A5017497107- Malaria Research and Control
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Venomous Animal Envenomation and Studies
- Vector-borne infectious diseases
- Computational Drug Discovery Methods
- HIV/AIDS drug development and treatment
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Bacteriophages and microbial interactions
- Digital Radiography and Breast Imaging
- Studies on Chitinases and Chitosanases
- Parasitic Diseases Research and Treatment
- Enzyme Structure and Function
- Travel-related health issues
- Genetic factors in colorectal cancer
- Calpain Protease Function and Regulation
- Genetic Mapping and Diversity in Plants and Animals
- Head and Neck Cancer Studies
- Leptospirosis research and findings
- Aquaculture disease management and microbiota
- Journalism and Media Studies
- Cancer survivorship and care
Instituto Nacional de Salud
2010-2024
Division of Parasitic Diseases and Malaria
2020
Center for Global Health
2020
Centers for Disease Control and Prevention
2020
Fundación Pública Galega de Medicina Xenómica
2018
Universidad Nacional de Colombia
2016
Universidad El Bosque
2016
CIBBIM-Nanomedicine
2006
University of North Carolina at Chapel Hill
1997
Calera (United States)
1995
With the aim of determining prevalence asymptomatic Plasmodium spp. infection by thick smear and PCR its association with demographic epidemiological characteristics in village Nuevo Tay, Tierralta, Córdoba, Colombia, a cross-sectional population study was carried out, using random probabilistic sampling. Venous blood samples were taken from 212 people on day 0 for PCR. Clinical follow-up smears out days 14 28. The 17.9% (38/212; 95% CI: 12.5-23.3%) Plasmodiumspp. 14.6% (31/212; 9.6-19.6%)....
Background Acute undifferentiated febrile illness is a common challenge for clinicians, especially in tropical and subtropical countries. Incorrect or delayed diagnosis of patients may result medical complications preventable deaths. Common causes acute Colombia include leptospirosis, rickettsioses, dengue fever, malaria, chikungunya, Zika virus infection. In this study, we described the postmortem reported as suspected cases leptospirosis through national surveillance Colombia, 2016–2019....
The hypoxanthine phosphoribosyltransferase (HPRT) from Trypanosoma cruzi is a potential target for enzyme structure-based inhibitor design, based on previous studies which indicate that these parasites lack the metabolic enzymes required de novo synthesis of purine nucleotides. By using bacterial complement selection system, 59 analogs were assayed their interaction with HPRTs T. and Homo sapiens. Eight compounds identified assay to have an affinity trypanosomal enzyme. Inhibition constants...
Asymptomatic infection by Plasmodium spp. could help explain the dynamics of malaria transmission in low-transmission regions. We therefore compared prevalence asymptomatic two Colombian locations, Tierralta and Tumaco, with different patterns, ecological epidemiological characteristics potentially associated factors. Two cross-sectional studies were carried out between September 2006 November 2007. Infection was determined using polymerase chain reaction (PCR) microscopy. Prevalence at day...
Artemether–lumefantrine (AL) is the first-line treatment for uncomplicated Plasmodium falciparum infection in Colombia. To assess AL efficacy malaria Quibdo, Choco, Colombia, we conducted a 28-day therapeutic study (TES) following WHO guidelines. From July 2018 to February 2019, febrile patients aged 5–65 years with microscopy-confirmed P. mono-infection and asexual parasite density of 250–100,000 parasites/µL were enrolled treated supervised 3-day course AL. The primary endpoint was...
The therapeutic efficacy of sulfadoxine-pyrimethamine (SP) in treating uncomplicated Plasmodium falciparum malaria is unevenly distributed Colombia. Andes mountain range separates regions the west where endemic from those east and constitutes a barrier against gene flow dispersal parasite populations. distribution dhfr dhps genotypes 146 P. samples eastern Amazon Orinoco basins Northwest Southwest Pacific Colombia was consistent with documented levels SP. diversity four dhfr- dhps-linked...
Evidence on the comorbidity between soil-transmitted helminth infections and malaria is scarce divergent. This study explored interactions uncomplicated falciparum in an endemic area of Colombia. A paired case-control matched by sex, age location Tierralta, Cordoba, was done January September 2010. The incident cases were 68 patients with 178 asymptomatic controls. questionnaire used to gather information sociodemographic variables. Additionally physical examinations carried out, stool...
The production of recombinant proteins is essential for the characterization and functional study from Plasmodium falciparum. However, P. falciparum are among most challenging to express, when expression achieved, usually fold incorrectly lead formation inclusion bodies. To obtain purify four use them as antigens produce polyclonal antibodies. efficiency solubility were evaluated expressed in two genetically modified strains Escherichia coli favor heterologous (BL21-CodonPlus (DE3)-RIL...
The decrease in the efficacy of antimalarial drugs world and Colombia hampers its control.The vivo therapeutic amodiaquine+sulfadoxine-pyrimethamine combination was evaluated treatment uncomplicated Plasmodium falciparum malaria chloroquine for P. vivax malaria.From May to November 2006, studies treatments were undertaken Tierralta, Córdoba, northeastern Colombia. Standard protocols followed as recommended by World Health Organization/Panamerican Organization, with some modifications....
