A5031702317- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- RNA regulation and disease
- Adenosine and Purinergic Signaling
- Neuroscience of respiration and sleep
- Ion Transport and Channel Regulation
- Hereditary Neurological Disorders
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Metabolism and Genetic Disorders
- Corneal Surgery and Treatments
- COVID-19 and healthcare impacts
- Restless Legs Syndrome Research
- Memory and Neural Mechanisms
- Biochemical Acid Research Studies
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Atherosclerosis and Cardiovascular Diseases
- Neurological diseases and metabolism
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- Traumatic Brain Injury and Neurovascular Disturbances
Dysautonomia Foundation
2023-2025
Arcadia
2024
New York University
2023
University of Rochester
2009-2021
Eastern Virginia Medical School
2020
Thomas Jefferson University Hospital
2020
Propionic Acidemia Foundation
2019
University of Rochester Medical Center
2008-2014
Yale University
2009
Medical Research Council
2008
Forced expression of gap junction proteins, connexins, enables junction-deficient cell lines to propagate intercellular calcium waves. Here, we show that ATP secretion from the poorly coupled lines, C6 glioma, HeLa, and U373 glioblastoma, is potentiated 5- 15-fold by connexin expression. release required purinergic receptor-activated intracellular Ca 2+ mobilization was inhibited Cl − channel blockers. Calcium wave propagation also reduced receptor antagonists blockers but insensitive...
Traumatic spinal cord injury is characterized by an immediate, irreversible loss of tissue at the lesion site, as well a secondary expansion damage over time. Although should, in principle, be preventable, no effective treatment options currently exist for patients with acute (SCI). Excessive release ATP traumatized tissue, followed activation high-affinity P2X7 receptors, has previously been implicated injury, but clinically relevant strategy which to antagonize receptors yet, best our...
Network activity in the brain is associated with a transient increase extracellular K + concentration. The excess removed from space by mechanisms proposed to involve Kir4.1‐mediated spatial buffering, Na /K /2Cl − cotransporter 1 (NKCC1), and/or ‐ATPase activity. Their individual contribution [K ] o management has been of extended controversy. This study aimed, several complementary approaches, delineate transport characteristics Kir4.1, NKCC1, and resolve their involvement clearance...
Glia calcium signaling has recently been identified as a potent modulator of synaptic transmission. We show here that the spatial expansion waves is mediated by ATP and subsequent activation purinergic receptors. Ectopic expression gap junction proteins, connexins (Cxs), leads to an increase in both release radius wave propagation. Cx was also associated with phenotypic transformation, cortical neurons extended longer neurites when co-cultured Cx-expressing than Cx-deficient cells....
Gap junctions are highly conductive channels that allow the direct transfer of intracellular messengers such as Ca 2+ and inositol triphosphate (IP 3 ) between interconnected cells. In brain, astrocytes coupled extensively by gap junctions. We found here among in acutely prepared brain slices well culture remained open during ischemic conditions. Uncoupling first occurred after terminal loss plasma membrane integrity. therefore may link an evolving infarct with surroundings. The free...
A hallmark of astrocytic tumors is their infiltrative nature. Although aggressive and typically widespread dispersal in the adult brain differs fundamentally from that other tumors, little known about cellular basis. Astrocytic express gap junction protein connexin 43 (Cx43), we show here Cx43 expression induced morphological transformation glioma cells into an epithelial phenotype. In a short-term aggregation assay, was associated with several-fold increase competence to aggregate....
We have studied the role of actin fiber assembly on calcium signaling in astrocytes. found that (1) after astrocytes been placed culture, it takes several hours for organization definitive cytoskeleton. Actin and number cells engaged increased parallel. (2) Disruption cytoskeleton attenuated wave propagation; cytochalasin D treatment reduced signaling. (3) Propagation waves depends cytoskeletal function; inhibition myosin light chain kinase suppressed activity. (4) Astrocytic is mediated by...
Abstract Gap junctions are intercellular channels formed by hemichannels (or connexons) from two neighboring cells. Hemichannels, which composed of proteins called connexins, can function as conduits ATP and glutamate, interact with adhesion molecules other signaling elements. As a result, their functional repertoire is expanding into roles, such control cell growth or migration. Here we further elucidate the involvement in cell–cell analyzing how connexins regulate without need gap junction...
In this study, we investigated whether the ability of Eph receptor signaling to mediate cell repulsion is antagonized by fibroblast growth factor (FGFR) activation that can promote invasion. We find FGFR1 in EphB2-expressing cells prevents segregation, repulsion, and collapse responses ephrinB1 ligand. leads increased phosphorylation unstimulated EphB2, which show caused down-regulation leukocyte common antigen-related tyrosine phosphatase dephosphorylates EphB2. addition, inhibits further...
Glucocorticoids are the first line of choice in treatment cerebral edema associated with brain tumors. High-dose glucocorticoids reduce extent within hours, often relieving critical increases intracranial pressure, but mechanisms by which modulate water content not well-understood. A possible target action may be glucocorticoid receptor-expressing astrocytes, primary regulators interstitial ion homeostasis brain. In this study, we demonstrate that two glucocorticoids, methylprednisolone and...
Mechanical stimulation of adult human and rat pia-arachnoid cell cultures (loaded with calcium indicator dye) produced an increase in the stimulated cell. This change then propagated rapidly among neighboring cells, producing a wave maximum distance propagation velocity resembling waves astrocytes. The were blocked by either octanol or apyrase, suggesting that might occur gap junction communication extracellular movement ATP. Calcium cells could invade contiguous astrocytes, vice versa. Gap...
Abstract Propionic Acidemia (PA) is characterized by the accumulation of propionic acid (PPA), its toxic derivatives, and ammonia. The disease causes multiorgan damage, especially in heart, pancreas, brain; seizures intellectual disability are often described. Some PA children also show autism spectrum disorders (ASD). In this study, we have compiled data from 62 individuals International Patient Registry compared it to published literature on prevalence PA. registry shows a significant...