A5018048866- Mosquito-borne diseases and control
- Malaria Research and Control
- Toxoplasma gondii Research Studies
- Digital Imaging for Blood Diseases
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Trypanosoma species research and implications
- Smart Agriculture and AI
- Vector-borne infectious diseases
- Parasitic Infections and Diagnostics
- Image Processing Techniques and Applications
- Public Health and Social Inequalities
- Viral Infections and Vectors
- Burn Injury Management and Outcomes
- Injury Epidemiology and Prevention
- Libraries, Manuscripts, and Books
- Agriculture and Farm Safety
- Chromosomal and Genetic Variations
- Clinical Laboratory Practices and Quality Control
- Virology and Viral Diseases
- Rabies epidemiology and control
- Global Maternal and Child Health
- Diverse Scientific Research Studies
- Educational Methods and Psychological Studies
- Research on Leishmaniasis Studies
Instituto Nacional de Salud
2008-2024
National Institute of Health
2021
Division of Parasitic Diseases and Malaria
2020
Center for Global Health
2020
Centers for Disease Control and Prevention
2020
Instituto Nacional de Salud
2014-2018
District University of Bogotá
2011
Instituto Multidisciplinario de Biología Celular
1982-1986
With the aim of determining prevalence asymptomatic Plasmodium spp. infection by thick smear and PCR its association with demographic epidemiological characteristics in village Nuevo Tay, Tierralta, Córdoba, Colombia, a cross-sectional population study was carried out, using random probabilistic sampling. Venous blood samples were taken from 212 people on day 0 for PCR. Clinical follow-up smears out days 14 28. The 17.9% (38/212; 95% CI: 12.5-23.3%) Plasmodiumspp. 14.6% (31/212; 9.6-19.6%)....
Abstract Background The World Health Organization (WHO) provides protocols for the diagnosis of malaria. One them is related to staining process blood samples guarantee correct parasite visualization. Ensuring quality procedure on thick smears (TBS) a difficult task, especially in rural centres, where there are factors that can affect smear (e.g. types reagents employed, place sample preparation, among others). This work presents an analysis image-based approach evaluate coloration TBS used...
Artemether–lumefantrine (AL) is the first-line treatment for uncomplicated Plasmodium falciparum infection in Colombia. To assess AL efficacy malaria Quibdo, Choco, Colombia, we conducted a 28-day therapeutic study (TES) following WHO guidelines. From July 2018 to February 2019, febrile patients aged 5–65 years with microscopy-confirmed P. mono-infection and asexual parasite density of 250–100,000 parasites/µL were enrolled treated supervised 3-day course AL. The primary endpoint was...
Evidence on the comorbidity between soil-transmitted helminth infections and malaria is scarce divergent. This study explored interactions uncomplicated falciparum in an endemic area of Colombia. A paired case-control matched by sex, age location Tierralta, Cordoba, was done January September 2010. The incident cases were 68 patients with 178 asymptomatic controls. questionnaire used to gather information sociodemographic variables. Additionally physical examinations carried out, stool...
The decrease in the efficacy of antimalarial drugs world and Colombia hampers its control.The vivo therapeutic amodiaquine+sulfadoxine-pyrimethamine combination was evaluated treatment uncomplicated Plasmodium falciparum malaria chloroquine for P. vivax malaria.From May to November 2006, studies treatments were undertaken Tierralta, Córdoba, northeastern Colombia. Standard protocols followed as recommended by World Health Organization/Panamerican Organization, with some modifications....
This study seeks to address the critical knowledge gap surrounding acute phase of Chagas disease in Colombia, with a specific focus on cases reported 2019. The is pivotal period for intervention, yet it remains poorly understood, particularly regions where oral transmission presumed be significant factor. By analyzing these recent cases, our research aims provide deeper understanding dynamics during its Colombia essential not only improving management and treatment strategies but also...
Resistance to anti-malarial drugs is associated with polymorphisms in target genes and surveillance for these molecular markers important detect the emergence of mutations drug resistance signal recovering sensitivity anti-malarials previously used.The presence Plasmodium falciparum chloroquine sulfadoxine-pyrimethamine was evaluated by Sanger sequencing, 85 P. day enrollment samples from a therapeutic efficacy study artemether-lumefantrine conducted 2018-2019 Quibdo, Colombia. Samples were...
Introducción. La implementación y desarrollo de actividades del sistema gestión la calidad diagnóstico malaria, permiten el adecuado funcionamiento red nacional diagnóstico, necesario para fortalecer acciones prevención control este evento, importante en salud pública.Objetivo. Caracterizar Red Diagnóstico Malaria Colombia, entre 2006 2010. Materiales métodos. Se hizo un estudio retrospectivo mediante revisión los informes anuales Malaria, enviados por laboratorios pública analizó cobertura...
Determinar la seroprevalencia de enfermedad Chagas en población general procedente tres departamentos Amazonía colombiana: Vaupés, Amazonas y Guaviare analizar variables riesgo para enfermedad. Métodos. Para determinar seropositividad se analizaron 3429 muestras suero obtenidas mediante previo consentimiento informado durante los años 2009 2010 a través un muestreo probabilístico, conglomerados, estratificado trietápico cada departamento, con probabilidades finales desiguales. Fueron...
El Instituto Nacional de Salud Colombia, con el fin conocer la prevalencia toxoplasmosis en trabajadores mataderos, realizó determinación anticuerpos contra Toxoplasma gondii población seleccionada. objetivo fue determinar seroprevalencia toxoplasmosis, mediante técnica ELFA (Enzyme Linked Fluorescent Assay), plantas beneficio animal cinco ciudades del país durante año 2008, . Se llevó a cabo un estudio corte transversal 400 las que trabajan área limpia, quienes fueron seleccionados forma...
