A5100679001- Advanced biosensing and bioanalysis techniques
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Polyoxometalates: Synthesis and Applications
- Bacterial Genetics and Biotechnology
- Metal-Organic Frameworks: Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Heat Transfer and Boiling Studies
- Recommender Systems and Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Millimeter-Wave Propagation and Modeling
- Advanced Fluorescence Microscopy Techniques
- Cellular Automata and Applications
- Gait Recognition and Analysis
- Cell Image Analysis Techniques
- Water Quality Monitoring and Analysis
- Face recognition and analysis
- Advanced Graph Neural Networks
- Embedded Systems Design Techniques
- Biochemical and Structural Characterization
- Plasmonic and Surface Plasmon Research
- Single-cell and spatial transcriptomics
Second Affiliated Hospital of Harbin Medical University
2025
Tianjin University
2022-2025
Harbin Medical University
2025
Nanjing University of Aeronautics and Astronautics
2025
Chinese University of Hong Kong
2024
Peking University
2003-2023
Dalian University of Technology
2023
Hong Kong Baptist University
2022-2023
Center for Life Sciences
2014-2022
Institute of Software
2021
We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves luciferase. Luminescence was induced upon colocalization the reporter ∼44 bp target sequence defined by sgRNAs. used detect Mycobacterium tuberculosis with high specificity and sensitivity. The reprogrammability further leveraged array design that accesses information across entire genome.
Aromatic pollutants in the environments pose significant threat to human health due their persistence and toxicity. Here, we report design comprehensive characterization of a set aromatic biosensors constructed using green fluorescence protein as reporter aromatics-responsive transcriptional regulators, namely, NahR, XylS, HbpR, DmpR, detectors. The genetic connections between detectors were carefully adjusted achieve fold inductions far exceeding those reported previous studies. For each...
Black-odorous water (BOW) in urban areas poses significant risks to safety and human health. Chemical oxygen demand (COD) is a critical parameter for the control monitoring of BOW. However, traditional methods COD determination are expensive, time-consuming, involve use hazardous chemicals. In this study, reduced graphene oxide (rGO) transitional metal particles (Cu, Ni) were used as working electrode materials facile on-site Three composite (rGO/Cu, rGO/Ni, rGO/Cu/Ni) synthesized by...
Abnormal glycolytic metabolism plays a significant role in pulmonary vascular remodeling idiopathic arterial hypertension (IPAH), yet the specific mechanisms remain unclear. The primary objective of this study is to investigate key regulatory glycolysis IPAH. Bulk and single-cell sequencing data obtained from IPAH patient tissue samples were downloaded GEO database. scMetabolism AUCcell analyses carried out quantify metabolic activity identify main cell types regulating glycolysis,...
The development of high-performance and high-density packaging electronic devices has led to the concentration heat generation in smaller areas, resulting formation "hotspots", i.e., regions high flux. Localized temperatures can adversely affect performance components or cause thermal stress. Therefore, there is an urgent need develop thin, cooling that achieve effective dissipation confined spaces. Micro/nano-fabrication technologies offer solutions address these challenges. Heat pipes, as...
Synthetic Biology aims to create predictable biological circuits and fully operational systems. Although there are methods more stable oscillators, such as repressilators, independently controlling the oscillation of reporter genes in terms their amplitude period is only on theoretical level. Here, we introduce a new oscillator circuit that can be controlled by two inducers Escherichia coli. Some control components, including σECF11 NahR, were added circuit. By systematically tuning...
A defining goal of synthetic biology is to develop biomaterials with superior performance and versatility. Here we introduce a purely genetically encoded self-assembling biopolymer based on the SpyTag–SpyCatcher chemistry. We show application this polymer for highly efficient uranyl binding extraction from aqueous solutions, by embedding two functional modules—the superuranyl protein monomeric streptavidin—to via genetic fusion. further provide modeling strategy predicting polymer's physical...
