A5086142484- Vector-borne infectious diseases
- Viral Infections and Vectors
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Vector-Borne Animal Diseases
- Pneumonia and Respiratory Infections
- Dermatological diseases and infestations
- Bacterial Infections and Vaccines
- Infectious Diseases and Tuberculosis
- Insect and Pesticide Research
- Infective Endocarditis Diagnosis and Management
- Parasitic Infections and Diagnostics
- Pneumocystis jirovecii pneumonia detection and treatment
- Amoebic Infections and Treatments
- Xenotransplantation and immune response
- Diphtheria, Corynebacterium, and Tetanus
- Infectious Encephalopathies and Encephalitis
- Genetic Neurodegenerative Diseases
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Toxin Mechanisms and Immunotoxins
- Toxoplasma gondii Research Studies
- Ion channel regulation and function
- Animal Disease Management and Epidemiology
National Institute for Public Health and the Environment
1999-2025
Diakonessenhuis hospital
2016-2025
Infectious Diseases and the Research Institute
2000
ABSTRACT The direct repeat region in Mycobacterium tuberculosis complex strains is composed of multiple variant repeats (DVRs), each which a 36-bp (DR) plus nonrepetitive spacer sequence similar size. It has been shown previously that clinical isolates show extensive polymorphism the DR by variable presence DVRs, and this used epidemiology tuberculosis. In an attempt to better understand evolutionary scenario leading polymorphic loci improve strain differentiation spoligotyping, we...
To assess whether the Mycobacterium tuberculosis Beijing genotype is emerging in Vietnam, we analyzed 563 isolates from new cases by spoligotyping and examined association between age, resistance, BCG vaccination status. Three hundred one (54%) patients were infected with strains. The was associated younger age (and hence active transmission) isoniazid streptomycin but not vaccination.
Whooping cough caused by Bordetella pertussis in humans, is re-emerging many countries despite vaccination. Several studies have shown that significant shifts occurred the B. population resulting antigenic divergence between vaccine strains and circulating suggesting pathogen adaptation. In Netherlands, resurgence of associated with rise an altered promoter region for toxin (ptxP3). We used Multi-Locus Sequence Typing (MLST), Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA)...
Bordetella pertussis is the causative agent of human whooping cough (pertussis) and particularly severe in infants. Despite worldwide vaccinations, remains a public health problem. A significant increase incidence has been observed many countries since 1990s. Several reasons for re-emergence this highly contagious disease have suggested. intriguing possibility based on evidence indicating that pathogen adaptation may play role process. In an attempt to gain insight into genomic make-up B....
α-Gal syndrome (AGS) is an emerging tick-borne disease characterised by the development of specific immunoglobulin E (sIgE) antibodies against galactose-α-1,3-galactose (α-gal), a carbohydrate found in most mammalian species, but not humans. AGS occurs individuals with history tick bites. Currently, no data exist on α-gal sIgE seroprevalence Dutch population, including those bites or erythema migrans (EM). Therefore, this study aimed to determine rate assess seroconversion after bites, and...
Abstract In a tuberculosis (TB) program in the Central Penitentiary Hospital of Azerbaijan, we analyzed 65 isolates Mycobacterium by IS6110-based restriction fragment-length polymorphism (RFLP) and spoligotyping. From 11 clusters associated with 33 patients, 31 had an banding pattern characteristic Beijing genotype M. tuberculosis. addition, 15 similar RFLP patterns constituted single group spoligotyping, matching genotype. Multidrug resistance, always involving isoniazid rifampin, was seen...
ABSTRACT Two-tier serology testing is most frequently used for the diagnosis of Lyme borreliosis (LB); however, a positive result no proof active disease. To establish LB, better diagnostics are needed. Tests investigating cellular immune system available, but studies evaluating utility these tests on well-defined patient populations lacking. Therefore, we investigated an enzyme-linked immunosorbent spot (ELISpot) assay to diagnose neuroborreliosis. Peripheral blood mononuclear cells (PBMCs)...
Lyme disease is a common multisystem caused by infection with tick-transmitted spirochete, Borrelia burgdorferi and related species. The monoglycosylated diacylglycerol known as B. glycolipid II (BbGL-II) major target of antibodies in sera from infected individuals. Here, we show that CD1b presents BbGL-II to human T cells the TCR mediates recognition. However, did not detect increased frequency CD1b-BbGL-II binding peripheral blood patients compared controls. Unexpectedly, mapping cell...
