A5060252152- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Orthopedic Surgery and Rehabilitation
- Bone fractures and treatments
- Shoulder Injury and Treatment
- Angiogenesis and VEGF in Cancer
- Shoulder and Clavicle Injuries
- Sarcoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Spectroscopy and Chemometric Analyses
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Optical Imaging and Spectroscopy Techniques
- Digital Imaging for Blood Diseases
- Biosensors and Analytical Detection
- Foot and Ankle Surgery
- Bone Tumor Diagnosis and Treatments
- Augmented Reality Applications
- Digital Holography and Microscopy
- PI3K/AKT/mTOR signaling in cancer
- Hemoglobin structure and function
- Surgical Simulation and Training
- Hip and Femur Fractures
- Cancer-related molecular mechanisms research
University of Science and Technology of China
2016-2022
Shanghai Tenth People's Hospital
2017-2021
Tongji University
2017-2021
Hefei University
2020-2021
Ruijin Hospital
2016-2019
Integrated Chinese Medicine (China)
2015-2019
Shanghai Jiao Tong University
2014-2018
Nanjing University of Aeronautics and Astronautics
2016
Metformin is an oral drug that has been widely used to treat type 2 diabetes mellitus. Interestingly, accumulated evidence indicate metformin may reduce the risk of cancer in patients with and inhibit tumor cell growth survival numerous malignancies, including osteosarcoma (OS) cells. In present study, we aimed investigate effects on proliferation, migration, invasion, sphere formation OS MG63 cells vitro. suppressed proliferation a dose- time-dependent manner markedly blocked...
The aim of this study was to provide a basis for the theory that combination conventional chemotherapy and immunotherapy would be an effective treatment osteosarcoma. Here, expression programmed death ligand 1 (PD-L1) in 26 clinical osteosarcoma tissue samples collected before after analyzed. effects cells treated with doxorubicin, chemotherapeutic agent, on proliferation apoptosis CD8 T lymphocytes were investigated vitro. Thereafter, effectiveness doxorubicin combined anti-PD-L1 antibody...
In this study, we identified microRNAs that regulate the expression of programmed death-ligand 1(PD-L1) in osteosarcoma and investigated their role PD-L1-targeted immunotherapy. MicroRNA sequencing analysis showed PD-L1 is regulated by microRNA-200a U2OS, 143B, K7 cells. MicroRNA-200a overexpression induced upregulation CD8+ T cells co-cultured with microRNA-200a-overexpressing reduced survival, proliferation, secretion granzyme B perforin. The same phenomenon was also observed K7-derived...
Abstract In this study, we identified the multifaceted effects of atezolizumab, a specific monoclonal antibody against PD-L1, in tumor suppression except for restoring antitumor immunity, and investigated promising ways to improve its efficacy. Atezolizumab could inhibit proliferation induce immune-independent apoptosis osteosarcoma cells. With further exploration, found that atezolizumab impair mitochondria cells, resulting increased release reactive oxygen species cytochrome-c, eventually...
Focal adhesion kinase ( FAK ) overexpression is related to invasive and metastatic properties in different kinds of cancers. Target therapy by inhibiting has achieved promising effect some cancer treatments, but its human osteosarcoma not been well studied. In the present study, we analyzed antitumor efficacy PF 562271, an inhibitor, against vitro vivo . Phosphorylated (Y397) was highly expressed primary tumor samples associated with prognosis lung metastasis. 562271 greatly suppressed...
Microenvironment at the metastatic locus usually differs greatly from that present in site of primary tumor formation and it has a significant impact on fate extravasated cancer cells. We compared gene expression signatures tumors lung tumors, identified Thrombospondin-1 (TSP1) as highly up-regulated tumors. Immunohistochemical staining further indicated TSP1 protein was higher to both osteosarcoma xenograft model human clinical samples. mRNA level is significantly associated with Enneking...
In this paper we report the development of a cost-effective, modular, open source, and fully automated slide-scanning microscope, composed entirely easily available off-the-shelf parts, capable bright field fluorescence modes. The X-Y stage is two low-cost micrometer stages coupled to stepper motors operated in open-loop mode. microscope CMOS sensor board lenses placed 4f configuration. system has approximately 1 micron resolution, limited by f/# lenses. compact, measuring just 25×25×30 cm,...
