Several studies conducted around the world showed substantial vitamin D insufficiency and deficiency among different population groups. Sources of in human body include ultraviolet B (UVB)-light-induced biosynthesis dietary intake, but people’s diets are often poor D. Furthermore, many regions, sun exposure intensity UVB irradiation during wintertime not sufficient for biosynthesis. In Slovenia, epidemiological data about status were investigated through a national Nutrihealth study—an...

Measurement of cardiac troponin in serum is an essential part diagnosing myocardial infarction the emergency department. The guidelines suggest that high-sensitivity techniques should be used for measuring I (cTnI) or T (cTnT). aim our study was to correlate values both troponins, and ascertain which type more agreement with diagnosis. patients were classified into four groups: 43 non-ST-elevation (NSTEMI), 7 ST-elevation (STEMI), 48 Type 2 infarction, 21 control group. A significant...

Summary: Background: Our aim was to evaluate the prognostic value of detectable high-sensitivity cardiac troponin I (hs-cTnI) and ischaemia-modified albumin (IMA) in predicting all-cause death or non-fatal ischaemic events patients with PAD after endovascular revascularisation lower limbs. Patients methods: who underwent successful for chronic limb-threatening ischaemia (CLTI) disabling intermittent claudication (IC) were prospectively included. Pre-procedural levels hs-cTnI IMA measured,...

Introduction Traumatic brain injury (TBI) is often associated with reduced cerebral blood flow and an increased inflammatory response, leading to secondary damage. Stellate ganglion block (SGB) has been shown improve hemodynamics in non-TBI, but its effects TBI are still unclear. Objective This prospective pilot study investigates the of SGB on neuroinflammatory responses patients moderate severe aim evaluating potential as a therapeutic intervention. Methods A prospective, single-center...

Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children healthy children, members same family. The average age group was 10.7 years, while control 9.4 years. Urinary metabolites were quantified via liquid chromatography-mass spectrometry operating multiple reaction...

Folate deficiency is associated with various health issues, including anemia, cardiovascular disease, and birth defects. Low folate intake suboptimal status were found in several countries; however, this topic has not yet been investigated Slovenia. Dietary serum through the nationally representative food consumption study SI.Menu/Nutrihealth. was estimated using a sample of N = 1248 subjects aged 10-74 years, stratified three age groups (adolescents, adults, elderly population), two 24...

Vitamin B12 deficiency poses a health concern, especially in vulnerable populations. Dietary vitamin intake was obtained by two 24 h dietary recalls and food propensity questionnaires representative Slovenian cross-sectional consumption survey, SI.Menu (n = 1248 subjects; 10–74 years). For subgroup of 280 participants, data on serum were available through the Nutrihealth study. The estimated usual population-weighted mean daily intakes 6.2 µg (adults), 5.4 (adolescents), 5.0 (elderly). Lower...

Background: The early detection of ovarian cancer is presently not effective, and it crucial to establish biomarkers for the diagnosis improve survival patients. Materials methods: aim this study was investigate role thymidine kinase 1 (TK1) in combination with CA 125 or HE4 serve as a potential diagnostic cancer. In study, set 198 serum samples consisting 134 tumor patients 64 healthy age-matched controls were analyzed. TK1 protein levels determined using AroCell TK 210 ELISA. Results: A...

Dysfunction of the autonomic nervous system (ANS) often complicates clinical course in patients with acute stroke. The studies function ANS brainstem stroke are scarce. purpose our study was to evaluate early period after and find out whether location influences ANS. We studied heart rate variability (HRV) plasma levels catecholamines 14 eligible medullary (6 patients) non-medullary (8 during first 6 weeks Integrals over low- (LFB; 0.04–0.15 Hz) high-frequency bands (HFB; 0.15–0.40 power...

We compared electrocardiographic abnormalities and plasma norepinephrine concentrations in 40 patients with subarachnoid hemorrhage within the first 24 hours, at 72 after 1 week. In 20 high sinus tachycardia (p less than 0.02) negative T waves 0.01) were more frequent normal concentrations. After only 0.03) was found increased frequency 26 Although had week, we no differences of to norepinephrine. However, QTc prolongation, U waves, ST depression, arrhythmias similar both conclude that,...

