Abstract Since biofilms show strong resistance to conventional disinfectants and antimicrobials, control of initial bacterial adhesion is generally accepted as one the most effective strategies for preventing biofilm formation. Although electrical methods have been widely studied, specific properties cathodic, anodic, block currents that influence detachment inactivation remained largely unclear. This study investigated role electric in bacteria adhered an electrode surface. A real‐time...

This study investigated the application of electrical potentials to an electroconductive feed spacer (ECFS) as a tool for controlling biofouling in lab-scale cross-flow membrane system. When ECFS was electrically polarized 30 min after 24 h occurrence, 33–44% permeate flux recovered without any damage membrane. recovery can be explained by effective detachment attached bacteria or biofilms on surface well ECFC. Overall, results this suggest that with proper potential is method control...

The process of controlling bacterial adhesion using an electric current deserves attention because its ease automation and environmentally friendly nature. This study investigated the role currents (negative, positive, alternating) for preventing Pseudomonas aeruginosa achieving inactivation. Indium tin oxide (ITO) film was used as a working electrode to observe inactivation under polarization. Electric types were classified into negative, alternating current. acted cathode or anode by...

Brucella spp. is the causative agent of brucellosis, one worldwide diseases. The pathogen infects humans and animals mainly through digestive or respiratory tract. Therefore, induction mucosal immunity required as first line defense. In this study, three abortus recombinant proteins, malate dehydrogenase (rMdh), outer membrane proteins (rOmp) 10 19 were loaded in mucoadhesive chitosan nanoparticles (CNs) systemic investigated after intranasal immunization BALB/c mice. These antigens also...

Application of an electric field (alternating or cathodic polarization) has been suggested as a possible mean controlling biofilm development. Bacteria on anodically polarized surface were shown to be active and highly motile when compared with nonpolarized condition, but no quantitative information bacterial motion reported. This study investigated the effects environmental conditions (current density ionic strength) translational P. aeruginosa PAO1 cells under anodic using tracking method....

Abstract Carbapenems are broad-spectrum antibiotics widely used for the treatment of human infections caused by multidrug-resistant (MDR) Gram-negative bacteria. However, emerging carbapenemase-producing Enterobacterales (CPE) rising as a public threat to and animal health. We screened clinical bacterial isolates from 241 dogs 18 cats hospitalized at Veterinary Medical Teaching Hospital, Seoul National University, 2018 2020 carbapenemase production. In our study, 5 strains...

Infection with Brucella abortus causes contagious zoonosis, brucellosis, and leads to abortion in animals chronic illness humans. Chitosan nanoparticles (CNs), biocompatible nontoxic polymers, acts as a mucosal adjuvant. In our previous study, B. malate dehydrogenase (Mdh) was loaded CNs, it induced high production of pro-inflammatory cytokines THP-1 cells systemic IgA BALB/C mice. this the time-series gene expression analysis nasal-associated lymphoid tissue (NALT) performed identify...

Non-tuberculous mycobacteria (NTM) are ubiquitous microorganisms that have the potential to cause disease in both humans and animals. Recently, NTM infections rapidly increased South Korea, especially urbanized areas. However, distribution of species antibiotic resistance profile environmental sources not yet been investigated. Therefore, we analyzed soil within urban areas Korea. A total 132 isolates were isolated from samples 1 municipal animal shelter 4 area parks. Among isolates, 105...

Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne’s disease, a chronic emaciating disease ruminants that causes enormous economic losses to bovine industry, globally. However, there are still remaining clues be solved in pathogenesis and diagnosis disease. Therefore, an vivo murine experimental model was tried understand responses early stage MAP infection by oral intraperitoneal (IP) routes. In size, weight spleen liver were increased IP group compared...

Since recognizing the interaction between Brucella and host cells is crucial to elucidation of infectious process, researches have prioritized investigation genes related pathogenicity. To demonstrate roles genes, RAW 264.7 were infected with abortus wild-type mutant strains (generated using transposon mutagenesis), after which different transcriptional responses determined microarray. Following infection, enhanced strategies for intracellular survival, such as down-regulation associated...

Among various vaccines against Actinobacillus pleuropneumoniae, subunit using recombinant proteins of ApxI, ApxII, and ApxIII as vaccine antigens have shown good efficacy in terms safety protection. Therefore, are being applied worldwide the development new is actively conducted. To evaluate vaccines, it important to measure immune responses each Apx toxin separately. However, cross-reactivity antibodies makes difficult specific reactivity toxin. In present study, antigen regions among...

Abstract Johne’s disease (JD) caused by Mycobacterium avium subsp. paratuberculosis (MAP) is a chronic, wasting infectious in ruminants that causes enormous economic losses to the dairy and beef cattle industries. Understanding mechanism of persistency MAP key produce novel ideas for development new diagnostic methods or prevention techniques. We sought interactions between host using epithelial passage model, which mimic initial stage infection. From transcriptomic analysis bovine immune...

Thermal conditions are an important environmental factor in maintaining healthy pigs because they affect feed intake, growth efficiency, reproduction and immune responses pigs. RAVI, a regenerative far-infrared heating system, can effect pig production by emitting optimal wavelength. Far-infrared radiation has been reported to increase microvascular dilation vascular flow volume. The purpose of this study was evaluate the immunobiological differences between raised with RAVI system gasoline...

Research has been undertaken to understand the host immune response Brucella canis infection because of importance disease in public health field and clinical field. However, previous mechanisms governing this have not elucidated. Therefore, vitro models, which mimic vivo route using a canine epithelial cell line, D17, macrophage, DH82, were established determine these by performing an analysis transcriptomes cells. In study, coculture model was constructed D17 line DH82 transwell plate....

New coronavirus, SARS-CoV-2, named as COVID-19 was identified and caused the global pandemic in 2020. Recent challenging issues include providing users with non-invasive easy-to-use diagnostic devices identifying infected to prevent spread of quick possible. In this study, a new device that integrates porous microneedles (MNs) paper-based immunoassay biosensor developed for rapid antibody detection. The biodegradable MNs made polylactic acid (PLA) were fabricated used extract interstitial...