A5049427673- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Ginseng Biological Effects and Applications
- Mycorrhizal Fungi and Plant Interactions
- Plant and fungal interactions
- Microbial Natural Products and Biosynthesis
- Food Quality and Safety Studies
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Agriculture, Soil, Plant Science
- Legume Nitrogen Fixing Symbiosis
- Industrial Vision Systems and Defect Detection
- Insect and Pesticide Research
- Nutrition, Health and Food Behavior
- Plant pathogens and resistance mechanisms
- Cavitation Phenomena in Pumps
- Biocrusts and Microbial Ecology
- Surface Roughness and Optical Measurements
- Thin-Film Transistor Technologies
- Echinoderm biology and ecology
Dong-A University
2014-2024
Seoul National University
2001-2011
Korea Research Institute of Chemical Technology
2011
Kongju National University
2010
Kansas State University
2008
Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins are harmful to animal and human. In this study, a systematic analysis 17 phenotypes the mutants in 657 genes encoding putative transcription factors (TFs) resulted database over 11,000 (phenome). This provides comprehensive insights into how global significance regulates traits for growth, development, stress response, pathogenesis, toxin production...
Fusarium graminearum is an important fungal pathogen of cereal crops and produces mycotoxins, such as the trichothecenes nivalenol deoxynivalenol. This species may be subdivided into a series genetic lineages or phylogenetic species. We identified strains F. from Republic Korea to lineage, tested their ability produce deoxynivalenol, determined composition structure populations which they were recovered. Based on amplified fragment length polymorphism (AFLP), PCR genotyping, chemical...
ABSTRACT Gibberella zeae , a major cause of cereal scab, may be divided into two chemotypes based on production the trichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned sequenced gene cluster for trichothecene biosynthesis from each chemotype. G. H-11 is DON producer isolated corn, 88-1 NIV barley. 23-kb 26-kb 88-1, along with unlinked Tri101 genes. Each contained 10 Tri homologues in same order transcriptional directions as those Fusarium sporotrichioides . Between all except...
Summary Fungi capable of sexual reproduction use heterothallic (self‐sterile) or homothallic (self‐fertile) mating strategies. In most ascomycetes, a single type locus, MAT , with two alternative forms ( MAT1‐1 and MAT1‐2 ) called idiomorphs, controls ability. these idiomorphs reside in different nuclei. contrast, ascomycetes carry both nucleus, usually closely linked. An example the latter is Gibberella zeae species that selfing outcrossing. G. devastating cereal pathogen ubiquitous...
Fusarium graminearum (Gibberella zeae) is an important pathogen of wheat, maize, barley, and rice in South Korea, harvested grain often contaminated with trichothecenes such as deoxynivalenol nivalenol. In this study, we examined 568 isolates F. collected from maize at eight locations Korea. We used amplified fragment length polymorphisms (AFLPs) to identify four lineages (2, 3, 6, 7); lineage 7 was the most common (75%), followed by 6 (12%), 3 2 (1%). The genetic identity among populations...
Peroxisomes are organelles that involved in a number of important cellular metabolic processes, including the β-oxidation fatty acids, biosynthesis secondary metabolites, and detoxification reactive oxygen species (ROS). In this study, role peroxisomes was examined Fusarium graminearum by targeted deletion three genes (PEX5, PEX6, PEX7) encoding peroxin (PEX) proteins required for peroxisomal protein import. PEX5 PEX7 mutants were unable to localize fluorescently tagged targeting signal type...
Abstract Bacterial-fungal interactions are widely found in distinct environments and contribute to ecosystem processes. Previous studies of these have mostly been performed soil, only limited aerial plant tissues conducted. Here we show that a seed-borne pathogenic bacterium, Burkholderia glumae (Bg), an air-borne fungus, Fusarium graminearum (Fg), interact promote bacterial survival, fungal dispersal, disease progression on rice plants, despite the production antifungal toxoflavin by Bg. We...
The velvet genes are conserved in ascomycetous fungi and function as global regulators of differentiation secondary metabolism. Here, we characterized one the genes, designated FgVelB, plant-pathogenic fungus Fusarium graminearum, which causes fusarium head blight cereals produces mycotoxins within plants. FgVelB-deleted (ΔFgVelB) strains produced fewer aerial mycelia with less pigmentation than those wild-type (WT) during vegetative growth. Under sexual development conditions, ΔFgVelB no...
