A5048412151- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Spinal Dysraphism and Malformations
- Axon Guidance and Neuronal Signaling
- RNA Research and Splicing
- Multiple Sclerosis Research Studies
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Microtubule and mitosis dynamics
- Neurogenetic and Muscular Disorders Research
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Cytokine Signaling Pathways and Interactions
- Hereditary Neurological Disorders
- Retinoids in leukemia and cellular processes
- Adipose Tissue and Metabolism
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Olfactory and Sensory Function Studies
- Cholesterol and Lipid Metabolism
- Spinal Cord Injury Research
- Circular RNAs in diseases
Sorbonne Université
2015-2025
Institut du Cerveau
2015-2025
Allen Institute for Brain Science
2025
Pitié-Salpêtrière Hospital
2009-2024
Assistance Publique – Hôpitaux de Paris
2009-2024
Inserm
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Université Paris Cité
2014-2015
Centre de Gestion Scientifique
2015
Sorbonne Paris Cité
2015
Abstract Identifying a source of cells with the capacity to generate oligodendrocytes in adult CNS would help development strategies promote remyelination. In present study, we examined ability precursor mouse subventricular zone (SVZ) differentiate into remyelinating oligodendrocytes. After lysolecithin‐induced demyelination corpus callosum, progenitors rostral SVZ (SVZa) and migratory pathway (RMS), expressing embryonic polysialylated form neural cell adhesion molecule (PSA‐NCAM),...
The destiny of the mitotically active cells subventricular zone (SVZ) in adult rodents is to migrate olfactory bulb, where they contribute replacement granular and periglomerular neurons. However, these neural progenitors also can be mobilized periventricular white matter triggered differentiate into astrocytes oligodendrocytes response lysolecithin-induced demyelination. To mimic environmental conditions multiple sclerosis, we assessed proliferation, migration, differentiation potential SVZ...
In multiple sclerosis (MS), oligodendrocyte and myelin destruction lead to demyelination with subsequent axonal loss. Experimental in rodents has highlighted the activation of subventricular zone (SVZ) involvement progenitor cells expressing polysialylated form neural cell adhesion molecule (PSA-NCAM) repair process. this article, we studied distribution early PSA-NCAM + progenitors SVZ MS lesions human postmortem brains. Compared controls, showed a 2- 3-fold increase density proliferation,...
The basic helix-loop-helix transcription factor Olig2 is a key determinant for the specification of neural precursor cells into oligodendrocyte progenitor cells. However, functional role in migration and differentiation remains elusive both during developmental myelination under demyelinating conditions adult central nervous system. To decipher functions, we generated transgenic mice (TetOlig2:Sox10rtTA/+) overexpressing Sox10+ oligodendroglial doxycycline inducible manner. We show that...
Oligodendrocytes are the myelin-forming cells of CNS. They differentiate from oligodendrocyte precursor (OPCs) that produced progenitors throughout life but more actively during neonatal period and in response to demyelinating insults. An accurate regulation oligodendrogenesis is required generate oligodendrocytes these developmental or repair processes. We hypothesized this implicates transcription factors, which expressed by OPCs and/or their progenitors. Ascl1/Mash1 a proneural factor...
The mechanisms regulating differentiation of oligodendrocyte (OLG) progenitor cells (OPCs) into mature OLGs are key to understanding myelination and remyelination. Signaling via the retinoid X receptor γ (RXR-γ) has been shown be a positive regulator OPC differentiation. However, nuclear (NR) binding partner RXR-γ not established. In this study we show that binds several NRs in OPCs OLGs, one which is vitamin D (VDR). Using pharmacological knockdown approaches RXR-VDR signaling induces VDR...
In demyelinating diseases such as Multiple Sclerosis (MS), demyelination of neuronal fibers impairs impulse conduction and causes axon degeneration. While activity stimulates oligodendrocyte production myelination in normal conditions, it remains unclear whether the demyelinated axons restores their loss-of-function a harmful environment. To investigate this question, we established model to induce moderate optogenetic stimulation corpus callosum at level motor cortex which cortical circuit...