We evaluated the Plasmodium vivax polymorphism by studying Pvmsp-3 gene's polymorphic region PCR-RFLP in 55 samples from patients living Tierralta, Colombia. Three different sizes of gene were found, type A (1,900 bp), B (1,500 bp) and C (1,100 bp); most (96.4 %). The exhibited high polymorphism. Seven restriction patterns found when using Alu I, nine with Hha I; 12 alleles obtained these combined. findings suggest that this could be used Colombia as a molecular epidemiologic marker for...
This study seeks to address the critical knowledge gap surrounding acute phase of Chagas disease in Colombia, with a specific focus on cases reported 2019. The is pivotal period for intervention, yet it remains poorly understood, particularly regions where oral transmission presumed be significant factor. By analyzing these recent cases, our research aims provide deeper understanding dynamics during its Colombia essential not only improving management and treatment strategies but also...
Colombia began using artemisinin-based combination therapies for the treatment of uncomplicated Plasmodium falciparum malaria in 2006. It is necessary to implement resistance surveillance antimalarial drugs order promptly detect changes parasite susceptibility. The aim this study was establish a susceptibility baseline P. artemether-lumefantrine three monitoring tools.Patients with treated underwent clinical and parasitological follow-up over 28 days. Ex vivo test performed microtest...
Resistance to anti-malarial drugs is associated with polymorphisms in target genes and surveillance for these molecular markers important detect the emergence of mutations drug resistance signal recovering sensitivity anti-malarials previously used.The presence Plasmodium falciparum chloroquine sulfadoxine-pyrimethamine was evaluated by Sanger sequencing, 85 P. day enrollment samples from a therapeutic efficacy study artemether-lumefantrine conducted 2018-2019 Quibdo, Colombia. Samples were...
Plasmodium falciparum has the ability to counter antiparasitic activity of sulphadoxine-pyrimethamine by progressively accumulating mutations in dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) genes. These gradually increase resistance parasite these drugs lead therapeutic failure.To determine frequency associated with sulphadoxine pyrimethamine dhfr dhps genes P. samples from patients three endemic zones Colombia -La Carpa, Guaviare; Casuarito, Vichada; Tierralta Puerto...
Introduction. Plasmodium falciparum is a highly polymorphic parasite, which allows it to evade the host's immune response, spread drug resistance and favours transmission.Objectives. To analyse genetic diversity of P. populations in samples from four endemic localities Colombia.Materials And Methods. 123 blood were collected on filter paper patients with non-complicated malaria during 2002 2004. The genotyped using polymerase chain reaction specific primers for region block 2 msp1 gene 108...
The genetic diversity of Plasmodium falciparum has been one the major obstacles for success anti-malaria drug therapy. It provides parasite an ability to evade host's immune response by generating changes in its antigenic composition and resistance antimalarial drugs.The P.falciparum was characterized 4 Colombian localities through analysis polymorphic genes.Eighty-one samples were obtained from patients with uncomplicated P. malaria screened variants msp1, msp2 (merozoite surface proteins)...
Introducción. Dadas las dificultades del diagnóstico microscópico de la malaria o paludismo en áreas rurales, pruebas rápido constituyen una buena alternativa, por lo que es importante conocer su desempeño.Objetivo. Evaluar el desempeño utilizadas cinco departamentos para al usando reacción cadena polimerasa (PCR) como estándar referencia.Materiales y métodos. Se usaron prueba gota gruesa y, además, se impregnó papel filtro con sangre molecular (PCR), individuos sintomáticos.Resultados....
This study reports a challenging diagnosis of Plasmodium ovale malaria in Colombian citizen returning from Cameroon. Initial microscopy screenings conducted at two private hospitals yielded conflicting results, with the first showing negative smears and second diagnosing P. vivax. Subsequent examinations government laboratories identified ovale, although routine species-specific PCR strategy was negative. confirmation finally obtained when wallikeri primers were used. Although is not...
A case of illegal cattle purchasing is presented. Basque country police submitted six blood samples: three from the allegedly stolen animals and putative mothers. Four polymorphic DNA loci were analyzed to establish parental relationship. From investigation maternity alleged was determined.
Few studies describe the clinical presentations of uncomplicated Plasmodium falciparum malaria in province Córdoba an endemic area northwestern Colombia.Profiles patients with were described from two twons Córdoba, Tierrata and Puerto Libertador, based on clinical, epidemiological laboratory variables.Patients examined according to standard WHO/PAHO protocols for assessment antimalarial drug efficacy. Clinical data parasitological information was collected as well. A multiple correspondence...
<h3>Introduction</h3> Since 2000 Digital Mammography (DM) has been commercially available and implemented in many breast cancer screening programs. Although information suggests that differences, if exist, are low, complete evaluation of DM is needed to perform a more accurate balance risks benefits these Our purpose was compare tumour characteristics between cancers detected with Screen-Film (SFM) DM, evaluate changes on positive predictive values (PPVs), for further assessments, invasive...