Polyoxometalates (POMs) are three-dimensional materials with unique, exceptional physical and chemical characteristics. The performance of POM-derived is anticipated to be enhanced by the combination POM metal–organic frameworks (MOFs) due high surface areas MOF materials. In this study, three kinds T-POMs@ZIF-67 (T-PMo@ZIF-67, T-SiW@ZIF-67, T-PW@ZIF-67) were prepared doping a cobalt-based (ZIF-67) POMs (phosphomolybdic acid, silicotungstic phosphotungstic acid). results show that power...
Convolutional neural networks have been verified to be exceptionally powerful on extracting semantic features, which contribute a great progress in computer vision. However, focusing too much the superiority, researchers seem pay less attention exploring CNNs' potential other aspects, e.g. ability discriminate difference. In this work we try dig into discriminative power of CNNs and introduce deep network with inception module (DDN-IM) for person re-identification. Without individual feature...
With the increasing prominence of pollution issues in offshore waters, marine ranching plays a crucial role restoring ecosystems and ensuring sustainable use fishery resources. This paper provides comprehensive bibliometric analysis global research. The study indicates that policy is primary driver industry's development. It also reveals low level interconnectivity between China other major developing countries, as well cooperation governmental research institutions area ranching. In light...
In this study, we report on how to design efficient catalysts for glucose oxidation via the transitional metal doping of nanohybrids polyoxometalates (POMs) and metal-organic frameworks (MOFs). ZIF-67, a cobalt-based MOF, as well phosphomolybdic acid (PMo), were used precursors fabrication pyrolyzed PMo@ZIF-67 (T-PMo@ZIF-67). A different amount Ni2+ was doped into produce NixCoy@T-PMo@ZIF-67. Among them, Ni2Co2@T-PMo@ZIF-67 had best performance. The power density fuel cell that an anode...
DNA origami can be applied as a "ruler" for nanoscale calibration or super-resolution fluorescence microscopy with an ideal structure defining fluorophore arrangement, allowing the distance between fluorophores to precisely controlled at nanometer scale. also used nanotag arbitrary programmable shapes topological identification. In this study, we formed hexagonal embedded three different fluorescent dyes on surface. The each block was ~120 nm, which is below diffraction limit of light, its...
In recent years, the International Genetically Engineered Machine (iGEM) competition has experienced rapid global development. 2017 alone, number of iGEM teams registered around globe reached an unprecedented 313, with 98 from China having enrolled in and obtained outstanding results. contrast to many college students' innovation projects scientific research training programs China, iGEM's organization mode is focused on student-centered learning. Moreover, it achieved a rich educational...
This paper introduces MedTiny, a streamlined component-based modeling language, evolved from Mediator. Existing languages struggle to provide convenience in expressing recurrent component configurations, which are common modern large-scale parallel computation models distributed environments. Furthermore, Mediator frequently conducts repetitive flag manipulations synchronize between entities. To address these issues, we propose syntactic enhancements, including array with static foreach...
Various genetic switches have been developed to let engineered cells perform designed functions. However, a sustained input is often needed maintain the on/off state, which energy-consuming and sensitive perturbation. Therefore, we set of transcriptional for cell states control that were constructed by inversion effect site-specific recombinases on terminators. Such switch could respond pulse signal new state itself until next input. With bottom-up design principle, first characterized...
Abstract Genetic regulation is achieved by monitoring multiple levels of gene expression, from transcription to protein interaction. Unlike common temporary transcriptional methods such as the use inducible promoters, integrases permanently edit DNA sequences. Integrases, however, require especially strict when implemented in synthetic genetic systems because irreversible result. Here we propose improve site-specific integrase-based system dynamically hiding one attB/P sites that are...
Nowadays, since most information is transmitted by public medias, the security of very important, especially confidential information. The system has its particular processing method. use cryptographic machine one effective solutions. hidden trouble mainly comes from kernel program and key chip. And in China, being menaced, for chip involving devices come abroad. Developing chip-set a hard chief task China. A research on system-level architecture single-chip data presented. involves...