A5015604534- 14-3-3 protein interactions
- Circadian rhythm and melatonin
- Tryptophan and brain disorders
- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Radiopharmaceutical Chemistry and Applications
- Photosynthetic Processes and Mechanisms
- Complement system in diseases
- Medical Imaging and Pathology Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Orthopedic Infections and Treatments
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Lipid metabolism and biosynthesis
- Viral Infections and Vectors
- Light effects on plants
- COVID-19 Clinical Research Studies
- Histone Deacetylase Inhibitors Research
- Plant Reproductive Biology
- Treatment of Major Depression
- Neuroscience and Neuropharmacology Research
- Protein Tyrosine Phosphatases
Jamia Hamdard
2017-2024
Center for Interdisciplinary Studies
2023
Institute of Molecular Medicine
2010-2020
Variable Energy Cyclotron Centre
2010
National Institutes of Health
2001-2008
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2001-2008
Scripps Research Institute
2007
Johns Hopkins University
2006-2007
Johns Hopkins Medicine
2006
Physiologie de la Reproduction et des Comportements
2005
The nocturnal increase in circulating melatonin vertebrates is regulated by the activity of arylalkylamine N -acetyltransferase (AANAT), penultimate enzyme pathway (serotonin → -acetylserotonin melatonin). Large changes are linked to cyclic AMP-dependent protein kinase-mediated phosphorylation AANAT T31. Phosphorylation T31 promotes binding dimeric 14-3-3 protein, which activates increasing affinity. In current study, a putative second kinase site, S205, was found be ≈55% phosphorylated at...
The daily rhythm in melatonin levels is controlled by cAMP through actions on the penultimate enzyme synthesis, arylalkylamine N -acetyltransferase (AANAT; serotonin -acetyltransferase, EC 2.3.1.87 ). Results presented here describe a regulatory/binding sequence AANAT that encodes cAMP-operated binding switch which cAMP-regulated protein kinase-catalyzed phosphorylation [RRHTLPAN → RRHpTLPAN] promotes formation of complex with 14-3-3 proteins. Formation this AANAT/14-3-3 enhances production...
The pineal gland plays an essential role in vertebrate chronobiology by converting time into a hormonal signal, melatonin, which is always elevated at night. Here we have analyzed the rodent transcriptome using Affymetrix GeneChip® technology to obtain more complete description of cell biology. effort revealed that 604 genes (1,268 probe sets) with Entrez Gene identifiers are differentially expressed greater than 2-fold between midnight and mid-day (false discovery rate <0.20). Expression...
Negative-strand RNA viruses form cytoplasmic inclusion bodies (IBs) representing virus replication foci through phase separation or biomolecular condensation of viral and cellular proteins, as a hallmark their infection. Alternatively, mammalian cells stalled mRNA containing antiviral stress granules (SGs), consequence phosphorylation eukaryotic initiation factor 2α (eIF2α) several RNA-binding proteins including TIA-1. Whether how Chandipura (CHPV), an emerging human pathogen causing...
Interleukin-12 (IL-12) demonstrates potent antitumor activity by enhancing Th1/Th2 response, facilitating cytotoxic T-cell (CTL) recruitment into tumors, inhibiting tumor angiogenesis, and depleting immunosuppressive cells in the microenvironment (TME). Despite having encouraging preclinical some clinical results, further development of IL-12 is limited because dose-limiting toxicity observed early trials with systemic administration recombinant human IL-12. Hence, strategies aiming to lower...
The E-26 transforming specific (ETS)-related gene TEL , also known as ETV6 is involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. encoded protein contains two functional domains: helix–loop–helix (HLH) domain (also pointed domain) located at the N terminus DNA-binding C terminus. HLH protein–protein interaction itself other members ETS family transcription factors such FLI1. factor, we others have shown that it repressor expression....
Abstract Otx2 is a vertebrate homeobox gene, which has been found to be essential for the development of rostral brain regions and appears play role in retinal photoreceptor cells pinealocytes. In this study, temporal expression pattern was revealed rat brain, with special emphasis on pineal gland throughout late embryonic postnatal stages. Widespread high becomes progressively restricted adult gland. Crx ( cone–rod ), downstream target gene Otx2, showed similar that , although there...
Arylalkylamine <i>N</i>-acetyltransferase (AANAT, serotonin <i>N-</i>acetyltransferase, EC 2.3.1.87) plays a unique transduction role in vertebrate physiology by converting information about day and night into hormonal signal: melatonin. Only members of the AANAT family have been functionally characterized. Here putative from <i>Saccharomyces cerevisiae</i> (scAANAT) was studied to determine whether it possessed catalytic activity enzyme. scAANAT is 47% similar ovine AANAT, but lacks...
The aim of this study was to radiolabel ciprofloxacin (Cip) and nitrofuryl thiosemicarbazone (NFT) with the fac-[99mTc(CO)3(H2O)3]+ core evaluate ability radiopharmaceuticals as tracers in detecting sites infection. Cip NFT were radiolabeled characterized by RHPLC. stabilities preparations evaluated saline rat serum. In vitro binding studies S. aureus performed. Biodistribution conducted at different time points after injecting (i.v.) rats (intramuscularly infected aureus) well sterile...
Serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) is a member of the GCN5 (GNAT) superfamily and catalyzes penultimate step in biosynthesis melatonin; large daily rhythm AANAT activity drives circulating melatonin. We have used structure-based computational approach to identify first druglike selective inhibitors AANAT. Approximately 1.2 million compounds were virtually screened by 3D high-throughput docking into active site X-ray structures for AANAT, total 241...
Arylalkylamine N-acetyltransferase (AANAT) catalyzes the N-acetylation of serotonin, penultimate step in synthesis melatonin. Pineal AANAT activity increases at night all vertebrates, resulting increased melatonin production. This circulating levels melatonin, thereby providing a hormonal signal darkness. Kinetic and structural analysis has determined that one element is floppy. element, termed Loop 1, three loops comprise arylalkylamine binding pocket. During course chordate evolution, 1...
The present study was done for characterization and antibiotic sensitivity pattern of theSalmonella spp. found as contaminants in chicken carcass. overall incidence ofSalmonella contamination poultry carcass to be 4.90% with the higher percentage of Salmonella being isolated from meat (8.00%) followed by liver spleen (6.25%). isolates were identified Salmonella Typhimurium, SalmonellaParatyphi B and Salmonella (Rough). Eight Salmonella isolates obtained confirmed as Salmonella spp. according...