Biological nanoparticles are important targets of study, yet their small size and tendency to aggregate makes heterogeneity difficult profile on a truly single-particle basis. Here we present label-free system called 'Raman-enabled nanoparticle trapping analysis' (R-NTA) that optically traps individual nanoparticles, records Raman spectra tracks particle motion identify chemical composition, size, refractive index. R-NTA has the unique capacity characterize aggregation status absolute...
BACKGROUND:Understanding the nutrient foramina is critical to clinical practice. An insult can be caused by trauma and/or surgical dissection and lead devascularization bad outcomes. Few studies have looked at humerus, no described relative information of humeral foramen related anatomical structures that might located palpable landmarks. In this study, we analyzed features diaphyseal humerus provide a discussion relevance. MATERIAL AND METHODS:We dissected 19 cadavers positions surrounding...
CARCI is a fast confocal Raman imaging system using brightfield images to define the sample ROI, which imaged 5×–10× faster compressive sensing. This allowed 98 yeast cells be in time traditional could only measure 20.
We propose a projective navigation system for fluorescence imaging and image display in natural mode of visual perception. The consists an excitation light source, monochromatic charge coupled device (CCD) camera, host computer, projector, proximity sensor Complementary metal-oxide-semiconductor (CMOS) camera. With perspective transformation calibration, our surgical is able to achieve overall speed higher than 60 frames per second, with latency 330 ms, spatial sensitivity better 0.5 mm both...
Humeral shaft fractures are generally managed with the conventional posterior open reduction and internal fixation (ORIF) or minimally invasive plate osteosynthesis (MIPO). This study was aimed at comparing outcomes of these surgical techniques in terms vascular integrity mid-distal humeral shaft. Twelve upper limbs were harvested from 6 fresh cadavers. ORIF MIPO randomly performed on either side each pair limbs. The axillary artery perfused a latex-lead tetraoxide red solution to visualize...
We propose one-step synthesis of hemoglobin-laden microcapsules (HbMs) by the liquid-driven coaxial flow focusing (LDCFF) method, in which purified hemoglobin (Hb) is fully encapsulated inside photocurable resin with a solid core–shell structure. The polymeric shell constitutes rigid semipermeable membrane prevents leakage Hb and allows oxygen transport. Gas-binding capacity affinity HbMs are comparable to Hb. Solid phantoms (SHMPs) also fabricated using resin-based homogeneously mixed...
Background: Plate fixation using traditional lateral L-shape approach for intra-articular calcaneal fractures is complicated by 30% of wound complications, and the small incision techniques with a tarsal sinus cannot sufficiently address all fragments. A modified combined advantages approaches treatment was developed. Method: This prospective study included 29 patients (13 Sanders type II 16 III) were managed this technique. Calcaneal height, width, length, Bohler’s angle, Gissane angle...
Background: Tumor metastasis causes high mortality in patients with malignancies. In carcinomas, overexpression of high-mobility-group A2 (HMGA2) cancer cells would lead to tumor development and epithelial mesenchymal transition (EMT), promoting metastasis. This study evaluated HMGA2 for its effects on pancreatic (PC). Methods: protein levels were immunohistochemically assessed human PC tissue specimens associations patients' clinicopathological findings. CAPAN 1 after expression was...
Low-resolution Raman provides a simple approach to improve the SNR of hyperspectral images, particularly for consumer grade, noisy detectors.
With the advent of hyperspectral Raman imaging technology, especially rapid and high-resolution schemes, datasets with thousands to millions spectra are now commonplace. Standard preprocessing regression methods such as least squares approaches time consuming require input from highly trained operators. Here we propose a solution this analytic bottleneck through convolutional neural network fully on synthetic data then applied experimental measurements, including cases where complete...
Current flow‐based blood counting devices require expensive and centralized medical infrastructure are not appropriate for field use. In this article we report a streamlined, easy‐to‐use method to count red cells (RBC), white (WBC), platelets (PLT) 3‐part WBC differential through cost‐effective automated image‐based system. The approach consists of using compact, custom‐built microscope with large field‐of‐view record bright‐field fluorescence images samples that diluted single, stable...