- Microbial Natural Products and Biosynthesis
- Otolaryngology and Infectious Diseases
- Trace Elements in Health
- Pneumonia and Respiratory Infections
- Microbial Metabolic Engineering and Bioproduction
- Respiratory viral infections research
- Gene Regulatory Network Analysis
- Mycobacterium research and diagnosis
- Tea Polyphenols and Effects
- Head and Neck Cancer Studies
- Indoor Air Quality and Microbial Exposure
- Sepsis Diagnosis and Treatment
- Mycotoxins in Agriculture and Food
- Dysphagia Assessment and Management
- Infectious Diseases and Tuberculosis
- Fungal Infections and Studies
- Stress Responses and Cortisol
- Infectious Diseases and Mycology
University of Wisconsin–Madison
2015-2023
The Fenton-chemistry-generating properties of copper ions are considered a potent phagolysosome defense against pathogenic microbes, yet our understanding underlying host/microbe dynamics remains unclear. We address this issue in invasive aspergillosis and demonstrate that host fungal responses inextricably connect reactive oxygen intermediate (ROI) mechanisms. Loss the copper-binding transcription factor AceA yields an Aspergillus fumigatus strain displaying increased sensitivity to ROI...
The immune mechanisms that recognize inhaled Aspergillus fumigatus conidia to promote their elimination from the lungs are incompletely understood. FleA is a lectin expressed by has twelve binding sites for fucosylated structures abundant in glycan coats of multiple plant and animal proteins. role unknown: it could bind fucose decomposed matter allow thrive soil, or may be virulence factor binds lung glycoproteins cause pneumonia. Our studies show protein avidly purified mucin...
Fungal spores contain secondary metabolites that can protect them from a multitude of abiotic and biotic stresses. Conidia (asexual spores) the human pathogen Aspergillus fumigatus synthesize several metabolites, including melanin, which has been reported to be important for virulence in this species protective against UV radiation other fungi. Here, we investigate role melanin diverse isolates A. find variability its ability UV-C or impact zebrafish model invasive aspergillosis two clinical...
The study of aflatoxin in
Indoleamine 2,3-dioxygenases (IDOs) degrade l-tryptophan to kynurenines and drive the de novo synthesis of nicotinamide adenine dinucleotide. Unsurprisingly, various invertebrates, vertebrates, even fungi produce IDO. In mammals, IDO1 also serves as a homeostatic regulator, modulating immune response infection via local tryptophan deprivation, active catabolite production, non-enzymatic cell signaling. Whether fungal Idos have pleiotropic functions that impact on host-fungal physiology is...
Abstract Background Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. Method In this study, we report clinical...
Systems biology approaches are extensively used to model and reverse-engineer gene regulatory networks from experimental data. Indoleamine 2,3-dioxygenases (IDOs)—belonging in the heme dioxygenase family—degrade l-tryptophan kynurenines. These enzymes also responsible for de novo synthesis of nicotinamide adenine dinucleotide (NAD+). As such, they expressed by a variety species, including fungi. Interestingly, Aspergillus may degrade not only via IDO but alternative pathways. Deciphering...
Abstract Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. Method: In this study, we report...
Abstract We reviewed the electronic health records of 1,419 inpatients with anterior nares (AN) and oropharynx (OP) MRSA PCR tests. Concordance was 96.5%. In discordant cases, AN negative–OP positive results increased detection probable pneumonia by only 0.3%. A dual testing approach has limited utility in detecting increases resource utilization.
Abstract Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. In this study, we report clinical comparison Xpert...
Abstract Background Anterior nares (AN) MRSA PCR can help identify colonization as a risk factor for pneumonia and be especially useful, given high NPV, in making treatment decisions when lower respiratory tract (LRT) cultures are lacking. Oropharynx (OP) carriage without AN occur suggesting the potential benefit of duel site screening, but doubles resource utilization. We evaluated concordance between OP sites whether addition testing provides clinical benefit. Methods was performed using...
Abstract Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. Method: In this study, we report...
Abstract Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. Method: In this study, we report...
Abstract Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. In this study, we report clinical comparison Xpert...
Abstract Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis newborns. The CDC, therefore recommends GBS screening for all pregnant women at 35–37 weeks gestation administration intrapartum prophylaxis (in those that tested positive) as an effective means controlling disease transmission. Several FDA approved molecular diagnostic tests available rapid accurate detection antepartum women. Method: In this study, we report...