A5027163257- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Rabies epidemiology and control
- Tuberculosis Research and Epidemiology
- Malaria Research and Control
- Animal Disease Management and Epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Phenothiazines and Benzothiazines Synthesis and Activities
- Mesenchymal stem cell research
- Heme Oxygenase-1 and Carbon Monoxide
- Cytokine Signaling Pathways and Interactions
- Ectopic Pregnancy Diagnosis and Management
- Biofuel production and bioconversion
- Drug-Induced Hepatotoxicity and Protection
- Bone Metabolism and Diseases
- interferon and immune responses
- NF-κB Signaling Pathways
- Enzyme Production and Characterization
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Computational Drug Discovery Methods
- Immunodeficiency and Autoimmune Disorders
- Mosquito-borne diseases and control
- Sex work and related issues
University of Belgrade
2016-2024
McMaster University
2024
CReATe Fertility Centre
2023
Ministry of Education, Science and Technological Development
2016
Christie's
2016
Creative Commons
2016
Rutgers, The State University of New Jersey
2004-2014
Rutgers Health
2014
Consumption of undercooked or insufficiently cured meat is a major risk factor for human infection with Toxoplasma gondii. Although horsemeat typically consumed rare undercooked, information on the T. gondii from infected horse to humans scarce. Here, we present results study determine presence in slaughter horses Serbia, and attempt isolate viable parasites. The included all regions Serbia slaughtered at two abattoirs between June 2013 2015. Blood sera were tested specific IgG antibodies by...
Thermotoga neapolitana 1,4-beta-d-glucan glucohydrolase A preferentially hydrolyzes cello-oligomers, such as cellotetraose, releasing single glucose moieties from the reducing end of cello-oligosaccharide chain. Using directed evolution techniques error-prone PCR and mutant library screening, a variant glucan has been isolated that disaccharide, cellobiose, at 31% greater rate than its wild type (WT) predecessor. The library, expressed in Escherichia coli, was screened 85 degrees C for...
Interferon-gamma (IFN-γ) release assays (IGRAs) such as the Quantiferon Gold In-tube test are in vitro that measure IFN-γ from T cells response to M. tuberculosis (Mtb)-specific antigens. Unlike tuberculin skin (TST), IGRA is specific and able distinguish Mtb-infection BCG vaccination. In this study we evaluated concordance between TST efficacy of diagnosing new Mtb infection household contacts (HHC) pulmonary (PTB) cases. A total 357 HHC TB cases Vitória, Brazil were studied. was performed...
To determine the risk of congenital toxoplasmosis (CT) and provide early (pre- or postnatal) identification cases CT in absence systematic screening pregnancy. In presented cross-sectional study, serological criteria were used to date Toxoplasma gondii infection versus conception 80 pregnant women with fetal abnormalities referred as suspected acute infection, 16 after delivery symptomatic neonates. A combination serological, molecular (qPCR), biological (bioassay) methods was for prenatal...
Abstract Although much is understood regarding the role of B7/CD28 family costimulatory molecules in regulating host resistance context several pathogens, analogous information with Mycobacterium tuberculosis lacking. To address requirements B7-mediated costimulation against tuberculosis, mice deficient both B7.1 and B7.2 (B7DKO) were aerosol infected M. Erdman disease progression was monitored. We report herein that B7DKO are initially able to contain bacterial load lung, but exhibit...
Introduction Toxoplasma gondii is an intracellular parasite of importance to human and veterinary health. The structure diversity the genotype population T. varies considerably with respect geography, but three lineages, type I, II III, are distributed globally. Lineage III genotypes least well characterized in terms biology, host immunity virulence. Once a infected T.gondii , innate immune mechanisms engaged reduce burden tissues create pro-inflammatory environment which T H 1 response...
Abstract In this study, the early innate cytokine and chemokine response of murine dendritic cells (DCs) macrophages to Mycobacterium tuberculosis infection was compared. The findings indicate a dissimilar gene expression pattern between two cell types. IL-12 IL-23, important for promoting Th1 Th17 cells, respectively, up-regulated only in DCs. addition, CCL1 CCL17, which are recruitment T regulatory DC-specific, as immunosuppressive IL-10. Macrophages, contrast, exhibited enhanced CCL2...
