Thiol groups are important anti-oxidants and essential molecules protecting organism against the harmful effects of reactive oxygen species (ROS). The aim our study is to evaluate thiol-disulphide homeostasis with a novel recent automated method in patients localized prostate cancer (PC) before six months after radical prostatectomy (RP). 18 PC 17 healthy control subjects were enrolled into study. Blood samples collected from controls RP. Thiol-disulphide was determined using recently...

Objectives: Recently, the need for early diagnosis of modifiable risk factors involved in etiology stroke has been highlighted literature. Nesfatin-1 is a peptide expressed central nervous system and peripheral tissues used as biomarker recent years. This study aimed to determine association ischemic with internal carotid artery stenosis according nesfatin-1 level whether it could be biomarker. Methods: A total 118 patients were included study. Three groups defined: acute symptomatic...

Abstract Some experimental and clinical studies have been conducted for the usage of chemotherapeutic drugs encapsulated into nanoparticles (NPs). However, no study has so far on co-encapsulation doxorubicin (Dox) epoxomicin (Epo) NPs as biocompatible drug delivery carriers. Therefore, we investigated if and/or enhance their anticancer efficiency prevent resistance toxicity to normal cells. We synthesized Dox Epo loaded poly (lactic-co-glycolic acid) (PLGA) using a multiple emulsion solvent...

Insulin treatment can lead to good glycemic control and result in improvement of lipid parameters type 2 diabetic patients. This study was designed evaluate the effect insulin analog initiation therapy on low-density lipoprotein (LDL)/ high-density (HDL) sub-fractions HDL associated enzymes patients during early phase. Twenty four with glycosylated hemoglobin (HbA1c) levels above 10% despite ongoing combination sulphonylurea metformin were selected. Former regimen continued for first day...

Breast cancer is a worldwide commonly found malignancy in women and effective treatment regarded as huge clinical challenge even the presence of several options. Extensive literature available demonstrating polyphenols phytopharmaceutical anticancer agents. Among polyphenols, quercetin curcumin have been reported to strong potential against breast cancer. However, so far, no comprehensive study has performed demonstrate anticarcinogenic effects curcumin, quercetin, their combinations with...

The importance of sapienic acid (6c-16:1), a monounsaturated fatty the n-10 family formed from palmitic by delta-6 desaturase, and its metabolism to 8c-18:1 sebaleic (5c,8c-18:2) has been recently assessed in cancer. Data are lacking on association between signaling cascades exposure comparing cell lines same cancer type. We used 50 μM supplementation, non-toxic concentration, cultivate MCF-7 2 triple-negative breast cells (TNBC), MDA-MB-231 BT-20. followed up for three hours regarding...

Abstract Antioxidants may prevent apoptosis of cancer cells via inhibiting reactive oxygen species (ROS). However, to date no study has been carried out elucidate the effects strong antioxidant N ‐acetylcysteine (NAC) on Bleomycin induced in human testicular (NTERA‐2, NT2) cells. For this reason, we studied and NAC alone combination apoptotic signaling pathways NT2 cell line. We determined cytotoxic effect bleomycin measured markers such as Caspase‐3, ‐8, ‐9 activities Bcl‐2, Bax, Cyt‐c,...

Vitamin C (Vit C) is an important physiological antioxidant with growing applications in cancer. Somatostatin (SST) a natural peptide growth inhibitory effect several mammary cancer models. The combined effects of SST and Vit supplementation have never been studied breast cells so far. We used MCF-7 MDA-MB231 incubated for 24h, the absence presence C, at their EC50 concentrations, to evaluate membrane fatty acid-profiles together follow-up EGFR MAPK signaling pathways. two cell lines gave...

This study aimed to investigate LDL subfraction distribution as well serum cholesteryl ester transfer protein (CETP), lecithin-cholesterol acyltransferase (LCAT), and paraoxonase (PON1) activity in streptozotocin-induced diabetic guinea pigs. Materials/Methods . Guinea pigs were given a single intraperitoneal (ip) injection of streptozotocin (STZ) animals having fasting blood glucose levels greater than 200 mg/dl, considered diabetic. Protein LCAT CETP determined via ELISA. Paraoxonase was...

Ovarian torsion is agynecologic emergency that affects females of all ages. Early diagnosis important to preserve ovarian function. The false positive rate for sonographic 50%; therefore, a new real-time approach required improve diagnostic accuracy. We investigated diffuse reflectance spectroscopy diagnosing torsion. Spectroscopic measurements were performed in vivo prior to, during and after detorsion. After bilateral oophorectomy, hemoxygenase myeloperoxidase enzyme activity tissue was...

Background Multiple sclerosis (MS) is a neuroinflammatory disease and inflammation oxidative stress play important roles in its pathology. Thiol/disulphide homeostasis (TDH) special biomarker that has been found to be affected several disorders including MS. There no study demonstrating the effects of attack status relapsing-remitting multiple (RRMS) patients on TDH levels. Our aim was determine levels three different periods RRMS healthy individuals. Methods The carried out 29 with without...

Copeptin, the C-terminal fragment of antidiuretic hormone, is a new biomarker that has been found to be elevated in several disorders and could related with prognosis. This study aimed compare plasma copeptin levels patients restless legs syndrome (RLS) healthy individuals investigate whether were associated severity disease.41 primary RLS, who followed Bakirkoy Psychiatry Neurology Research Training Hospital 41 age- sex-matched included into study. RLS divided subgroups as mild-moderate,...

Abstract Background The triple-negative breast cancer (TNBC) subtype, characterized by loss of HER2, estrogen, and progesterone receptors, displays aggressive phenotype poor prognosis compared to other BC subtypes. Since the TNBC cells are devoid endocrine therapy is an ineffective option for patients, necessitating canonical chemotherapy strategies treat TNBC. It crucial use alternative natural agents support in Objectives To clarify molecular mechanism tumorigenic effects gambogic acid...

Apoptosis detection methods cause the cell culture medium to be physically or chemically affected.In addition, these have disadvantages such as cost and losses.The aim of this study is develop a method assess apoptosis in real-time using back-reflection spectroscopy (BRS) breast cancer cells.BRS experiment set up consists spectrometer, tungsten -halogen light source fiber optic probe.In order changes associated with apoptosis, intensity reflected was measured -optic probe from MCF7 samples...

Near-infrared spectroscopy (NIRS) is widely used to assess flap perfusions by measuring tissue oxygen saturation (StO2). However, the StO2 level for onset of perfusion failure still a controversial issue.This study proposes new threshold detecting as early possible increase salvage rates.Twenty patients undergoing surgery were included in this - 13 flaps implemented cover defects that occurred due trauma and 7 hide imperfections after cancer treatment. Thirteen lower extremity, six mandible,...

The free flap is a unit of tissue and transferred from one site to another with the aim improving form function while maintaining its own blood supply. A perfusion sufficient oxygenation important for survival flaps. gold standard method monitoring has yet be established. Many failing flaps can salvaged early identification prompt intervention. Therefore clinicians underscores importance reliable postoperative monitoring. this study develop spectroscopic system running non-invasive in real...

In this work, we have developed a new portable optical device to assess tissue microcirculation. Our system is based on the idea that can calculate oxygen saturation by analyzing interaction with light at two different wavelengths. The involves measuring quantitative value using information obtained from light-tissue sensors designed functionally connect patient. For purpose; LEDs which enable emission in near-infrared wavelengths and photodiode capable of intensity reflected were used...