Obesity and overnutrition during pregnancy affect fetal programming of adult disease. Children born after maternal bariatric gastrointestinal bypass surgery (AMS) are less obese exhibit improved cardiometabolic risk profiles carried into adulthood compared with siblings before (BMS). This study was designed to analyze the impact weight loss on methylation levels genes involved in pathways BMS AMS offspring. Differential analysis between a sibling cohort 25 (2–25 y-old) offspring from 20...

The objective of this study was to determine whether Acylation Stimulating Protein (ASP) is generated in vivo by human adipose tissue during the postprandial period. After a fat meal, samples from 12 subjects were obtained (up 6 h) an arterialized hand vein and anterior abdominal wall that drains tissue. Veno-arterial (V-A) gradients across subcutaneous bed calculated. data demonstrate ASP produced (positive V-A gradient) with maximal production at 3–5 h postprandially. plasma...

Abstract Acylation stimulating protein (ASP) is a 14 kDa plasma which causes in vitro triacylglycerol synthesis human adipocytes and fibroblasts to increase substantially. ASP was found stimulate adipose tissue microsomal glycerophosphate acyltransferase diacylglycerol activities by 23% 90%, respectively. However, phosphatidate phosphohydrolase activity showed no activity, nor did acyl‐CoA synthetase activity. Moreover, not decrease the apparent K m of (DGAT), but rather increased its V max...

Massive liver resection and small-for-size transplantation pose a therapeutic challenge, due to increased susceptibility of the remnant/graft ischemia reperfusion injury (IRI) impaired regeneration. We investigated dual role complement in IRI versus regeneration mice. Complement component 3 (C3) deficiency inhibition with receptor 2–complement 1–related protein y (CR2-Crry, an inhibitor C3 activation) provided protection from hepatic IRI, while also following partial hepatectomy (PHx),...

Objective: Ghrelin [acylated (AG) and nonacylated (NAG)] has been shown to play a pivotal role in the regulation of food intake insulin sensitivity. It is presently unclear whether variation sensitivity related AG NAG levels obese individuals. To address this issue, we determined insulin-sensitive overweight or (ISO) insulin-resistant (IRO) individuals display different total ghrelin (TotG), AG, profiles during euglycemic/hyperinsulinemic clamp (EHC). Design: Eighty-nine nondiabetic...

Acylation-stimulating protein (ASP) is a potent lipogenic produced by adipocytes. In vitro studies have shown that ASP increases triglyceride synthesis and glucose transport in both murine human Our initial study indicated complement C3-deficient (−/−) mice (and, therefore, deficient) demonstrated altered dietary postprandial clearance. the present we examined phenotype of female longitudinally on different diets. Female C3(−/−) low (10% energy) high (40% fat diets displayed an average...

We have previously shown that in normolipidemic healthy adults, plasma acylation stimulating protein (ASP) increases postprandially and is produced vitro by cultured differentiated human adipocytes.The present studies were undertaken to examine the influence of specific components on endogenous ASP production results demonstrate neither glucose nor fatty acids (over a wide range concentrations) had any s u b stantial effect production.Insulin increased up 2-fold (208% ?IS%, P <...

Abstract Acyl‐ghrelin (AG), desacyl‐ghrelin (DAG) and obestatin are all derived from the same gene transcript; however their plasma levels do not necessarily change in parallel. The influence of these peptides towards development obesity direct effects on adipocyte physiology has been thoroughly investigated. This study was designed to evaluate ghrelin family preadipocyte proliferation, differentiation lipid glucose metabolism 3T3‐L1 cells. 3T3 cells were treated with physiological peptide...

Prevalence of obesity is increasing to pandemic proportions. However, obese subjects differ in insulin resistance, adipokine production and co-morbidities. Based on fasting plasma analysis, were grouped as Low Acylation Stimulating protein (ASP) Triglyceride (TG) (LAT) vs High ASP TG (HAT). Subcutaneous (SC) omental (OM) adipose tissues (n = 21) analysed by microarray, biologic pathways lipid metabolism inflammation specifically examined.LAT HAT groups matched age, obesity, insulin, glucose,...

