Natural killer (NK) cell immunotherapy has gained attention as a promising strategy for treatment of various malignancies. In this study, we used genome-wide CRISPR screen to identify genes that provide protection or susceptibility NK cytotoxicity. The confirmed the role several in regulation, such involved interferon-γ signaling and antigen presentation, well encoding receptor ligands B7-H6 CD58. Notably, gene TMEM30A , CDC50A–beta-subunit flippase shuttling phospholipids plasma membrane,...

BACKGROUND: AFP serum levels are considered as diagnostic and specific for hepatocellular carcinoma (HCC) in patients with liver cirrhosis (LC).
 AIM: This study aimed to examine the value of distinguishing HCC from LC, analyse potential correlation between disease stages.
 MATERIAL AND METHODS: Fifty LC fifty were included this study. The majority males, while HBV aetiology was dominant.
 RESULTS: Significant differences detected AST, ALT, GGT, bilirubin, AP. Patients had...

Trivalent chromium is an essential trace element for humans and animals it the final product of hexavalent reduction in cell.It integral part glucose-tolerance factor, which increases systemic impact insulin.The aim this study was to analyse effect trivalent on selected biochemical parameters Japanese quails.Birds were divided into three groups: group I (control group, n=12), II (treated with chromium, n=12) III n=12).During 20 days follow-up, control experimental groups quails had identical...

Cadmium is a heavy metal, toxic even in trace amounts and its biological function the human body has not been described to date. It assumed that cadmium manifests dose-dependent genotoxic cytotoxic effects on many organs tissue types. In this study, we have analyzed biochemical parameters serum of Japanese quails (Coturnix japonica) after chronic vivo exposure cadmium. Adult animals were exposed form CdCl2 dissolved water (0.20 mg/L) for 20 days. Significant differences between controls...

Studies based on biochemical and hematological parameters in fish are significant monitoring of health early detection pathological conditions.This research obtained values serum electrolytes crucian carp (n = 15) common 13).Significantly different K + , Cl -and Ca 2+ concentration were detected.In there significantly higher lower compared to the carp.Hematological differ these two species.In blood PCV, hemoglobin concentration, RBC MCV notably MCHC WBC obtained, carp.This preliminary is...

Dexamethasone is synthetic corticosteroide which influences the metabolism of glucose, ion transport in organisms and values certain hematological parameters.In this research, dexamethasone was applied musculus gluteus maximus Wistar rat species form phosphate sodium solution 1 mL ampoules.Each ampoule contains 4 mg dexamethasone, administered each 24 hours days period.Glucose potassium concentrations serum, platelet count whole blood were observed.After experiment statistical processing...

Abstract Emperipolesis is considered a physiological phenomena often present in various pathophysiological conditions, but its etiology still unknown. In this study, we analyzed the number of megakaryocytes and percentage emperipoletic cells sternal femoral bone marrow Wistar rats. Five types thrombopoiesis lineage (megakaryoblasts, promegakaryocytes - acidophilic, basophilic thrombocytogenic) were determined. Except for megakaryocytes, significant differences found thrombopoietic marrow. A...

Imatinib mesylate, a selective BCR-ABL tyrosine kinase inhibitor, has been well established as the standard of care for chronic myeloid leukaemia patients.In this study, we compared clinical outcomes patients who received first-line Glivec (Group 1) with generic imatinib 2) in Bosnia and Herzegovina three years follow-up therapy.At 24 months therapy, achievement complete cytogenetic response major molecular were comparable between studied groups (CCyR was 69% vs. 70%, respectively; MMR 54%...

The total number of hemocytes and their morphological characteristics were analyzed in Helix pomatia Linnaeus, 1758, by light microscopy during different phases hibernation. Three types identified: hyalinocytes, agranulocytes, granulocytes. Total decreased significantly Most intensive changes cell size, morphology, appearance special structures granulocytes happened the hibernation period itself. Termination coincides with very important granulocyte transformation specific gigantic...

The identification of mutually exclusive somatic mutations shared among myeloproliferative neoplasm (MPN) subtypes has provided a powerful tool for studying disease evolution. Clinical features, gene mutations, and survival over 18 years were analyzed in MPN patients. One hundred thirty-eight patients subcategorized according to subtypes: essential thrombocythemia (ET, n = 41), polycythemia vera (PV, 56), primary myelofibrosis (PMF, 10), unclassified (MPN-U, 31). Patient characteristics...

In developing countries, TKI is limited and many patients have delayed start of therapy.Superiority nilotinib in treatment not well studied.We previously recognized the possible superior effect nilotinib, decided to analyse long-term effects.In this study we presented outcomes 70 CML categorized into Group 1 (n= 31, front-line nilotinib) 2 39, imatinib, second-line nilotinib).CCyR MMR at 24 months on were higher (88% vs. 75% 81% 59%, respectively).We further subcategorized also compared...

Abstract The aim of this study was to analyze the values biochemical parameters in patients with diabetic ketoacidosis and ketonuria. In prospective comparative conducted at Clinical Center University Sarajevo, hundred both genders diabetes mellitus were enrolled. Newly diagnosed complications like acute (n = 50) ketonuria included compared. these analyzed. We found that mean pH, base excess, hydrogencarbonate, sodium, glucose, urea creatinine concentrations significantly different compared...

B u çalışmada NaOCl çözeltisinin Gökkuşağı balığı Oncorhynchus mykiss (Walbaum, 1792) kanı üzerindeki hematolojik ve biyokimyasal etkileri analiz edildi.Suyun daha önceden klorlanması ile tedavi üç gün sürdü.K + , Ca 2+ Cl -, proteinler, glikoz konsantrasyonunda önemli farklılıklar tespit edildi en büyük varyasyonlar kreatinin konsantrasyonu için bulundu (P <0.05) PCV, Hb konsantrasyonu, RBC, WBC, MCH, MCHC, LYM (%), SEG (%) NEU (%

Total number and types of hemocytes were analysed in hemolymph Roman snail (Helix pomatia L.).Significant differences the during season at different locations identified.The average ranged from 154 to 288 per 10 μL hemolymph.The highest was recorded snails higher altitudes.Significant intergroup between all sites most numerous granulocytes, followed by agranulocytes, while hyalinocytes least common post-hibernation period.Post hoc analysis showed certain intragroup for except comparison wet...

Purpose:The aim of this study was to analyze the predictive potential selected biochemical and hematological parameters in patients with gout from Bosnia Herzegovina, determine most common comorbidities related gout.Methods: Forty (17 males 23 females) hospital municipality Novi Grad, Sarajevo were included present study.We analyzed (uric acid, urea, creatinine, CRP), ESR, WBC as a parameter.The Shapiro-Wilk test Spearman correlation coefficient used for normality analysis,...

e18549 Background: In this study we examined the long-term real life clinical outcomes of patients receiving front- line nilotinib and who switched from branded or generic imatinib to (second-line therapy) in Bosnia Herzegovina. Methods: Patients CML-CP (n = 70) started their TKI treatment period June 2005 August 2016 were included multicentre retrospective cohort study. categorized as: Group 1 31) contained front-line (300 mg twice daily) 2 39) consisted with (Glivec imatinib) then (400 600...