Diagnosis of Lyme borreliosis (LB) relies on clinical symptoms and detection Borrelia-specific antibodies. Guidelines recommend a two-tier testing (TTT) strategy for disseminated LB: serological screening with sensitive enzyme immunoassay (EIA) confirmation specific immunoblot. Searching the most approach, this retrospective study evaluated standard (STTT) modified (MTTT) strategies using well-defined population. Cases included patients active neuroborreliosis (LNB; n = 29) or arthritis (LA;...
Commercial cellular tests are used to diagnose Lyme borreliosis (LB), but studies on their clinical validation lacking. This study evaluated the utility of an in-house and a commercial enzyme-linked immunosorbent spot (ELISpot) assay for diagnosis neuroborreliosis (LNB). Prospectively, peripheral blood mononuclear cells (PBMCs) were isolated from patients controls analysed using Borrelia ELISpot LymeSpot assay. B. burgdorferi B31 whole cell lysate mixture outer surface proteins stimulate...
Recent studies have shown elevated levels of the B-cell chemokine (C-X-C motif) ligand 13 (CXCL13) in cerebrospinal fluid (CSF) patients with early Lyme neuroborreliosis (LNB). In this retrospective study, we evaluated diagnostic performance Quantikine CXCL13 enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Inc., MN, USA) and recom Bead (Mikrogen, Neuried, Germany) for detection CSF.
In this retrospective study, the performance of nine serological screening assays for Lyme borreliosis (LB) diagnostics was evaluated using a study population LB cases and controls. Sera derived from 74 well-defined 122 controls were included. The diagnosed with erythema migrans (EM; n = 11), neuroborreliosis (LNB; 35), arthritis (LA; 20), or acrodermatitis chronica atrophicans (ACA; 8). Controls comprised age- gender-matched healthy individuals 48 patients other diseases anticipated high...
The diagnosis of Lyme borreliosis is challenging because the often non-specific symptoms and persisting antibodies after infection. We investigated diagnostic characteristics two enzyme-linked immunosorbent assays (ELISAs) an immunoblot for detection Borrelia-specific serum using different test strategies in individuals with without antibiotic treatment borreliosis. This retrospective study included healthy individuals, patients active neuroborreliosis treated neuroborreliosis. Two ELISAs...
Background: The aim of this study was to evaluate the positive predictive value (PPV) ELISpot in bronchoalveolar lavage (BAL) and pleural fluid for diagnosis active tuberculosis (TB) real-life clinical practice, together with added a cut-off >1.0 ratio between extra-sanguineous systemic interferon-gamma responses samples.Methods: A retrospective, single-centre performed. Patients BAL were included.Results: PPV TB patients (n = 40) 64.9%, which increased 82.6% ESAT-6 panel 71.4% CFP-10 after...
Laboratory diagnosis of Lyme neuroborreliosis (LNB) is challenging, and validated diagnostic algorithms are lacking. Therefore, this retrospective cross-sectional study aimed to compare the performance seven commercial antibody assays for LNB diagnosis. Random forest (RF) modeling was conducted investigate whether using could be improved by including several routine cerebrospinal fluid (CSF) parameters (i.e., leukocyte count, total protein, blood-CSF barrier functionality, intrathecal...
Abstract In a tuberculosis (TB) program in the Central Penitentiary Hospital of Azerbaijan, we analyzed 65 isolates Mycobacterium by IS6110-based restriction fragment-length polymorphism (RFLP) and spoligotyping. From 11 clusters associated with 33 patients, 31 had an banding pattern characteristic Beijing genotype M. tuberculosis. addition, 15 similar RFLP patterns constituted single group spoligotyping, matching genotype. Multidrug resistance, always involving isoniazid rifampin, was seen...
Abstract In a tuberculosis (TB) program in the Central Penitentiary Hospital of Azerbaijan, we analyzed 65 isolates Mycobacterium by IS6110-based restriction fragment-length polymorphism (RFLP) and spoligotyping. From 11 clusters associated with 33 patients, 31 had an banding pattern characteristic Beijing genotype M. tuberculosis. addition, 15 similar RFLP patterns constituted single group spoligotyping, matching genotype. Multidrug resistance, always involving isoniazid rifampin, was seen...
The diagnosis of Lyme neuroborreliosis (LNB) is based on neurological symptoms, cerebrospinal fluid (CSF) pleocytosis, and intrathecally produced Borrelia-specific antibodies. In most cases, the presence antibodies determined by using an enzyme-linked immunosorbent assay (ELISA). edge effect a known phenomenon in ELISAs can negatively influence reproducibility repeatability, as well index calculations sample pairs which are tested same run. For LNB diagnostics, calculation used for relative...