Toxoplasma gondii is one of the most successful parasites on Earth, infecting a wide array mammals including third global human population. The obligate intracellular protozoon not capable synthesizing cholesterol (Chl), and thus depends uptake host Chl for its own development. To explore genetic regulation previously observed lipid metabolism alterations during acute murine T. infection, we here assessed total fractions in serum selected tissues at pathophysiological molecular level,...
Toxoplasma gondii archetypes II and III are mildly virulent, yet virulence of variant strains is largely unknown. While lineage dominates in humans Europe, present various intermediate hosts. In Serbia, represents 24% the population structure occurs most frequently domestic animals, implying a significant presence human food web. this study, four genetically distinct variants was assessed vivo vitro. vivo, two were shown to be intermediately virulent with cumulative mortalities 69.4%, 38.8%,...
Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure 18 T. gondii strains obtained from slaughter pigs Northern Serbia (mainly Vojvodina). The examined samples originated eight large commercial farms, six backyard and four free-range Mangalica pigs, all found to be positive either viable DNA. Genotyping was attempted both pig tissues mouse brains bio-assays using...
In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of III genotypes varies geographically I are rare, yet present in several regions continent. Data on T. population structure southeastern Europe (SEE) scarce, necessary to appreciate diversity species Europe. To help fill this gap, we genotyped 67 strains from nine intermediate hosts Serbia by MnPCR-RFLP, determined structure, identified using ToxoDB. A neighbor-joining...
Summary Toxoplasma gondii is considered one of the most successful parasites humans and animals. The ingestion viable cysts through consumption undercooked pork recognized as a significant route human infection with T. gondii. aim this study was to investigate presence parasite in tissues free-range pigs from Zasavica Special Nature Reserve. All were Mangulica breed, raised traditional way. serological screening performed using modified agglutination test (MAT). isolation attempted by...
As pork is an important source for Toxoplasma gondii infection, we have analyzed T. genotypes and toxoplasmosis prevalence in pigs Serbia the context of production statistics economics to assess specific risk public health. Genotyping was performed using MnPCR-RFLP; gondii-specific IgG antibodies were detected a modified agglutination test (MAT); statistical data extracted from official records provided by government authorities. The results indicate that, 2006 2021, median number annually...
Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. In vitro testing was performed lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 CQ-resistant (CQR) P. Dd2. vivo evaluated a modified Thompson test using C57BL/6 mice infected with berghei ANKA strain. Nine compounds had lower 50% inhibitory concentration (IC50) than...
Toxoplasma gondii is an obligate intracellular parasite infecting up to one third of the human population. The central event in pathogenesis toxoplasmosis conversion tachyzoites into encysted bradyzoites. A novel approach analyze structure vivo-derived tissue cysts may be increasingly used computational image analysis. objective this study was quantify geometrical complexity T. by morphological, particle, and fractal analysis, as well determine if it impacted strain, cyst age, host type....
Abstract Toxoplasma gondii is an obligate intracellular parasite infecting up to one third of the human population. The central event in pathogenesis toxoplasmosis conversion tachyzoites into encysted bradyzoites. A novel approach analyze structure vivo -derived tissue cysts may be increasingly used computational image analysis. objective this study was quantify geometrical complexity T. by morphological, particle, and fractal analysis, as well determine if how it impacted strain, cyst age,...
62nd International Meat Industry Conference Distribution of Toxoplasma gondii infection on pig farmsWhile T. in domestic animals is generally not a significant clinical issue, it does pose serious economic problem due to substantial losses resulting from spontaneous abortions.It also presents public health concern zoonotic risk.Consuming meat derived asymptomatically infected the most notable risk factor for human infection.The presence has been documented all used consumption, including...
First trimester (FTM) and term human umbilical cord perivascular cells are promising mesenchymal stromal cell candidates to mitigate side effects of oncotherapy, but their safety for cancer patients remains be determined. This study was designed determine if modulate tumor growth when injected systemically in a tumor-bearing mouse model. Immunodeficient mice-bearing palpable subcutaneous SK-MEL-28 melanoma tumors were randomized receive tail vein injection three lines resuspended hank’s...
Abstract Tuberculosis (TB) is a re-emerging health concern infecting nearly one third of the world’s total population and causing 1.5 million human deaths. These bacilli bacteria express multiple favorable adaptations including numerous antibiotic resistant strains. During my summer laboratory experience, I worked on research team within Emerging Re-emerging Disease laboratory. The focus to develop baseline concentration numbers cytokine messengers released by mouse cells after mice are...