A5017836121- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Sirtuins and Resveratrol in Medicine
- Nerve injury and regeneration
- Adipokines, Inflammation, and Metabolic Diseases
- Stochastic Gradient Optimization Techniques
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Cardiovascular Disease and Adiposity
- Sparse and Compressive Sensing Techniques
- Domain Adaptation and Few-Shot Learning
- Pancreatic function and diabetes
- PARP inhibition in cancer therapy
- Caveolin-1 and cellular processes
- Platelet Disorders and Treatments
- Mesenchymal stem cell research
- Markov Chains and Monte Carlo Methods
- Wound Healing and Treatments
- Advancements in Transdermal Drug Delivery
- Single-cell and spatial transcriptomics
- Immune responses and vaccinations
University of South Carolina
2015-2024
Veterans Health Administration
2021-2022
University of California, Davis
2008-2013
Northwestern University
2013
Baylor College of Medicine
2009-2010
Houston Methodist
2009-2010
Temple University
2009
University of Alabama at Birmingham
2009
University of Houston
2009
Background— Monocyte activation and migration into the arterial wall are key events in atherogenesis associated with hypercholesterolemia. CD11c/CD18, a β 2 integrin expressed on human monocytes subset of mouse monocytes, has been shown to play distinct role monocyte adhesion endothelial cells, but regulation CD11c hypercholesterolemia its unknown. Methods Results— Mice genetically deficient were generated crossbred apolipoprotein E (apoE) −/− mice generate /apoE mice. Using flow cytometry,...
Objective— Atherosclerosis is associated with monocyte adhesion to the arterial wall that involves integrin activation and emigration across inflamed endothelium. Involvement of β 2 -integrin CD11c/CD18 in atherogenesis was recently shown dyslipidemic mice, which motivates our study its inflammatory function during hypertriglyceridemia humans. Methods Results— Flow cytometry blood from healthy subjects fed a standardized high-fat meal revealed at 3.5 hours postprandial, CD11c surface...
Breast cancer is a leading cause of death for women, with mortality resulting from metastasis. Metastases are often detected once tumour cells affect the function solid organs, high disease burden limiting effective treatment. Here we report method early detection metastasis using an implanted scaffold to recruit and capture metastatic in vivo, which achieves cell densities reduces within organs 10-fold. Recruitment associated infiltration immune cells, include Gr1hiCD11b+ cells. We identify...
Abstract Mac-1-dependent crawling is a new step in the leukocyte recruitment cascade that follows LFA-1-dependent adhesion and precedes emigration. Neutrophil via LFA-1 has been shown to induce cytoskeletal reorganization through Vav1-dependent signaling, current study investigates role of Vav1 process vivo with particular attention events immediately downstream adhesion. Intravital spinning-disk-confocal microscopy was used investigate intravascular relation endothelial junctions wild-type...
Islet transplantation is a promising treatment for human type 1 diabetes mellitus. Transplantation requires systemic immunosuppression, which has numerous deleterious side effects. antigen-specific regulatory T cells (Tregs) have been shown to protect islet grafts from autoimmune destruction in the nonobese diabetic (NOD) model when co-localized kidney capsule. An extra-hepatic transplant site was established by transplanting islet-loaded microporous poly (lactide-co-glycolide) (PLG)...
ABSTRACT Objective : Atherosclerosis is a focal disease that develops at sites of low and oscillatory shear stress in arteries. This study aimed to understand how endothelial cells sense gradient fluid transduce signals regulate membrane expression cell adhesion molecules monocyte recruitment. Methods Human aortic were stimulated with TNF‐α simultaneously exposed linear increased from 0 16 dyne/cm 2 . Cell molecule activation NFκ B quantified by immunofluorescence microscopy resolution the...
Resveratrol (RSV) and nicotinamide (NAM) have garnered considerable attention due to their anti-inflammatory anti-aging properties. NAM is a transient inhibitor of class III histone deacetylase SIRTs (silent mating type information regulation 2 homologs) SIRT1 an poly-ADP-ribose polymerase-1 (PARP1). The debate on the relationship between RSV has precluded use as therapeutic drug. Recent work demonstrated that facilitates tyrosyl-tRNA synthetase (TyrRS)-dependent activation PARP1. Moreover,...
Monocyte recruitment to inflamed arterial endothelium initiates plaque formation and drives progression of atherosclerosis. Three distinct monocyte subsets are detected in circulation (CD14 ++ CD16 − , CD14 + ), each may play roles during atherogenesis myocardial infarction. We studied a range subjects that included otherwise healthy patients with elevated serum triglyceride levels presenting acute Our objective was correlate an individual’s risk the activation state subset as function...
Endothelial cells (EC) derived from embryonic stem (ESC) require additional functional characterization before they are used as a cell therapy in order to enhance their potential for engraftment and proliferation. We explore several physiologically relevant functions of ESC-derived EC (ESC-EC), such its capacity produce nitric oxide (NO), regulate permeability, activate express surface molecules the recruitment leukocytes response inflammatory stimuli, migrate grow new blood vessels, lay...
As biomaterial therapies emerge to address adipose tissue dysfunction that underlies metabolic disease, the immune response these systems must be established. a potential therapy, we are investigating resveratrol delivery from porous poly(lactide-co-glycolide) scaffolds designed integrate with tissue. Resveratrol was selected for its ability protect mice and primates high fat diet broad anti-inflammatory properties. Herein, report fabrication of loading stable active up one year. In vitro...
Abstract Lipid overload of the adipose tissue, which can be caused by overnutrition, underlies metabolic disease. We hypothesized that increasing energy demand tissue is a promising strategy to combat excessive lipid accumulation. Resveratrol, natural polyphenol, activates catabolism in fat tissue; however, its clinical success hindered poor bioavailability. Here, we implanted resveratrol releasing poly(lactide‐co‐glycolide) scaffolds into epididymal overcome bioavailability with goal...
Ectopic lipid accumulation, the deposition of lipids in lean tissue, is linked to type 2 diabetes through an association with insulin resistance. It occurs when adipose tissue fails meet storage needs and there spillover into tissues not equipped store them. contributes organ dysfunction because can interfere signaling other pathways. Clinical studies indicate that decreasing ectopic diet exercise effective treating diabetes; however, its prevalence continues rise. We propose strategies...
Stochastic Gradient Descent (SGD) is being used routinely for optimizing non-convex functions. Yet, the standard convergence theory SGD in smooth setting gives a slow sublinear to stationary point. In this work, we provide several theorems showing global minimum problems satisfying some extra structural assumptions. particular, focus on two large classes of structured functions: (i) Quasar (Strongly) Convex functions (a generalization convex functions) and (ii) Polyak-Lojasiewicz condition...
The oil-in-water emulsion/solvent extraction method is used to fabricate many FDA approved, polymer particle formulations for drug delivery. However, these do not benefit from surface functionalization that can be achieved by tuning chemistry. Poly(vinyl alcohol) (PVA) the emulsifier approved and remains associated with after fabrication. We hypothesized hydroxyl groups in PVA could conjugated biomolecules using isothiocyanate chemistry modifications would endow additional functionality....
We develop EigenVI, an eigenvalue-based approach for black-box variational inference (BBVI). EigenVI constructs its approximations from orthogonal function expansions. For distributions over $\mathbb{R}^D$, the lowest order term in these expansions provides a Gaussian approximation, while higher-order terms provide systematic way to model non-Gaussianity. These are flexible enough complex (multimodal, asymmetric), but they simple that one can calculate their low-order moments and draw...