Thymidine kinase 1 (TK1) is a cellular enzyme involved in DNA precursor synthesis, and its activity has been used as proliferation marker for monitoring malignant diseases. Here, the first time, we evaluated both TK1 protein levels sera from patients with different malignancies.Serum samples myelodysplastic syndrome (MDS, n = 22), breast cancer (n 42), prostate 47) blood donors 30) were analyzed levels, using serum (STK1) assay based on antibodies an that measured [(3)H]-deoxythymidine...

Background: The early detection of ovarian cancer is presently not effective, and it crucial to establish biomarkers for the diagnosis improve survival patients. Materials methods: aim this study was investigate role thymidine kinase 1 (TK1) in combination with CA 125 or HE4 serve as a potential diagnostic cancer. In study, set 198 serum samples consisting 134 tumor patients 64 healthy age-matched controls were analyzed. TK1 protein levels determined using AroCell TK 210 ELISA. Results: A...

Thymidine kinase (TK1) is an enzyme involved in DNA synthesis that leaks into the blood as a result of high cell turnover, particularly case cancer. Serum TK1 activity has been used for prognosis and monitoring leukemia lymphoma patients many years. Here, we describe first clinical results with newly developed TK 210 ELISA from AroCell AB. Sera 124 breast cancer known TNM classification along sera 53 healthy females were analyzed by protein levels 3[H]-deoxythymidine (dThd) phosphorylation...

Abstract Background Thymidine kinase 1 (TK1) is a salvage enzyme involved in DNA precursor synthesis, and its expression proliferation dependent. A serum form of TK1 has been used as biomarker human medicine for many years more recently to monitor canine lymphoma. Canine not cloned studied. Therefore, dog cDNA were expressed, the recombinant enzymes characterized. The cellular forms studied by size-exclusion chromatography level protein was determined using polyclonal monoclonal anti-TK1...

Thymidine Kinase 1 (TK1) plays an important role in DNA precursor synthesis and serum TK1 activity has been used as a biomarker for prognosis therapy monitoring of different malignancies. AroCell developed dual monoclonal antibody ELISA determination protein clinical samples. The purpose the study is to validate analytically relation gold standard, [ 3 H]-deoxythymidine (dThd) phosphorylation assay using sera from patients with colorimetric TK 210 was validated by assessment precision,...

Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) from Abcam. Overall, showed higher sensitivity than Abcam differentiating hematological malignancies (sensitivity 0.77 vs 0.45) well distinguishing sera patients with solid tumors those apparently healthy individuals...

Thymidine kinase 1 (TK1) is a DNA precursor enzyme whose expression closely correlated with cell proliferation and turnover. Sensitive serum TK1 activity assays have been used for monitoring prognosis of hematological malignancies in both humans dogs. Here we describe the development specific sandwich TK1-ELISA quantification protein levels sera from dogs different malignancies. A combination rabbit polyclonal anti-dog antibody mouse monoclonal anti-human was used. Different concentrations...

A 70 year old man having progressive dysphagia, coronary artery disease (CAD) with an Ejection Fraction (EF) of 30% was detected to have a poorly differentiated esophageal malignancy. Immunohistochemistry (IHC) positive for HMB 45 and SOX 10 confirming melanoma. In view advanced age poor cardiac function, surgical treatment not feasible. Palliative metallic stenting performed. Immunotherapy suggested ICI. We present this case in rarity melanoma stress the need evaluation IHC subjects

Thymidine kinase 1 (TK1) is a deoxyribonucleic acid (DNA) precursor enzyme and proliferation biomarker used for prognosis treatment monitoring of breast cancer in humans. The aim was to determine if serum thymidine (sTK1) activity sTK1 protein levels dogs with mammary tumors could be useful veterinary medicine. Serum samples from 20 healthy 27 were analyzed activity, using an [3H]-deoxythymidine (dThd) phosphorylation assay, by immune affinity/Western blot assay. molecular forms acute...

Prostate-specific antigen (PSA) is an established tumour marker for prostate cancer (PCa). Serum thymidine kinase 1 a possible new the detection of PCa. The aim study was to investigate diagnostic value AroCell TK 210 enzyme-linked immunosorbent assay (ELISA) together with free PSA, [-2]proPSA, and Prostate Health Index (PHI) in differentiating PCa from benign urological conditions.Serum samples 140 patients PSA values range between 2 10 µg/L were collected at Ljubljana University Medical...