In the present study, differences in leptin levels between different groups of male patients presenting with infertility problems and possible correlations clinical, spermiological, histological hormonal characteristics were examined. Two hundred ten partners from infertile couples included study. Based on clinical examination, spermiogram testicular histology results, divided into four groups: 42 men non-obstructive azoospermia, 15 obstructive 68 oligoasthenoteratozoospermia 85...

Background: Autism spectrum disorder (ASD) is a developmental characterized by deficits in social interaction, restricted interest and repetitive behavior. Oxidative stress response to environmental exposure plays role virtually every human disease represents significant avenue of research into the etiology ASD. The aim this study was explore diagnostic utility four urinary biomarkers oxidative stress. Methods: One hundred thirty-nine (139) children adolescents with ASD (89% male, average...

The aim of our study was to compare the total 25(OH)D fraction, bioavailable vitamin and free D fraction in spring fall a group healthy individuals.

Thymidine kinase (TK1) is an enzyme involved in DNA synthesis that leaks into the blood as a result of high cell turnover, particularly case cancer. Serum TK1 activity has been used for prognosis and monitoring leukemia lymphoma patients many years. Here, we describe first clinical results with newly developed TK 210 ELISA from AroCell AB. Sera 124 breast cancer known TNM classification along sera 53 healthy females were analyzed by protein levels 3[H]-deoxythymidine (dThd) phosphorylation...

Abstract Objectives The aim of the present study was to establish population- and laboratory-specific reference intervals (RIs) for Slovenian adult population 24 trace elements (TEs) in blood, plasma erythrocytes evaluate impact gender, age, seafood consumption, smoking habits amalgam fillings on TEs levels. Methods (Mn, Co, Cu, Zn, Se Mo, Li, Be, V, Cr, Ni, Ga, As, Rb, Sr, Ag, Cd, Sn, Cs, Au, Hg, Tl, Pb U) were determined 192 a priori selected blood donors (107 women 85 men, aged 18–65...

Thymidine Kinase 1 (TK1) plays an important role in DNA precursor synthesis and serum TK1 activity has been used as a biomarker for prognosis therapy monitoring of different malignancies. AroCell developed dual monoclonal antibody ELISA determination protein clinical samples. The purpose the study is to validate analytically relation gold standard, [ 3 H]-deoxythymidine (dThd) phosphorylation assay using sera from patients with colorimetric TK 210 was validated by assessment precision,...

Inadequate iron intake and deficiency are recognised as a public health problem in the population at large, particularly specific subpopulations. Dietary was analysed using data of national Slovenian food consumption study, SI.Menu (n = 1248 subjects; 10–74 years), while status evaluated with laboratory analyses blood haemoglobin, serum ferritin, concentration samples, collected Nutrihealth study 280, adults). The estimated daily usual population-weighted mean intakes ranged from 16.0 mg...

Inflammatory response in surgery is associated with the release of cytokines. Many cytokines are produced by macrophages; therefore surgical injuries to liver may have great influence on Ischemia creates tissue injury and contribute cytokine release. A balanced ratio pro- anti-inflammatory important for appropriate immune response; excessive inflammation or hypo-responsiveness can lead post-operative complications. To determine magnitude caused evaluate balance released during operation, we...

Autism spectrum disorder (ASD) is a complex developmental in which communication and behavior are affected. A number of studies have investigated potential biomarkers, including uremic toxins. The aim our study was to determine toxins the urine children with ASD (143) compare results healthy (48). Uremic were determined validated high-performance liquid chromatography coupled mass spectrometry (LC-MS/MS) method. We observed higher levels p-cresyl sulphate (pCS) indoxyl (IS) group compared...

A bstract Background: Basic fibroblast growth factor (bFGF) expression is implicated in proliferative diabetic retinopathy (PDR). The aim of this study was to investigate the association genetic polymorphisms (−553T/A, −834T/A and −921C/G) promoter region bFGF gene with PDR patients type 2 diabetes. second determine whether serum levels are affected by factors. Methods: In cross‐sectional case–control 313 unrelated Caucasians (Slovene population) diabetes mellitus were enrolled: 206 control...

Trace elements, including zinc (Zn) and copper (Cu), are known to play important roles in human health. The present study aimed assess the levels of Zn Cu cord blood maternal milk analyze their association with infant characteristics pregnancy outcomes a Slovenian population mothers neonates recruited within PHIME prospective cohort study. included 324 mothers, but data on both was available for 243 mothers. Questionnaires were used socio-demographic health status lifestyle habits (including...