Aims: Cardiac dysfunction can be a fatal complication during severe sepsis. The migration of neutrophils is significantly impaired We sought to determine the role trimetazidine (TMZ) in regulation neutrophil heart mouse model sepsis and endotoxemia, identify mechanism whereby TMZ confers survival advantage. Methods Results: C57/BL6 mice were 1) injected with LPS followed by 24-hour administration, or 2) treated (20 mg/kg/day) for 1 week post cecal ligation puncture (CLP) operation....

Dilated cardiomyopathy (DCM) induced chronic heart failure is a life-threatening disease worldwide. Long non-coding RNAs (lncRNAs) are potential new therapeutic targets and may provide pathophysiological mechanisms for development of DCM.Microarray assays in 14 DCM 10 control human samples identified 313 significantly differentially expressed lncRNAs. SiRNAs were used to explore the function specific lncRNAs (RP11-544D21.2 XLOC_014288) cardiac myocytes, fibroblasts microvascular endothelial...

Acylation-stimulating protein (ASP) acts as a paracrine signal to increase triglyceride synthesis in adipocytes. ASP administration results more rapid postprandial lipid clearance. In mice, C3 (the precursor ASP) knockout deficiency and leads reduced body fat leptin levels. The protective potential of against obesity involvement the pathway were examined ob/ob C3(−/−) double mice (2KO). Compared with age-matched 2KO had delayed fatty acid clearance; associated decreased weight (4–17 weeks...

Ghrelin is a novel peptide that has been isolated from human and rat stomach tissues. Despite its known stimulatory effects on appetite eating behavior, little information available regarding relationship with energy expenditure in normal-weight humans. To address this issue, we examined the between serum ghrelin resting metabolic rate (RMR), thermic effect of food (TEF), fasting postprandial respiratory quotient, physical activity level, peak aerobic capacity (VO2 peak), intake,...

Acylation-stimulating protein (ASP) increases triglyceride (TG) storage (fatty acid trapping) in adipose tissue and plays an important role postprandial TG clearance. We examined the capacity of ASP insulin to stimulate activity lipoprotein lipase (LPL) trapping LPL-derived nonesterified fatty (NEFA) 3T3-L1 adipocytes. Although increased total LPL (secreted cell-associated; P < 0.001) adipocytes, moderately stimulated secreted (P = 0.04; 5% activity). Neither hormone translocation from...

Acylation-stimulating protein (C3adesArg/ASP) is an adipokine that acts on its receptor C5L2 to stimulate triglyceride (TG) synthesis in adipose tissue. The present study investigated ASP levels mouse models of obesity and leanness the effect deficiency C3 knockout (C3KO) mice tissue morphology. Plasma wild-type (WT) correlated positively with plasma nonesterified fatty acids (NEFA) ( R = 0.664, P &lt; 0.001) total cholesterol 0.515, 0.001). was increased by 85% obese ob/ob leptin-deficient...

Objective . To evaluate leptin and adiponectin as biomarkers of metabolic syndrome (MS) risk factors even in nonobese children/adolescents. Methods Serum leptin, adiponectin, leptin:adiponectin ratio, lipids, glucose, insulin concentrations well body size parameters pubertal development were evaluated a large population Chinese children/adolescents (<mml:math...

Acylation stimulating protein (ASP) is a potent stimulator of triglyceride synthesis in adipocytes. In the present study, we have examined effect an ASP functional knockout (ASP(−/−)) on lipid metabolism male mice. both young (14 weeks) and older (26 mice there were marked delays postprandial clearance (80% increase at 14 weeks 120% 26 versus wild type (+/+)). Postprandial nonesterified fatty acids also increased ASP(−/−) miceversus ASP(+/+) by 37% (low fat 10% Kcal) 73% (high 40% diets,...

Acylation-stimulating protein (ASP) acts as a paracrine signal to increase triglyceride synthesis in adipocytes. In mice, C3 (the precursor ASP) knock-out (KO) results ASP deficiency and leads reduced body fat leptin levels yet they are hyperphagic. the present study, we investigated mechanism for this energy repartitioning. Compared with wild-type (WT) male female C3(-/-) ASP-deficient mice had elevated oxygen consumption (VO2) both active (dark) resting (light) phases of diurnal cycle:...