Thymidine kinase 1 (TK1), involved in DNA precursor synthesis, is used as a serum biomarker cancer diagnostics both human and veterinary medicine. We investigated the utility of TK1 protein (TK1p) activity (TK1a) determinations for prognosis monitoring canine hematological malignancies. The combination TK1p or TK1a with C-reactive (CRP) was also investigated. Serum samples from 51 client-owned dogs naive malignancies 149 healthy subjects were included. levels determined using prototype...

In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. cell extracts, TK1 increased twofold with µM Dox both lines, while culture media 5 10 only case cells. conclusion, reveals that can measure intra- extracellular apparently related to mechanism cytotoxicity anti-cancer agents.

Abstract Background: Diffuse large-cell B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin’s lymphomas (NHL) that accounts for approximately 25% patients. Earlier studies demonstrated prognostic value serum thymidine kinase 1 (STK1) activity determinations in different hematological malignancies. To date, STK1 protein concentration patients with DLBCL treated R-CHOP has not been investigated. The purpose this retrospective study was to determine high TK levels on Overall...

Ovarian cancer is the eighth most common cause of death in women. One major concerns almost two-thirds cases are typically diagnosed late stage as symptoms unspecific early ovarian cancer. It known that combination TK1 protein with CA 125 or HE4 showed better performance than either them alone. That why, aim study was to investigate whether TK1-specific activity (TK1 SA) could function a complement marker for early-stage diagnosis

Autoimmune hepatitis (AIH) is a clinical condition of progressive necroinammatory disease the liver. Its association with autoimmune hemolytic anemia (AIHA) rare. It usually diagnosed based on presence presentations, laboratory ndings certain antibodies and characteristic histology. We report young female aged 27 years jaundice, loss appetite which was as AIH AIHA. Treatment for both indications necessary to prevent further progression there good response steroid therapy.

Abstract Background Tissue polypeptide-specific antigen (TPS) has been described as a potentially useful serum marker of tumor activity in adult epithelial tumors such breast, ovarian, prostate, and other malignancies. Here we developed analytically validated Chemiluminescence immunoassay (CLIA) for TPS on the CIA series analyzer FOSUN collaboration with Jiangsu ZECEN Biotech Co., Ltd (Jiangsu Beijing, China). Methods The TPS-CLIA relies two monoclonal antibodies generated against peptides...

Abstract Background: Thymidine Kinase 1 (TK1) is a cytosolic enzyme that plays an important role in DNA precursor synthesis and TK1 activity serum established biomarker for prognosis treatment monitoring, particularly haematological malignancies. The AroCell TK 210 ELISA determines protein levels, contrast to activity, it has demonstrated superior sensitivity distinguishing between subjects with solid tumours controls. Pro PSA, free PSA the prostate health index (PHI) have been proposed as...

Autoimmune pancreatitis (AIP) is a rare form of with mild attacks abdominal pain, pancreatic enlargement, and changes the duct (PD) CBD. We present case AIP mimicking cholangiocarcinoma. A 50 year old female presented 5 days scleral icterus, pruritus, malaise fatigue. She denied nausea, vomiting, weight loss, or pain. Vitals were stable physical exam was benign. Labs showed T. Bili 8.14, AP 562, GGT 286, AST 264, ALT 427, Amylase 46, Lipase 18, ANA negative, pANCA < 1:20, ASMA 9 (negative)....

Purpose: Herpes simplex (HSV) esophagitis is frequently seen in immunocompromised patients. It responds to treatment with either acyclovir or valcyclovir. We describe a case of HSV intractable symptoms despite and endoscopic resolution, which responded an empiric course gabapentin. Results: A 75 year-old male- post liver transplant for hepatitis C on tacrolimus therapy- presented progressive odynophagia, dysphagia, atypical chest pain, anorexia 25 lb weight loss. He had history herpes...

Upper gastrointestinal (UGI) bleeding has many etiologies. We present a unique case of UGI secondary to an extraluminal source.FigureA 79 year old Caucasian male presented twenty days after abdominal aortic aneurysm (AAA) repair, with one-day history several episodes black tarry stools, fatigue and dizziness. He denied pain, nausea or vomiting. Vital signs were stable. Abdominal exam was completely benign. Rectal revealed stools. started on IV proton pump inhibitor. Labs showed: Hb = 8.7,...

Abstract TK activity measurements have been used for many years to monitor cancer disease activity. However, what the blood concentrations of TK1 protein reflect is still uncertain. A immunoassay, AroCell 210 ELISA, has developed based on specific monoclonal antibodies against C-terminal region TK1. We utilized this assay in a cohort patients with Hodgkin lymphoma (HL) before and after conventional treatment. serum or plasma were measured using ELISA. For comparison, large number other...