A5082366017- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related Molecular Pathways
- Neuropeptides and Animal Physiology
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Anesthesia and Neurotoxicity Research
- Cancer Research and Treatments
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Alzheimer's disease research and treatments
- Blood properties and coagulation
- Cancer therapeutics and mechanisms
- Acute Myeloid Leukemia Research
- Neuroendocrine Tumor Research Advances
- Cancer-related gene regulation
- Science, Research, and Medicine
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Infant Nutrition and Health
Inserm
2003-2024
Institut Gustave Roussy
2014-2024
Laboratoire de Biologie et Pharmacologie Appliquée
1989-2017
Centre National de la Recherche Scientifique
2002-2013
École Normale Supérieure Paris-Saclay
2010-2013
École Normale Supérieure - PSL
2009
Molecular Engines Laboratories (France)
2001-2008
Institut Curie
2004
Université Toulouse III - Paul Sabatier
2000-2004
Laboratoire de Microbiologie et Génétique Moléculaires
2004
Tumor reversion is the process by which some cancer cells lose their malignant phenotype. This study was aimed at defining of molecular and phenotypic properties this process. Biological models tumor were isolated from human leukemia breast cell lines using H-1 parvovirus as a selective agent. Differential gene expression analysis performed between parental revertants or alternatively these SIAH-1 transfectant counterparts. These transfectants have suppressed phenotype used control for...
By analyzing the gene expression profile between tumor cells and revertant counterparts that have a suppressed malignant phenotype, we previously reported significant down-regulation of translationally controlled protein (TCTP) in revertants. In present study, derived, by using H1 parvovirus as selective agent, revertants from three major solid cancers: colon, lung, melanoma cell lines. These strongly phenotype both vitro vivo. The level TCTP is decreased most To verify whether inhibition...
We measured the human pim-1 protooncogene (PIM) expression during fetal development and in hematopoietic malignancies. Our data indicate that hematopoiesis 33-kDa pim product, p33pim, is highly expressed liver spleen. In contrast, at adult stage it only slightly circulating granulocytes. Out of 70 malignancies analyzed, 51 patients 19 cell lines, p33pim was overexpressed approximately 30% samples, particularly myeloid lymphoid acute leukemias. This overexpression unrelated to any cellular...
Translationally controlled tumor protein (TCTP) is cytoplasmic and structurally related to guanine-nucleotide free chaperones. TCTP (also called histamine-releasing factor) has been described previously as a secreted that participates in inflammatory responses by promoting the release of histamine. How eventually exported out cell promote such activities unknown. Here we show secretion was insensitive either brefeldin A or monensin, suggesting it proceeds via an endoplasmic...
Recently, we demonstrated that the expression levels of translationally controlled tumor protein (TCTP) were strongly down-regulated at mRNA and during reversion/suppression by activation p53 Siah-1. To better characterize function TCTP, a yeast two-hybrid hunt was performed. Subsequent analysis identified translation elongation factor, eEF1A, its guanine nucleotide exchange eEF1Bbeta, as TCTP-interacting partners. In vitro in vivo studies confirmed TCTP bound specifically eEF1Bbeta eEF1A....
We report the isolation of 10 differentially expressed cDNAs in process apoptosis induced by p53 tamor suppressor. As a global analytical method, we performed differential display mRNA between mouse M1 myeloid leukemia cells and derived clone LTR6 cells, which contain stably transfected temperature-sensitive mutant p53. At 32 degrees C wild-type function is activated resulting programmed cell death. Eight genes are (TSAP; tumor suppressor pathway), two inhibited (TSIP, pathway) their...
Presenilins 1 and 2 are two homologous proteins that, when mutated, account for most early onset Alzheimer's disease. Several lines of evidence suggest among various functions, presenilins could modulate cell apoptotic responses. Here we establish that the overexpression presenilin (PS2) its mutated form Asn-141-Ile-PS2 alters viability human embryonic kidney (HEK)293 cells as established by combined trypan blue exclusion, sodium...
The p53 tumor suppressor protein plays a crucial role in tumorigenesis by controlling cell-cycle progression and apoptosis. We have previously described transcript designated activated pathway-6 (TSAP6) that is up-regulated the p53-inducible cell line, LTR6. Cloning of murine human full-length TSAP6 cDNA revealed it encodes 488-aa with five to six transmembrane domains. This gene homologue recently published rat pHyde. Antibodies raised against recognize 50- 55-kDa band induced p53. Analysis...
We have previously described biological model systems for studying tumor suppression in which, by using H-1 parvovirus as a selective agent, cells with strongly suppressed malignant phenotype (KS or US) were derived from cell lines (K562 U937). By cDNA display on the K562/KS cells, 15 cDNAs now isolated, corresponding to genes differentially regulated suppression. Of these, TSAP9 corresponds TCP-1 chaperonin, TSAP13 regulatory proteasome subunit, and TSAP21 syntaxin 11, vesicular trafficking...
Developmentally regulated genes in Drosophila, which are conserved through evolution, potential candidates for key functions biological processes such as cell cycle, programmed death, and cancer. We report cloning characterization of the human homologue Drosophila seven absentia gene (HUMSIAH), codes a 282 amino acids putative zinc finger protein. HUMSIAH is localized on chromosome 16q12-q13. This activated during physiological program death intestinal epithelium. Moreover, cancer-derived...
The Drosophila Seven in absentia ( Sina ) gene product originally was described as a protein that controls cell fate decisions during eye development. Its mammalian homolog, Siah-1, recently found to be involved p53-dependent and -independent pathways of apoptosis G 1 arrest. We report Siah-1 interacts directly with promotes the degradation regulator Numb. Siah-1-mediated Numb leads redistribution endogenous cell-surface Notch cytoplasm nucleus augmented Notch-regulated transcriptional...
A model system is proposed to investigate, at the molecular level, pathways of tumor suppression. As a tool for selection cells with suppressed phenotype, we used H-1 parvovirus that preferentially kills various neoplastic cells. From human K562 leukemia cells, isolated clone, KS, resistant cytopathic effect virus and displays malignant phenotype. The malignancy cellular resistance killing appear depend on activity wild-type p53. Whereas KS express p53, protein undetectable in parental...
Siah proteins are E3 ubiquitin ligases. They homologues of the Drosophila seven in absentia (Sina), a protein required for R7 photoreceptor development. We have previously found that expression human siah-1 and its mouse homologue siah-1b induced by p53 during apoptosis tumor reversion. So far, no evidence gene is direct transcriptional target has been provided. In present study we investigate this issue. Northern blot analysis with specific probe demonstrates an increase transcription on...
Abstract Monoclonal antibodies specific to human T lymphocyte receptors are currently being used define the biochemical structure of these proteins as well functionally distinct cell subsets. Since one (OKT3) recognizing T3 (CD3) receptor mimics vital physiological processes involved in activation immune system and has been successfully a therapeutical agent, we investigated mechanisms underlying this antibody‐receptor interaction. Our results show that after binding OKT3, complex (OKT3‐T3)...
Abstract Translationally Controlled Tumor Protein (TCTP) is anti-apoptotic, key in development and cancer, however without the typical Bcl2 family members’ structure. Here we report that TCTP contains a BH3-like domain forms heterocomplexes with Bcl-xL. The crystal structure of Bcl-xL deletion variant-TCTP 11–31 complex reveals refolds helical conformation upon binding BH3-groove Bcl-xL, although lacking h1 -subregion interaction. Experiments using vitro-vivo reconstituted systems +/− mice...