A5084905667- Forensic and Genetic Research
- Bacillus and Francisella bacterial research
- HIV Research and Treatment
- HIV-related health complications and treatments
- Molecular Biology Techniques and Applications
- HIV/AIDS drug development and treatment
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Identification and Quantification in Food
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Environmental DNA in Biodiversity Studies
- Animal Disease Management and Epidemiology
- Genetic diversity and population structure
- Respiratory viral infections research
- Genetic and phenotypic traits in livestock
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Plant Pathogenic Bacteria Studies
- Cancer Research and Treatments
- Malaria Research and Control
- Complex Systems and Decision Making
University of Canberra
2011-2020
Australian Federal Police
2020
UNSW Sydney
2020
Children's Hospital at Westmead
2020
Westmead Hospital
2020
The University of Sydney
2020
ACT Government
2018
Australian National University
2013-2016
Monash University
2001-2009
Burnet Institute
2003-2009
Nucleoside reverse transcriptase inhibitors (NRTIs), particularly dideoxynucleoside analogs (ddNs), used in the treatment of HIV, inhibit mitochondrial DNA polymerase gamma vitro. Mitochondrial (mtDNA) depletion is proposed as underlying mechanism many vivo side effects these agents. A reliable and valid laboratory test to detect this not yet available. The objective study was correlate tissue mtDNA quantification HIV-infected patients with exposure nucleoside analogs.60 adults underwent...
Objectives: Nucleoside reverse transcriptase inhibitors (NRTIs), particularly dideoxynucleoside analogs (ddNs), used in the treatment of HIV, inhibit mitochondrial DNA polymerase γ vitro. Mitochondrial (mtDNA) depletion is proposed as underlying mechanism many vivo side effects these agents. A reliable and valid laboratory test to detect this not yet available. The objective study was correlate tissue mtDNA quantification HIV-infected patients with exposure nucleoside analogs. Methods: 60...
Tissue mitochondrial DNA (mtDNA) levels have been proposed as a marker of nucleoside analouge reverse transcriptase inhibitor (NRTI) toxicity. However, clinical studies yielded conflicting data regarding possible associations with mtDNA levels. This study examined in matched samples peripheral blood mononuclear cells (PBMCs) and subcutaneous fat from large Australian cohort to examine treatment, clinical, demographic depletion.mtDNA was quantified by real-time polymerase chain reaction....
Human respiratory syncytial virus (RSV) is a major cause of morbidity and severe lower tract disease in the elderly very young, with some infants developing bronchiolitis, recurrent wheezing, asthma following infection. Previous studies humans animal models have shown that vaccination formalin-inactivated RSV (FI-RSV) leads to prominent airway eosinophilic inflammation challenge; however, roles pulmonary eosinophilia antiviral response pathogenesis are inadequately understood. In vivo mice...
ABSTRACT Human metapneumovirus (HMPV) has been identified as a worldwide agent of serious upper and lower respiratory tract infections in infants young children. HMPV is second only to syncytial virus (RSV) leading cause bronchiolitis, and, like RSV, consists two major genotypes that cocirculate vary among communities year year. Children who have experienced acute infection may develop sequelae wheezing asthma; however, the features contributing this pathology remain unknown. A possible...
Despite anti-TNF therapy advancements for inflammatory diseases such as rheumatoid arthritis, the burden of remains high. An 11-mer TNF peptide, TNF70-80, is known to stimulate selective functional responses compared parent molecule. Here, we show that TNF70-80 binds receptor, activating p38 MAP kinase through receptor-associated factor 2. Using truncated TNFR mutants, identify sequence in TNFRI which enables activation by TNF70-80. Peptides with this sequence, TNFRI206-211 bind and inhibit...
It has been suggested that lipoatrophy associated with exposure to nucleoside analogues is caused by depletion of mitochondrial DNA (mtDNA). The aim the present study was determine whether switching treatment from a thymidine analogue abacavir an increase in mtDNA copy number peripheral blood mononuclear cells (PBMCs). Of 111 patients who were randomized have switched or continue analogues, 94 had PBMCs obtained at baseline and weeks 4, 12, 24, for quantification number. During 24-week...
The use of Salmonella to deliver heterologous antigens from DNA vaccines is a well-accepted extension the success oral in animal models. Attenuated S. typhimurium and typhi strains are safe efficacious, their combines advantages both vaccine approaches, while complementing limitations each technology. An important aspect basic biology Salmonella/DNA platform relative contributions prokaryotic eukaryotic expression production antigen. Gene commonly under control cytomegalovirus (CMV)...
Abstract Hepatitis B virus (HBV) is a pathogen of worldwide health significance, associated with liver disease. A vaccine available, yet HBV prevalence remains concern, particularly in developing countries. Pathology laboratories have primary role the diagnosis and monitoring infection, through hepatitis surface antigen (HBsAg) immunoassay tests. Analysis HBsAg pathology data from 821 Chinese patients applied 10‐fold cross‐validation to establish classification decision trees (CDTs), CDT...
Establishment of national DNA databases in Australia and overseas has increased the number criminal convictions, yet a high volume serious crime cases remain with no suspect profile nor any database matches. In these circumstances prediction biogeographical ancestry (BGA) externally visible characteristics can assist by providing forensic intelligence conjunction with, or place of, eyewitness testimonies. To predict BGA an individual requires: genetic markers selected for their ability to...
Species identification is an important facet of forensic investigation. In this study, human and non-human species (cow, chicken, pig, sheep, cat, dog, rabbit, fox, kangaroo wombat) were assayed on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) to rapidly screen for their origin using high resolution melt (HRM) analysis targeting 16S rRNA gene. Classification HRM difference profiles onboard software resulted in a classification accuracy <20%. Derivative (temperature versus...