A5063472622- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Muscle Physiology and Disorders
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- NF-κB Signaling Pathways
- Photoreceptor and optogenetics research
- Extracellular vesicles in disease
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Gene Regulatory Network Analysis
- Genetics and Neurodevelopmental Disorders
- Muscle activation and electromyography studies
- Retinal Development and Disorders
- Cell Image Analysis Techniques
- Nanoplatforms for cancer theranostics
- Adipose Tissue and Metabolism
- Hearing Loss and Rehabilitation
- MicroRNA in disease regulation
- Hearing, Cochlea, Tinnitus, Genetics
- Neural dynamics and brain function
- Signaling Pathways in Disease
Stomatology Hospital
2024
Sun Yat-sen University
2024
Jinan University
2024
Karolinska Institutet
2012-2021
Institute of Molecular Biology and Biophysics
2013
Spiral ganglion (SG) neurons of the cochlea convey all auditory inputs to brain, yet cellular and molecular complexity necessary decode various acoustic features in SG has remained unresolved. Using single-cell RNA sequencing, we identify four types neurons, including three novel subclasses type I II provide a comprehensive genetic framework that define their potential synaptic communication patterns. The connectivity patterns with inner hair cells electrophysiological profiles suggest they...
Abstract Proprioceptive neurons (PNs) are essential for the proper execution of all our movements by providing muscle sensory feedback to central motor network. Here, using deep single cell RNAseq adult PNs coupled with virus and genetic tracings, we molecularly identify three main types (Ia, Ib II) find that they segregate into eight distinct subgroups. Our data unveil a highly sophisticated organization discrete input channels spatial distribution, innervation patterns molecular profiles....
Abstract Somatic sensation is defined by the existence of a diversity primary sensory neurons with unique biological features and response profiles to external internal stimuli. However, there no coherent picture about how this cell states transcriptionally generated. Here, we use deep single analysis resolve fate splits molecular biasing processes during neurogenesis in mice. Our results identify complex series successive specific transcriptional changes post-mitotic that delineate...
The sensation of pain is essential for the preservation functional integrity body. However, key molecular regulators necessary initiation development pain-sensing neurons have remained largely unknown. Here, we report that, in mice, inactivation transcriptional regulator PRDM12, which perception humans, results a complete absence nociceptive lineage, while proprioceptive and touch-sensitive remain. Mechanistically, our data reveal that PRDM12 required neurogenesis activation cascade...
Alzheimer's disease (AD) has been largely considered as an age-related disease, mainly affecting mature or aging adult brain. Recent studies show that AD-associated mutations could impair early life, even during neurodevelopment. However, due to the complex of AD and neurodevelopmental regulations, how in specific genes affect origin neurodevelopment is still under studied. In this study, we investigate App gene impact neurodevelopment, with a focus on NSC dynamics balance between...
The control of all our motor outputs requires constant monitoring by proprioceptive sensory neurons (PSNs) that convey continuous muscle inputs to the spinal network. Yet molecular programs establishment this sensorimotor circuit remain largely unknown. transcription factor RUNX3 is essential for early steps PSNs differentiation, making it difficult study its role during later aspects specification. Here, we conditionally inactivate Runx3 in after peripheral innervation and identify...
The principle by which unmyelinated primary sensory neurons transducing thermal, itch and pain perception are specified in early development is unknown. These classes of diversify from a common population late-born neurons, initiate expression Runt homology domain transcription factor RUNX1 the nerve growth receptor TrkA. Here, we report that signals emanating within mouse dorsal root ganglion mediated partly early-born destined to myelinated phenotype participate fating + /TrkA neurons....
Abstract Developmental cell death plays an important role in the construction of functional neural circuits. In vertebrates, canonical view proposes a selection surviving neurons through stochastic competition for target-derived neurotrophic signals, implying equal potential to compete. Here we show alternative fitness that is defined by specific neuronal heterogeneity code. Proprioceptive sensory will undergo and those survive exhibit different molecular signatures are regulated retinoic...
Abstract Hypothyroidism due to THRA1 (gene coding for thyroid hormone receptor α1) mutation-mediated Resistance Thyroid Hormone (RTH) has been recently reported in human and is associated with memory deficits similar those found a mouse model Thra1 mutation mediated RTH ( +/ m mice). Here, we show that short-term treatment of mice GABA A antagonist pentylenetetrazol (PTZ) completely durably rescues their performance. In the CA1 region hippocampus, improvement increased long-term potentiation...
Neurotrophins are key players of neural development by controlling cell death programs. However, the signaling pathways that mediate their selective responses in different populations neurons remain unclear. In mammalian cochlea, sensory differentiate perinatally into type I and II both expressing TrkB TrkC, which bind respectively brain-derived neurotrophic factor (BDNF) neurotrophin-3 (NT3). How these two neuronal respond differentially to neurotrophins remains unknown. Here, we report rat...