The gcm gene of Drosophila melanogaster encodes a transcription factor that is an important component in cell fate specification within the nervous system. In absence functional gene, progenitor cells differentiate into neurons, whereas when ectopically expressed produce excess glial at expense neuronal differentiation. Recent searches databases have uncovered high sequence similarity between and anonymous human placental cDNA clone (Altschuller et al., 1996; this communication). Here we...
Abstract We have investigated the expression of highly polysialylated neural cell adhesion molecule in mouse spinal cord during postnatal myelination and adult after chemically induced demyelination. By double immunohistochemistry, using a monoclonal antibody (anti‐Men B) which specifically recognizes polysialic acid (PSA) units on (N‐CAM), an anti‐myelin basic protein, caudorostral gradient PSA‐NCAM was observed at day 1 (P1), inversely related to myelination. At P7, labelling decreased...
Microarray analysis of oligodendrocyte lineage cells purified by fluorescence-activated cell sorting (FACS) from 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP)–enhanced green fluorescent protein (EGFP) transgenic mice revealed Sox17 (SRY-box containing gene 17) expression to be coordinately regulated with that four myelin genes during postnatal development. In CNP–EGFP-positive (CNP–EGFP + ) cells, mRNA and levels transiently increased between days 2 15, white matter O4...
Neural stem cells (NSCs) persist in defined brain niches, including the subventricular zone (SVZ), throughout adulthood and generate new neurons destined to support specific neurological functions. Whether diseases such as multiple sclerosis (MS) are associated with changes adult NSCs whether this might contribute development and/or persistence of deficits remains poorly investigated. We examined SVZ function mice which we targeted an MS-like pathology forebrain. In these mice, refer herein...
Significance Liver X receptors (LXRs) α and β are the two major of oxysterols, oxygenated derivatives cholesterol. They control homeostasis cholesterol, an important lipid constituent myelin sheaths. In central nervous system, these insulating structures generated by oligodendrocytes stabilized proteins. Here, we provide evidence a new role LXRs in physiology cerebellum. Mice invalidated for both exhibit alteration motor coordination spatial learning linked with myelination deficits. We...
A variety of markers are invaluable for identifying and purifying stem/progenitor cells. Here we report the generation a murine reporter line driven by Pw1 that reveals cycling quiescent progenitor/stem cells in all adult tissues thus far examined, including intestine, blood, testis, central nervous system, bone, skeletal muscle, skin. Neurospheres generated from PW1-reporter mouse show near 100% reporter-gene expression following single passage. Furthermore, epidermal stem can be purified...
Autism spectrum disorders (ASD) are neuropsychiatric diseases characterized by impaired social interaction, communication deficits, and repetitive stereotyped behaviors. ASD etiology is unknown, both genetic environmental causes have been proposed. Different brain structures believed to play a role in ASD-related behaviors, including medial prefrontal cortex (mPFC), Hippocampus, Piriform (Pir), Basolateral Amygdala (BLA) Cerebellum. Compelling evidence suggests link between white matter...
Abstract Impaired cognitive functioning is a core feature of schizophrenia, and hypothesized to be due myelination as well interneuron defects during adolescent prefrontal cortex (PFC) development. Here we report that in the apomorphine-susceptible (APO-SUS) rat model, which has schizophrenia-like features, defect occurred specifically parvalbumin interneurons. The adult rats displayed medial PFC (mPFC)-dependent inflexibility, reduced number mature oligodendrocytes myelinated inhibitory...
The synthesis of platelet-derived growth factor-α receptor (PDGF-αR) is commonly attributed to oligodendrocyte progenitors during late embryonic and postnatal development. However, we recently demonstrated that mature neurons could also synthesize PDGF-αR, emphasizing a larger role for this than previously described. In the present study, analyze pattern PDGF-αR expression development mouse CNS, used in situ hybridization immunohistochemistry on brain spinal cord tissue sections. We found...
Experimental studies provided overwhelming proof that transplants of myelin-forming cells achieve efficient remyelination in the CNS. Among cellular candidates, Schwann can be used for autologous transplantation to ensure robust lesions and deliver therapeutic factors In present study, macaque expressing green fluorescent protein (GFP) were infected with human immunodeficiency virus-derived vectors overexpressing brain-derived neurotrophic factor (BDNF) or Neurotrophin 3 (NT-3), two...