Estrogen, a key sex hormone in humans and other mammals, regulates the female reproductive system has important medicinal applications. It enters environment through sources such as animal feces medical waste. is structurally similar to mycotoxin zearalenone produced by homothallic fungus <i>Fusarium graminearum</i>. This responsible for causing Fusarium head blight on cereal crops around world. We investigated effects of major estrogen, β-estradiol, development <i>F....
In heterothallic ascomycete fungi, idiomorphic alleles at the MAT locus control two sex pheromone-receptor pairs that function in recognition and chemoattraction of strains with opposite mating types. Gibberella zeae, is rearranged such both are adjacent on same chromosome. Strains G. zeae self-fertile but can outcross facultatively. Our objective was to determine if pheromones retain a role sexual reproduction this homothallic fungus. Putative pheromone precursor genes (ppg1 ppg2) their...
ABSTRACT The sucrose nonfermenting 1 ( SNF1 ) protein kinase of yeast plays a central role in the transcription glucose-repressible genes response to glucose starvation. In this study, we deleted an ortholog from Gibberella zeae characterize its functions by using gene replacement strategy. mycelial growth deletion mutants (ΔGz was reduced 21 74% on diverse carbon sources. virulence ΔGz barley decreased, and expression encoding cell-wall-degrading enzymes reduced. most distinct phenotypic...
Summary Apicidin is a cyclic tetrapeptide produced by certain isolates of Fusarium semitectum and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin‐producing strain (KCTC16676) the filamentous fungus was mutated using an Agrobacterium tumefaciens ‐mediated transformation, resulting in 24 apicidin‐deficient mutants. Three mutants had T‐DNA insertion gene that encodes non‐ribosomal peptide synthetase (NRPS). Results sequence, expression, deletion analyses defined apicidin...
ABSTRACT Acetyl coenzyme A (acetyl-CoA) is a crucial metabolite for energy metabolism and biosynthetic pathways produced in various cellular compartments with spatial temporal precision. Our previous study on ATP citrate lyase (ACL) Gibberella zeae revealed that ACL-dependent acetyl-CoA production important histone acetylation, especially sexual development, but not involved lipid synthesis. In this study, we deleted additional synthetic genes, the synthetases ( ACS genes ACS1 ACS2 ), to...
Yellow rot, caused by an ascomycetous fungus having a distinctive arthroconidial anamorph, is the most destructive disease of cultivated Ganoderma lucidum in Korea, but identity yellow rot pathogen (YRP) remains uncertain. Isolates have been identified as Xylogone sphaerospora (with putative anamorph Sporendonema purpurascens) or Arthrographis cuboidea. Therefore we used morphological features, pathogenicity tests and phylogenetic analyses DNA sequences from nuclear ribosomal genes,...
Fusarium head blight (FHB) caused by the filamentous fungus graminearum is one of most severe diseases threatening production small grains. Infected grains are often contaminated with mycotoxins such as zearalenone and trichothecences. During survey contamination FHB in rice grains, we found a bacterial isolate, designated BN1, antagonistic to F. graminearum. The strain BN1 had branching vegetative hyphae spores, its aerial long, straight filaments bearing spores. 16S rRNA gene 100% sequence...
Bakanae or foot rot disease is a prominent of rice caused by Gibberella fujikuroi. This may infect plants from the pre-emergence stage to mature stage. In recent years, raising seedlings in seed boxes for mechanical transplanting has increased incidence many seedling diseases; only few varieties have been reported exhibit resistance bakanae disease. this study, we attempted identify quantitative trait loci (QTLs) conferring highly resistant japonica variety Wonseadaesoo. A primary QTL study...
Carotenoids are a structurally diverse class of terpenoid pigments that synthesized by many microorganisms and plants. In this study, we identified five putative carotenoid biosynthetic genes from the ascomycete Gibberella zeae (GzCarB, GzCarO, GzCarRA, GzCarT, GzCarX). HPLC showed fungus produces two carotenoids: neurosporaxanthin torulene. We deleted individually to determine their functions. GzCarB, GzCarT were required for biosynthesis, but deletion GzCarX or GzCarO (DeltagzcarX...