A5103906923- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Diabetes Management and Research
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Diabetes and associated disorders
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- Diabetes Treatment and Management
- Cellular transport and secretion
- Toxic Organic Pollutants Impact
- Nicotinic Acetylcholine Receptors Study
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Pancreatitis Pathology and Treatment
- Lipid Membrane Structure and Behavior
- Diet, Metabolism, and Disease
- Air Quality and Health Impacts
- Neuroscience and Neural Engineering
- Cannabis and Cannabinoid Research
- Dam Engineering and Safety
- Geotechnical Engineering and Underground Structures
- Calcium signaling and nucleotide metabolism
- Endoplasmic Reticulum Stress and Disease
University of Gothenburg
2018-2025
Peking University
2006-2020
McGovern Institute for Brain Research
2014-2015
Center for Life Sciences
2011-2015
Institute of Molecular Medicine
2015
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
2015
Chinese Institute for Brain Research
2014
First Automotive Works (China)
2013
Gansu Agricultural University
2009
University of Tsukuba
2001
Glucagon is the body's main hyperglycemic hormone, and its secretion dysregulated in type 2 diabetes mellitus (T2DM). The incretin hormone glucagon-like peptide-1 (GLP-1) released from gut used T2DM therapy. Uniquely, it both stimulates insulin inhibits glucagon thereby lowers plasma glucose levels. In this study, we have investigated action of GLP-1 on release human pancreatic islets. Immunocytochemistry revealed that only <0.5% α-cells possess detectable GLP-1R immunoreactivity. Despite...
By restoring glucose-regulated insulin secretion, glucagon-like peptide-1–based (GLP-1–based) therapies are becoming increasingly important in diabetes care. Normally, the incretins GLP-1 and glucose-dependent insulinotropic polypeptide (GIP) jointly maintain normal blood glucose levels by stimulation of secretion pancreatic β cells. However, reason why only GLP-1–based drugs effective improving after presentation has not been resolved. ATP-sensitive K+ (KATP) channels play a crucial role...
Glucose-induced insulin secretion depends on β-cell electrical activity. Inhibition of ATP-regulated potassium (KATP) channels is a key event in this process. However, KATP channel closure alone not sufficient to induce activity; activation depolarizing membrane current also required. Here we examine the role mechanosensor ion PIEZO1 Yoda1, specific agonist, activates small and thereby triggers activity with resultant stimulation Ca2+-influx secretion. Conversely, antagonist GsMTx4 reduces...
Abstract The generation of pancreatic cell types from renewable sources holds promise for replacement therapies diabetes. Although most effort has focused on generating beta cells, considerable evidence indicates that glucagon secreting alpha cells are critically involved in disease progression and proper glucose control. Here we report the stem cell-derived human (SC-alpha) pluripotent via a transient pre-alpha intermediate. These exhibit transcriptional profile similar to mature although...
Whole-body glucose homeostasis is coordinated through secretion of glucagon and insulin from pancreatic islets. When low, released α-cells to stimulate hepatic production. However, the mechanisms that regulate remain unclear. Here we show in α-cells, interaction between fatty acid oxidation metabolism controls secretion. The glucose-dependent inhibition relies on pyruvate dehydrogenase carnitine palmitoyl transferase 1a activity lowering mitochondrial by increases glucose. This results...
Significance With a combination of HPLC and carbon fiber electrodes, we demonstrate that grafted neural stem cells directly release dopamine in the damaged striatum vivo partially rescue Parkinson’s disease (PD) model. ( i ) Primitive cell–dopamine-like neuron (pNSC–DAn) retained tyrosine hydroxylase expression reduced PD-like asymmetric rotation; ii depolarization-evoked reuptake were significantly rescued vitro (brain slices) vivo, as determined jointly by microdialysis-based...
Key points The timing of synaptic transmission is critical to plasticity in the striatum. However, striatal dopamine (DA) release induced by cholinergic interneurons (ChIs) striatum unclear. In this study, we focused on temporal components DA and replenishment triggered different pathways. We show that stimulation ChIs induces with a total delay 20.6 ms, including 2.8 ms for action potential firing ChIs, 7.0 between acetylcholine terminals terminals, 10.8 downstream release. via ChI pathway...
Abstract Recent work has shown that chemical release during the fundamental cellular process of exocytosis in model cell lines is not all‐or‐none. We tested this theory for vesicular from single pancreatic beta cells. The vesicles these cells insulin, but also serotonin, which detectible with amperometric methods. Traditionally, it assumed Here, we use a multidisciplinary approach involving nanoscale methods to explore nature insulin exocytosis. amperometrically quantified number serotonin...
The pancreatic islet hormones insulin, glucagon and somatostatin play a crucial role in maintaining normoglycaemia. Effective communication between beta, alpha, delta cells is essential for glucose homeostasis disruption of this intra-islet crosstalk observed diabetes. Optogenetics great way to study islets as it eliminates the need drugs that might have off-target effects, while preserving architecture. We generated mouse models optogenetics interrogate physiological humoral response...
By stimulating hepatic glucose production, glucagon (released by islet α-cells) restores normal blood levels when they fall below the range. We used optogenetics in conjunction with electrophysiology, [Ca2+]i imaging and hormone release measurements to explore intrinsic paracrine regulation of secretion. Many α-cells were spontaneously active at 1mM glucose. However, up ∼50% α- cells electrically silent. KATP channel blockade, amino acids somatostatin receptor (SSTR) antagonism restored...
<p dir="ltr"><b>By stimulating hepatic glucose production, glucagon (released by islet α-cells) restores normal blood levels when they fall below the range. We </b><b>used</b><b> optogenetics in conjunction with electrophysiology, [Ca</b><sup><strong>2+</strong></sup><b>]</b><sub><strong>i</strong></sub><b> imaging and hormone release measurements to explore intrinsic paracrine...
<p dir="ltr"><b>By stimulating hepatic glucose production, glucagon (released by islet α-cells) restores normal blood levels when they fall below the range. We </b><b>used</b><b> optogenetics in conjunction with electrophysiology, [Ca</b><sup><strong>2+</strong></sup><b>]</b><sub><strong>i</strong></sub><b> imaging and hormone release measurements to explore intrinsic paracrine...
Action potential (AP) patterns and dopamine (DA) release are known to correlate with rewarding behaviors, but how codes of AP bursts translate into DA in vivo remains elusive. Here, a given pattern was defined by four codes, termed total number, frequency, number bursts, interburst time [N, f, b, i].. The 'burst effect' calculated the ratio (γ) overflow multiple that single burst when fixed. By stimulating medial forebrain bundle using at either physiological (20 Hz) or supraphysiological...
This study demonstrated the unaltered dopamine release in striatum a schizophrenia model <italic>dysbindin</italic>−/− mice.
Abstract Recent work has shown that chemical release during the fundamental cellular process of exocytosis in model cell lines is not all‐or‐none. We tested this theory for vesicular from single pancreatic beta cells. The vesicles these cells insulin, but also serotonin, which detectible with amperometric methods. Traditionally, it assumed Here, we use a multidisciplinary approach involving nanoscale methods to explore nature insulin exocytosis. amperometrically quantified number serotonin...
While glucose-stimulated insulin secretion depends on Ca(2+) influx through voltage-gated channels in the cell membrane of pancreatic β-cell, there is also ample evidence for an important role intracellular stores secretion, particularly relation to drug stimuli. We report here that thiopental, a common anesthetic agent, triggers from intact pancreas and primary cultured rat β-cells. investigated underlying mechanisms by measurements whole K(+) currents, potential, cytoplasmic concentration...