Single-stranded telomeric DNA tends to form a four-base-paired planar structure termed G-quadruplex. This was easily formed in vitro the presence of monovalent cations. However, existence this native human telomeres is unclear. Here we address important question through distinctive properties 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) upon binding various structures. Although fluorescence BMVC increases significantly DNA, has high sensitivity and preference quadruplex...

Control of colon cell fate in adenocarcinomas is disrupted, part, due to aberrant Wnt/β-catenin signaling. The nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) has been implicated the development cancers. In adenomatous polyposis coli multiple intestinal neoplasia (APC Min ) mouse cancer model, PPARγ expression colonic mucosa markedly altered. addition, protein levels are elevated, possibly through sequestration by activated β-catenin lines. Induction pathway LiCl also...

Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of ammonia and glutamate to yield glutamine, ADP, inorganic phosphate in presence divalent cations. Bacterial GS is an enzyme 12 identical subunits, arranged two rings 6, with active site between each pair subunits a ring. In earlier work, we have reported locations within funnel-shaped substrates ATP cations, but for (or ammonium) has remained elusive. Here report discovery by X-ray crystallography binding on monovalent Tl+...

Abstract We have investigated a novel compound, 3,6‐bis[2‐(1‐methylpyridinium)vinyl]carbazole diiodide ( BMVC ), for inhibiting telomerase activity and distinguishing human lung H1299 oral Ca9‐22 cancer cells from IMR90 skin Detroit‐551 normal fibroblast cells. The telomeric repeat amplification protocol (TRAP) assay shows that the concentration of inhibits 50% IC 50 ) is ca. 0.05 μ M . On other hand, cell‐viability indicates cytotoxicity was less than 15% to cells, almost negligible MRC‐5...

Electrocardiogram (ECG) is the primary basis for diagnosis of cardiovascular diseases. However, amount ECG data patients makes manual interpretation time-consuming and onerous. Therefore, intelligent recognition technology an important means to decrease shortage medical resources. This study proposes a novel classification method arrhythmia that uses very first time three-heartbeat multi-lead (THML) in which each fragment contains three complete heartbeat processes multiple leads. The THML...

Carbazole derivatives that stabilized G-quadruplex DNA structure formed by human telomeric sequence have been designed and synthesized. Among them, 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) showed an increase in melting temperature 13 degrees C has a potent inhibitory effect on telomerase activity. Treatment of H1299 cancer cells with 0.5 mumol/L BMVC did not cause acute toxicity affect replication; however, the BMVC-treated ceased to divide after lag period. Hallmarks...

ABSTRACT Objectives Subglottic stenosis (SGS) is a challenging complication post‐airway interventions. Effective preventive strategies lack sufficient evidence. This study investigated the effect of inhalation therapy with dexamethasone/ciprofloxacin on acute SGS in rabbit model. Methods Twenty New Zealand White rabbits underwent subglottic injury via endoscopy and received twice daily starting day. Group 1 ( n = 4) saline for 5 days was sacrificed day 5; 2 3 same survived one week, 15; 4 10...

Premature ventricular contraction (PVC) is one of the most common arrhythmias which can cause palpitation, cardiac arrest, and other symptoms affecting work rest activities a patient. However, patients hardly decipher their own feelings to determine severity disease thus, requiring professional medical diagnosis. This study proposes novel method based on image processing convolutional neural network (CNN) extract electrocardiography (ECG) curves from scanned ECG images derived clinical...

Atrial fibrillation (AF) is the most common cardiovascular disease (CVD), and existing algorithms are usually designed for diagnosis (i.e., feature classification) or prediction of AF. Artificial intelligence (AI) integrate AF electrocardiogram (ECG) predict possibility that will occur in future. In this paper, we utilized MIT-BIH Database (AFDB), which composed data from normal people patients with onset characteristics, AFPDB database PAF Prediction Challenge Database), consists Paroxysmal...

