Abstract Sirtuin 6 (SIRT6) is a NAD-dependent deacetylase involved in lifespan regulation. To evaluate the effect of SIRT6 on osteogenesis, rat bone marrow mesenchymal stem cells (rBMSCs) with enhanced or reduced function were developed. We observed that knockdown significantly mRNA levels several key osteogenic markers vitro, including alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and osteocalcin, while overexpression their expression. Additionally, activated...

Directly reprogramming fibroblasts into cardiomyocytes improves cardiac function in the infarcted heart. However, low efficacy of this approach hinders clinical applications. Unlike adult mammalian heart, neonatal heart has an intrinsic regenerative capacity. Consequently, we hypothesized that birth imposes fundamental changes which limit their capabilities. In support, found vitro was markedly lower with derived from mice versus those mice. Notably, expressed significantly higher levels...

The aim of this study was to investigate the effect silane pretreatment on universal adhesive bonding between lithium disilicate glass ceramic and composite resin. IPS e.max blocks etched with hydrofluoric acid were randomly assigned one eight groups treated four adhesives (two silane‐free two silane‐containing adhesives), each or without pretreatment. Bonded specimens stored in water for 24 h. shear bond strength (SBS) ceramic‐resin interface measured evaluate strength, debonded after SBS...

Yes-associated protein 1 (YAP1), the core downstream effector of Hippo signaling cascade, was involved in regulation osteoblast and osteoclast differentiation bone metabolism. However, regulatory effects mechanisms YAP1 on bone-remodeling molecules osteoblasts under inflammation remain unknown. In this study, expression level downregulated after treatment with inflammatory cytokine tumor necrosis factor-α (TNF-α) MC3T3-E1 cells. The key osteoclastogenic induced by TNF-α, namely,...

Stem cell injections are an attractive therapeutic tool. It has been demonstrated that injected stem cells promote tissue repair and regeneration via paracrine mechanisms. However, the effects of continue for far longer than they present. We hypothesized prolonged because a sequential relay mechanism. Conditioned media was collected from mesenchymal (MSCs) after 24 h. This then added to RAW264.7. Media macrophages h endothelial (ECs). conditioned macrophage media, but not control promoted...

Abstract Tooth cementum is a bone‐like mineralized tissue and serves as microbial barrier against invasion destruction. Cementum also responsible for tooth stability defending pulp from outside stimuli, which formed by cementoblasts. Although it crucial periodontal periapical diseases, the mechanisms underlying pathophysiological changes of cementoblasts their inflammatory responses remain unclear. MiR‐181b found to modulate vascular inflammation endotoxin tolerance. In this study,...

Abstract Iroquois homeobox gene 5 ( Irx5 ) is a highly conserved member of the family. Members this family play distinct and overlapping roles in normal embryonic cell patterning development malignancies. In study, we observed that IRX was abnormally abundant tongue squamous carcinoma TSCC tissues lines. We used gain‐ loss‐of‐function methods to overexpress knockdown expression line CAL 27. Our results elucidated elevated levels promoted proliferation, migration invasion cells, whereas...

Cerebral microbleeds (CMBs) have been observed in the elderly and regarded as a manifestation of small vessel disease (SVD). glomerular SVD may common source pathogenesis because these organs are closely connected through anatomic hemodynamic similarities. The purpose this study was to clarify associations between kidney biomarker levels CMBs hypertensive patients.The presence number were assessed on susceptibility-weighted imaging. We calculated urinary albumin/creatinine ratio (UACR) from...

Yes‐associated protein 1 (YAP1) transcriptional coactivator is a mediator of mechanosensitive signaling. Cementum, which covers the tooth root surface, continuously senses external mechanical stimulation. Cementoblasts are responsible for mineralization and maturation cementum. However, effect YAP1 on cementoblast differentiation remains largely unknown. In this study, we initially demonstrated that overexpression enhanced ability cementoblasts. upregulated mRNA expression several...

Background and purpose The association between the presence of cerebral microbleeds (CMBs) serum vascular endothelial growth factor (VEGF) levels in patients with Alzheimer's disease (AD) was investigated. Methods Consecutive AD who underwent magnetic resonance examination susceptibility‐weighted imaging (SWI) were prospectively examined. CMBs on SWI independently interpreted. number location assessed. Demographics including age, sex risk factors obtained. Serum VEGF measured an...

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Abstract Human dental pulp cells ( HDPC s) play a crucial role in inflammation. Pannexin 3 (Panx3), member of Panxs (Pannexins), has been recently found to be involved However, the mechanism Panx3 human inflammation remains unclear. In this study, inflammatory response was firstly explored, and its potential proposed. Immunohistochemical staining showed that levels were diminished inflamed rat tissues. vitro , expression significantly down‐regulated s following TNF ‐α challenge...

Abstract Yes‐associated protein 1 (YAP1) transcriptional coactivator has recently been identified to regulate skeletal lineage cell differentiation and bone development. However, the role molecular mechanisms of YAP1 in regulation osteoblastic remains be elucidated. In this study, we demonstrated that expression was increased during osteogenic rat mesenchymal stem cells MC3T3‐E1. overexpression MC3T3‐E1 showed osteogenesis markers, such as runt‐related transcription factor 2, osteocalcin,...

Background/Aims: Human dental pulp-derived mesenchymal stromal cells (hDPSCs) are promising seed for tissue engineering due to their easy accessibility and multi-lineage differentiation. Pannexin3 (Panx3) plays crucial roles during bone development The aim of the present study was investigate effect Panx3 on osteogenesis hDPSCs underlying mechanism. Methods: Utilizing qRT-PCR, Western blot, immunohistochemistry, we explored change osteogenic differentiation hDPSCs. Next, with loss (Panx3...

Abstract Cementum, which shares common features with bone in terms of biochemical composition, is important for the homeostasis periodontium during periodontitis and orthodontic treatment. Sirtuin 6 (SIRT6), as a member sirtuin family, plays key roles osteogenic differentiation marrow mesenchymal stem cells. However, involvement SIRT6 cementoblast mineralization underlying mechanisms remain unknown. In this study, we observed that expression increased initially. Analysis gain‐...

Intracellular thiols play vital roles in living systems, and their situ monitoring is of great importance. Here, we report on a bioorthogonal chemistry based fluorescent probe, which capable intracellular cells for up to 36 hours with an obvious blue-to-green fluorescence change.

DDIT3 is of great importance in endoplasmic reticulum stress and involved many inflammatory diseases mineralization processes. The cementum protects teeth from periodontitis provides attachment for Sharpey's fibers the periodontal ligament. However, effect on cementoblast differentiation remains largely unknown. In this study, we found that was suppressed during differentiation. Knockdown increased messenger RNA (mRNA) protein levels several key osteogenic markers vitro, including alkaline...

The regeneration of craniofacial bone defects remains a crucial clinical challenge. To date, numerous biomaterials are applied in this field. However, current strategies have ignored the importance intramembranous ossification and vital role macrophages regulating osteogenesis. Here, an osteoblast (OB)-targeting peptide (SDSSD)-modified chitosan scaffold (CS-SDSSD) is developed for imitating physiological process development from fibrous membrane. addition free (fSDSSD) can recruit host OBs,...