A5046409209- DNA Repair Mechanisms
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Molecular Biology Techniques and Applications
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Biochemical and Structural Characterization
- Escherichia coli research studies
- Nanofabrication and Lithography Techniques
- Molecular Junctions and Nanostructures
- Amoebic Infections and Treatments
- Spectroscopy and Chemometric Analyses
- Clostridium difficile and Clostridium perfringens research
- Cancer, Stress, Anesthesia, and Immune Response
- Antimicrobial Resistance in Staphylococcus
- Microtubule and mitosis dynamics
- Nanowire Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Force Microscopy Techniques and Applications
- Inflammatory mediators and NSAID effects
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
- Biosensors and Analytical Detection
- Listeria monocytogenes in Food Safety
Czech Academy of Sciences, Institute of Molecular Genetics
2006-2021
University of California, San Diego
2009-2011
University of California, Riverside
2006-2007
Protein Express (United States)
2006
Czech Academy of Sciences
2006
University of Chemistry and Technology, Prague
2000-2001
Staphylococcus aureus is a major cause of community-acquired and nosocomial infections including the life-threatening conditions endocarditis, necrotizing pneumonia, fasciitis, septicemia. Toll-like receptor (TLR)-2, membrane-bound microbial sensor, detects staphylococcal components, but macrophages lacking TLR2 or both TLR4 remain S. responsive, suggesting that an alternative recognition might be involved. The cytoplasmic sensor nucleotide-binding oligomerization domain containing (NOD)...
// Sona Pechackova 1, * , Kamila Burdova Jan Benada 1 Petra Kleiblova 2 Gabriela Jenikova Libor Macurek Department of Cancer Cell Biology, Institute Molecular Genetics the ASCR, CZ-14220 Prague, Czech Republic Biochemistry and Experimental Oncology, Charles University in CZ-12853 These authors have contributed equally to this work Correspondence to: Macurek, e-mail: libor.macurek@img.cas.cz Keywords: WIP1 inhibitor, p53, checkpoint, nutlin-3, breast cancer Received: October 31, 2015...
Galectin-4 and its homologue galectin-6 are members of the tandem-repeat subfamily monomer divalent galectins. Expression mouse galectin-4 by RT-PCR using primers designed to distinguish both galectin transcripts indicates that expressed in small intestine, colon, liver, kidney, spleen heart P19X1 cells while only is BW-5147 3T3 cell lines. In situ hybridization confirmed presence galectin-4/-6 liver intestine. spermatozoons oocytes expression during early emryogenesis appears 8-cell embryos...
A facile technique was demonstrated for the controlled assembly and alignment of multi-segment nanowires using bioengineered polypeptides. An elastin-like-polypeptide (ELP)-based biopolymer consisting a hexahistine cluster at each end (His(6)-ELP-His(6)) generated purified by taking advantage reversible phase transition property ELP. The affinity between His(6) domain biopolymers nickel segment nickel/gold/nickel exploited directed onto peptide-functionalized electrode surfaces. presence...
Abstract Protein phosphatase magnesium-dependent 1 delta (PPM1D) terminates cell response to genotoxic stress by negatively regulating the tumor suppressor p53 and other targets at chromatin. Mutations in exon 6 of PPM1D result production a highly stable, C-terminally truncated PPM1D. These gain-of-function mutations are present various human cancers but their role tumorigenesis remains unresolved. Here we show that impairs activation cycle checkpoints non-transformed RPE cells allows...
The intestinal epithelium is dynamic, with proliferation of undifferentiated crypt cells balanced by terminal differentiation and cell death at the colon surface or small villus tips. Cyclic AMP, induced agonists such as prostaglandin E2 vasoactive polypeptide, promotes ion secretion suppresses apoptosis in epithelial cells. Here, we show that a model leads to attenuation cAMP production response G protein-coupled receptor receptor-independent agonists. Concomitantly, key components cascade,...
In this work, we report a new method to reversibly immobilize proteins surface in functionally active orientation directly from cell lysate by employing fusion protein consisting of thermal-responsive elastin (ELP) domain as the anchor and calcium-responsive calmodulin (CalM) for capturing. Incorporation an M13 tag into recombinant enables not only easy immobilization but also direct purification lysates. The feasibility concept was demonstrated using M13-tagged yellow fluorescent (M13-YFP)....
Abstract Competitive ELISA estimation based on application of polyclonal mouse antibodies to somatic antigen O:9, 12 was developed. The optimization the protocol is reported. optimal concentration immobilized found be 4.9 2 104 cells ml−1; IgG 6.25 w g and peroxidase labelled antibody 8 ml−1. tested exhibited neither cross reactions with chosen strains (serotypes) salmonellas group 04 (B), 07 (C1), 08 (C2-C3), nor members Enterobacteriaceae: Escherichia coli, Klebsiella pneumonia,...
Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which its main means entry into the organism, is a topic special importance. The original classic methods determination are general laborious and time-consuming procedures. In order to address issue we developed new rapid method specific food samples. consists three steps: i) enrichment microflora (16 h), ii) selective isolation sp. exploiting immunomagnetic separation (2–3 h) followed...
Four rapid and simple DNA purification sample treatment protocols were evaluated for detection of Salmonella enterica in spiked minced meat, using a fluorogenic 5’nuclease (TaqMan) PCR assay an ABI‐Prism 7700 Sequence Detector. The limit with the single separation DNeasy was found to be 6–8 CFU just 19 end‐point fluorescence (C t ) values, while this 22 C combination BactXtractor. Extraction by resulted <22, indicating <10 cells per 25g, when samples inoculated before overnight...