En este artículo se describe el caso clínico de una mujer colombiana del sur país con diagnóstico infección intrauterina por Toxoplasma gondii y los virus chikungunya Zika. La acudió al control prenatal en segundo trimestre su embarazo e informó que durante primer había presentado síntomas indicativos Mediante reacción cadena la polimerasa (PCR) líquido amniótico, demostró así como las imágenes diagnósticas observaron malformaciones sistema nervioso central feto. A 29 semanas gestación dio...
The implementation and development of activities the quality assurance system malaria diagnosis, allows adequate operation national diagnostic network, needed to strengthen prevention control actions this important public health problem.To characterize diagnosis network in Colombia between 2006 2010.A retrospective study was made by reviewing annual reports that were sent Public Health Laboratories (PHL) 2010. included analysis coverage population at risk an evaluation training people...
Introducción. Dadas las dificultades del diagnóstico microscópico de la malaria o paludismo en áreas rurales, pruebas rápido constituyen una buena alternativa, por lo que es importante conocer su desempeño.Objetivo. Evaluar el desempeño utilizadas cinco departamentos para al usando reacción cadena polimerasa (PCR) como estándar referencia.Materiales y métodos. Se usaron prueba gota gruesa y, además, se impregnó papel filtro con sangre molecular (PCR), individuos sintomáticos.Resultados....
Medir el grado de concordancia entre las técnicas inmunofluorescencia indirecta y ELISA, empleadas para la detección anticuerpos IgG anti-Toxoplasma gondii en humanos. Se llevó a cabo un estudio descriptivo comparativo dos diagnósticas anti-T. gondii, 243 sueros humanos del banco muestras Laboratorio Parasitología-Red Nacional Laboratorios Instituto Salud, recolectados durante los años 2000 2006. El porcentaje reactividad fue 54% (131 sueros) por técnica IFI 56,3% (137 ELISA; además, 46%...
To increase the accessibility of malaria diagnosis, Instituto Nacional de Salud Colombia undertook a field trial to evaluate sensitivity and specificity two rapid diagnostic tests.The sensitivity, concordance was compared for tests malaria, NOW ICT Malaria Pf/Pv OptiMAL.A descriptive study performed with 214 patients in southwestern coastal city Tumaco, Colombia, each whom presented at least one symptoms classical triad. Two strategies were applied patient recruitment-one by passive search...
Introduction: There are no reports describing polymorphisms in target genes of anti-Toxoplasma drugs South American isolates.Objective: This study sought to perform cloning and sequencing the dihydrofolate reductase (dhfr) dihydropteroate-synthase (dhps) reference Rh strain two Colombian isolates Toxoplasma gondii.Materials methods: Two were obtained from cerebrospinal fluid HIV-infected patients with cerebral toxoplasmosis. A DNA extraction technique PCR assay for dhfr dhps standardized,...
This study reports a challenging diagnosis of Plasmodium ovale malaria in Colombian citizen returning from Cameroon. Initial microscopy screenings conducted at two private hospitals yielded conflicting results, with the first showing negative smears and second diagnosing P. vivax. Subsequent examinations government laboratories identified ovale, although routine species-specific PCR strategy was negative. confirmation finally obtained when wallikeri primers were used. Although is not...
El aislamiento de T. gondii a partir líquido cefalorraquídeo permite el diagnóstico confirmatorio toxoplasmosis cerebral, considerada la segunda infección oportunista más importante en pacientes VIH positivos. Aislar Toxoplasma muestras dos positivos con cerebral. Se realizó inoculación intraperitoneal del sedimento LCR cerebral ratones ICR, se llevaron cabo 5 pases sucesivos ciegos hasta observación taquizoítos exudado peritoneal mediante examen directo y coloración extendidos Romanowsky...
Battling malaria's morbidity and mortality rates demands innovative methods related to malaria diagnosis. Thick blood smears (TBS) are the gold standard for diagnosing malaria, but their coloration quality is dependent on supplies adherence protocols. Machine learning has been proposed automate diagnosis, impact of smear parasite detection not yet fully explored.
Introducción. Como parte del plan de eliminación la malaria en Colombia, se propuso desarrollar actividades enmarcadas línea trabajo: “Mejorar el acceso y calidad diagnóstico malaria”.Objetivo. Comparar metodología recomendada por Organización Panamericana Salus con utilizada Colombia para malaria.Materiales métodos. Se recolectaron muestras prepararon 88 láminas malaria, bajo diferentes tratamientos según los parámetros evaluados. Después lectura microscópica duplicado, hicieron respectivos...
Optimizar las condiciones de amplificación del gen B1 (35 copias en el genoma) para la detección ADN T.gondii casos probables toxoplasmosis cerebral. Materiales y métodos: Se realizó extracción a partir exudado peritoneal ratones inoculados con cepa RH T. gondii obteniendo 17 mL una concentración inicial 1x107 parásitos/mL. optimizaron PCR B1. Resultados: obtuvo un fragmento 132 pb obtenido diluciones seriadas desde 1x106 1x10-1 parásitos por mL, estableciéndose límite 1 taquizoíto gondii.
Abstract Background: The World Health Organization (WHO) provides protocols for the diagnosis of malaria. One them is related to staining process blood samples guarantee correct parasite visualization. Ensuring quality procedure on thick smears (TBS) a dicult task, especially in rural centers, where there are factors that can ect smear (e.g. types reagents employed, place sample preparation, among others). This work presents an analysis image-based approach evaluate coloration TBS used...