A5042290852- Sexual function and dysfunction studies
- Hormonal and reproductive studies
- Nitric Oxide and Endothelin Effects
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Glycosylation and Glycoproteins Research
- Eicosanoids and Hypertension Pharmacology
- Atherosclerosis and Cardiovascular Diseases
- Inflammasome and immune disorders
- Urinary Bladder and Prostate Research
- Neuroendocrine regulation and behavior
- Pain Mechanisms and Treatments
- Heart Rate Variability and Autonomic Control
- Renin-Angiotensin System Studies
- Galectins and Cancer Biology
- Cardiovascular Disease and Adiposity
- Carbohydrate Chemistry and Synthesis
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Ion Channels and Receptors
- Ion Transport and Channel Regulation
- Hypothalamic control of reproductive hormones
- Adipose Tissue and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Universidade de Ribeirão Preto
2014-2024
Universidade de São Paulo
2015-2024
Clinics Hospital of Ribeirão Preto
2013-2019
Augusta University
2008-2018
Weatherford College
2018
Silva
2018
University of Glasgow
2016
San Fernando General Hospital
2013-2015
Michigan State University
2008
Universidade Federal de Minas Gerais
2008
Vascular aging is the term that describes structural and functional disturbances of vasculature with advancing aging. The molecular mechanisms aging-associated endothelial dysfunction are complex, but reduced nitric oxide (NO) bioavailability altered vascular expression activity NO synthase (NOS) enzymes have been implicated as major players. Impaired relaxation in has attributed to NOS (eNOS)-derived NO, while increased inducible (iNOS) seems account for nitrosative stress disrupted...
Although a correlation between polymorphisms of NOD-like receptor family-pyrin domain containing 3 (NLRP3) and predisposition to type 1 diabetes (T1D) has been identified, the potential function activation NLRP3 inflammasome in T1D have not clarified. The present study shows that non-obese diabetic mice exhibited increased NLRP3, pro-IL-1β gene expression pancreatic lymph nodes (PLNs). Similar increases apoptosis associated speck like protein (ASC) were induced by multiple low doses...
Disturbances in the regulation of cytosolic calcium (Ca 2+ ) concentration play a key role vascular dysfunction associated with arterial hypertension. Stromal interaction molecules (STIMs) and Orai proteins represent novel mechanism to control store-operated Ca entry. Although STIMs act as sensors for intracellular stores, is putative pore-forming component release–activated channels at plasma membrane. We hypothesized that augmented activation /Orai-1, through enhanced activity STIM-1,...
Androgen receptors are widely distributed in several tissues, including vascular endothelial and smooth muscle cells. Through classic cytosolic androgen or membrane receptors, testosterone induces genomic non-genomic effects, respectively. Testosterone interferes with the function by increasing production of pro-inflammatory cytokines arterial thickness. Experimental evidence indicates that sex steroid hormones, such as modulate synthesis bioavailability NO and, consequently, function, which...
Research Topic: Effects of Androgens on Immunity to Self and Foreign Background: Both supraphysiological subphysiological testosterone levels are associated with increased cardiovascular risk. Testosterone consumption at doses has been linked blood pressure, left ventricular hypertrophy, vascular dysfunction, inflammatory markers. Activation the NLRP3 inflammasome contributes production proinflammatory cytokines, leading dysfunction. We hypothesized that testosterone, via generation...
Hyperglycemia, which increases O-linked beta-N-acetylglucosamine (O-GlcNAc) proteins, leads to changes in vascular reactivity. Because dysfunction is a key feature of arterial hypertension, we hypothesized that vessels from deoxycorticosterone acetate and salt (DOCA-salt) rats exhibit increased O-GlcNAc associated with reactivity constrictor stimuli. Aortas DOCA exhibited contraction phenylephrine (E(max) [mN]=17.6+/-4 versus 10.7+/-2 control; n=6) decreased relaxation acetylcholine...
Glycosylation with β-N-acetylglucosamine (O-GlcNAcylation) is one of the most complex post-translational modifications. The cycling O-GlcNAc controlled by two enzymes: UDP-NAc transferase (OGT) and O-GlcNAcase (OGA). We recently reported that endothelin-1 (ET-1) augments vascular levels O-GlcNAcylated proteins. Here we tested hypothesis O-GlcNAcylation contributes to effects ET-1 via activation RhoA/Rho-kinase pathway.Incubation smooth muscle cells (VSMCs) (0.1 μM) produces a time-dependent...
This study tested the hypothesis that adenosine, in murine corpora cavernosa, produces direct relaxation of smooth muscle cells and inhibition contractile responses mediated by sympathetic nerve stimulation. Penes were excised from anesthetized male C57BL/6 mice, dissected, cavernosal strips mounted to record isometric force. Adenosine, 2-chloroadenosine (stable analog adenosine), 2-phenylaminoadenosine (CV1808) (A2<sub>A</sub>/A2<sub>B</sub> agonist) produced concentration-dependent...
Extracellular signal-regulated kinase (ERK)1/2 has been reported to play a role in vascular dysfunction associated with mineralocorticoid hypertension. We hypothesized that, compared female rats, an upregulation of ERK1/2 signaling the vasculature male rats contributes augmented contractile responses Uninephrectomized and Sprague-Dawley received desoxycorticosterone acetate (DOCA) pellets (200 mg per animal) saline drink for 3 weeks. Control uninephrectomized tap water drink. Blood pressure,...
Recent evidence suggests that angiotensin II (Ang II) upregulates phosphodiesterase (PDE) 1A expression. We hypothesized Ang augmented PDE1 activation, decreasing the bioavailability of cyclic guanosine 3' 5'-monophosphate (cGMP), and contributing to increased vascular contractility. Male Sprague-Dawley rats received mini-osmotic pumps with (60 ng·min(-1)) or saline for 14 days. Phenylephrine (PE)-induced contractions were in aorta (E(max)168% ± 8% vs 136% 4%) small mesenteric arteries (SMA;...
Oxidative stress plays an important role in diabetes mellitus (DM)–associated vascular injury. DM is risk factor for erectile dysfunction. Functional and structural changes internal pudendal arteries (IPA) can lead to We hypothesized that downregulation of nuclear E2–related 2 (Nrf2), consequent increased nicotinamide adenine dinucleotide phosphate oxidase 1 (NOX1)-derived reactive oxygen species (ROS), impairs IPA function DM. smooth muscle cells from C57BL/6 (control) NOX1 knockout mice...
Metabolic and vascular dysfunction are common features of obesity. Aryl hydrocarbon receptor (AhR) regulates lipid metabolism homeostasis, but whether AhR activated in obesity or have a protective and/or harmful effects on function unknown. Our study addresses activation contributes to obesity-associated the mechanisms involved these effects.Male KO (Ahr-/- ) WT mice were fed either control HF (high-fat) diet for 10 weeks. inflammatory parameters measured serum adipose tissue. Vascular...
O-GlcNAcylation augments vascular contractile responses, and O-GlcNAc-proteins are increased in the vasculature of deoxycorticosterone-acetate salt rats. Because endothelin 1 (ET-1) plays a major role dysfunction associated with salt-sensitive forms hypertension, we hypothesized that ET-1–induced changes responses mediated by O-GlcNAc modification proteins. Incubation rat aortas ET-1 (0.1 μmol/L) produced time-dependent increase levels decreased expression O -GlcNAc transferase...