A5048120892- Ultrasound and Hyperthermia Applications
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ultrasound and Cavitation Phenomena
- Photoacoustic and Ultrasonic Imaging
- Nanoparticle-Based Drug Delivery
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
- Fluid Dynamics and Mixing
- Characterization and Applications of Magnetic Nanoparticles
- Lipid Membrane Structure and Behavior
- Liquid Crystal Research Advancements
- Biosensors and Analytical Detection
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Minerals Flotation and Separation Techniques
- Electrohydrodynamics and Fluid Dynamics
- Quantum Information and Cryptography
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Pancreatic and Hepatic Oncology Research
- Quantum Mechanics and Applications
University of Leeds
2016-2025
St James's University Hospital
2018-2024
Wellcome Trust
2020-2023
Mansoura University
2021
University of Hull
2008-2015
NIHR Imperial Biomedical Research Centre
2010
Imperial College London
2010
Because of their size (1-10 μm), microbubble-based drug delivery agents suffer from confinement to the vasculature, limiting tumor penetration and potentially reducing efficacy. Nanobubbles (NBs) have emerged as promising candidates for ultrasound-triggered because small size, allowing complexes take advantage enhanced permeability retention effect. In this study, we describe a simple method production nested-nanobubbles (Nested-NBs) by encapsulation NBs (∼100 nm) within drug-loaded...
An extremely versatile microfluidic device is demonstrated in which multi-step (bio)chemical procedures can be performed continuous flow. The system operates by generating several co-laminar flow streams, contain reagents for specific (bio)reactions across a rectangular reaction chamber. Functionalized magnetic microparticles are employed as mobile solid-supports and pulled from one side of the chamber to other use an external field. As particles traverse reagent binding washing steps on...
Micron sized, lipid stabilized bubbles of gas are interest as contrast agents for ultra-sound (US) imaging and increasingly delivery vehicles targeted, triggered, therapeutic delivery. Microfluidics provides a reproducible means microbubble production surface functionalisation. In this study, microbubbles generated on chip using flow-focussing microfluidic devices that combine streams liquid through nozzle few microns wide then subjecting the two phases to downstream pressure drop. While...
We present the first on-chip atomisation-like production of phase-change contrast agents at high concentrations towards high-resolution imaging for diagnostic ultrasound.
Lipid coated liquid crystal droplets have been trapped in a novel trap structure for the on-chip detection of model antimicrobial peptide – Smp43, an α-helical from Scorpion Venom.
Most cancer patients receive chemotherapy at some stage of their treatment which makes improving the efficacy cytotoxic drugs an ongoing and important goal. Despite large numbers potent anti-cancer agents being developed, a major obstacle to clinical translation remains inability deliver therapeutic doses tumor without causing intolerable side effects. To address this problem, there has been intense interest in nanoformulations targeted delivery improve outcomes. The aim work was demonstrate...
Drug penetration into solid tumours remains a major challenge in the effective treatment of cancer. Microbubble (MB) mediated sonoporation offers potential solution to this by enhancing uptake drugs cells. Additionally, using an ultrasound (US) trigger, drug delivery can be localised tumour, thus reducing off-site toxicity associated with systemic delivery. The majority vitro studies involving observation MB-enhanced efficacy have been conducted on 2D monolayer cell cultures, which are known...
Lipid-shelled nanobubbles (NBs) are emerging as potential dual diagnostic and therapeutic agents. Similar to their micron-scale counterparts, microbubbles (1-10 μm), they can act ultrasound contrast agents well locally enhance uptake. Recently, it has been shown that the reduced size of NBs (<1 μm) promotes increased uptake accumulation in tumor interstitial space, which performance. However, accurate characterization NB concentration is challenging may limit translation into clinical use....
For a series of phospholipid coated calamitic nematic liquid crystal droplets (5CB, 6CB, 7CB, E7 and MLC7023) diameter ∼18 μm, the addition chiral dopant leaves sign surface anchoring unchanged. Herein we report that for these an analyte induced transition from Frank-Pryce structure (with planar anchoring) to nested-cup perpendicular is accompanied by changes in intensity reflected light. We propose this system as both general scheme understanding director fields with ideal candidate be...
Nanoscale colloidal self-assembly is an exciting approach to yield superstructures with properties distinct from those of individual nanoparticles. However, the bottom-up 3D nanoparticle typically requires extensive chemical functionalization, harsh conditions, and a long preparation time, which are undesirable for biomedical applications. Here, we report directional freezing porous silica nanoparticles (PSiNPs) as simple versatile technique create anisotropic hierarchical porosity afforded...
Microbubbles are potential diagnostic and therapeutic agents. In vivo stability is important as the bubbles required to survive multiple passages through heart lungs allow targeting delivery. Here we have systematically varied key parameters affecting microbubble lifetime significantly increase in stability. Whilst shell core composition found an role improving stability, show that inclusion of small quantities C6F14 bolus improves lifetime. Our results indicate inserts into lipid shell,...
Abstract Label‐free live single‐cell Raman spectroscopy was used to obtain a chemical fingerprint of colorectal cancer cells including the classification SW480 and SW620 cell line model system, derived from primary secondary tumour same patient. High‐quality spectra were acquired hundreds cells, showing high reproducibility between experiments. Principal component analysis with linear discriminant yielded best classification, an accuracy 98.7 ± 0.3% (standard error) when compared...
We introduce a novel and extremely versatile microfluidic platform in which tedious multi-step biochemical processes can be performed continuous flow within fraction of the time required for conventional methods.
We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar streams reagents and washing solutions via an external magnet, for rapid detection inflammatory biomarker, C-reactive protein (CRP). The two-step sandwich immunoassay was accomplished less than 60 s, vast improvement on 80–300 min time frame required enzyme-linked immunosorbent assays (ELISA) 50 necessary off-chip particle-based assays....
Microbubbles (MBs) are increasingly being proposed as delivery vehicles for targeted therapeutics, well contrast agents ultrasound imaging. MBs formed with a lipid shell promising candidates due to their biocompatibility and the opportunity surface functionalization, both specific targeting of tissues means tune mechanical response localized induced destruction in vivo. Herein, we acquired force-deformation data on coated using tip-less microcantilevers an atomic force microscope. Model were...
We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets. At high enough lipid concentration we show that the de-wetting of octanol from these droplets occurs spontaneously (off-chip) without need to use shear aid process. The resultant mixture and GUVs can be separated by buoyancy octanol. A simpler device pump system employed and, because higher flow-rates much rate formation (∼1500 s-1 compared up ∼75...
Microbubbles (MBs) have a multitude of applications including as contrast agents in ultrasound imaging and therapeutic drug delivery vehicles, with further scope for combining their diagnostic properties (known theranostics). MBs used clinically are commonly made by mechanical agitation or sonication methods, which offer little control over population size dispersity. Furthermore, yet to be therapeutically research is needed develop these theranostic agents. In this paper, we present our MB...
Despite improvements in the understanding of disease biology, pancreatic ductal adenocarcinoma (PDAC) remains most malignant cancer pancreas. PDAC constitutes ∼95% all cancers, and it is highly resistant to therapeutics. The increased tissue rigidity, which stems from rich fibrotic stroma tumor microenvironment, central development, physiology, resistance drug perfusion. Pancreatic stellate cells (PSCs) are responsible for overproduction extracellular matrix stroma, this exacerbated by...
Developing tumour models that recapitulate the biophysical barriers lead to drug resistance in cancer is critical assessing new drugs and delivery mechanisms.