Abstract The aim of this study was to assess associations between fat pad areas at various anatomic levels and the sites lateral wall collapse disease severity in adult patients with obstructive sleep apnea (OSA). Forty-one OSA who prospectively underwent drug-induced computed tomography were included. Areas parapharyngeal pads degrees three representative (nasopharynx, oropharynx, subglosso-supraglottis), apnea-hypopnea index (AHI) measured. In subglosso-supraglottic region, area 17 (41%)...

This study aims to explore the factors that contribute epiglottic collapse (EC) in patients with obstructive sleep apnea (OSA).This enrolled 35 (34 males; median age, 39 years; apnea-hypopnea index (AHI), 55.4 events/h; body mass (BMI), 26.9 kg/m2). EC (epiglottis attaching onto posterior pharyngeal wall) was diagnosed by drug-induced computed tomography (DI-SCT). Three dimensions were assessed for comparison between and non-EC (NEC) groups included anatomical measurement: length angle,...

This work demonstrates the feasibility of gallium nitride (GaN)-based light-emitting diodes (LEDs) with a metal-via-hole structure embedded in reflector on backside sapphire substrate. Luminescence intensity for surface-emitting LEDs is enhanced by mirroring efficaciously downward light emitted from InGaN/GaN multiquantum wells (MQWs) owing to deep ladder-shaped inclined A ladder shape was also processed wet-etching method at high temperature pattern substrate deeply. The electroplating used...

Oral cavity squamous cell carcinoma (OSCC) is a leading cause of cancer-related deaths worldwide and associated with poor prognosis mortality. Discovery proteins that can improve OSCC treatment needed. Using comparative proteome profiling primary cells derived from adjacent noncancerous epithelium, endoplasmic reticulum aminopeptidases 2 (ERAP2) has been identified as an OSCC-associated protein. Compared the normal tissues, ERAP2 levels were determined to be significantly elevated in tissues...

In electrocardiograms (ECGs), multiple forms of encryption and preservation formats create difficulties for data sharing retrospective disease analysis. Additionally, photography storage using mobile devices are convenient, but the images acquired contain different noise interferences. To address this problem, a suite novel methodologies was proposed converting paper-recorded ECGs into digital data. Firstly, study ingeniously removed gridlines by utilizing Hue Saturation Value (HSV) spatial...

Objective A surgical response to upper airway (UA) surgery for obstructive sleep apnea (OSA) depends on adequate correction of collapsible sites in the UA. This pilot study aimed examine UA directed by drug‐induced computed tomography (DI‐SCT) OSA. Study Design Prospective case series. Setting Tertiary referral center. Subjects and Methods recruited 29 OSA patients (median age, 41 years; median body mass index, 26.9 kg/m 2 ) who underwent single‐stage DI‐SCT‐directed between October 2012...

Abstract Induced circular dichroism has been used to study the binding of thionin molecule twelve guanine-rich quadruplexes. Substituting base in loops and varying length tails could substantial change induced spectra, which allow us distinguish structural isomers determine sites Our results show that external stacking on end surface G-quartet associated with electrostatic interaction or is major mode these G-quadruplexes.

Objective/Hypothesis To assess the ability of ultra‐short echo time (UTE)‐MRI to detect subglottic stenosis (SGS) and evaluate response balloon dilation. correlate measurements from UTE‐MRI with endotracheal‐tube (ETT)‐sizing investigate whether SGS causes change in airway dynamics. Study Design Animal research study. Methods Eight adult New‐Zealand white rabbits were used as they approximate neonatal airway‐size. The airways measured using ETT‐sizing 3D at baseline, 2 weeks...

This study proposes a design of multi-channel EEG acquisition device based on Bluetooth microcontroller for continuous collection signals. Composed an analog front-end circuit and microcontroller, it can be connected to universal electrode strip through hardware interface. It record high-quality signals from eight channels. The system realizes the collection, analog-to-digital conversion, data transmission set sampling rate 250Hz 1KHz software programs. meets requirements portability test...