A5079972049- Ion Channels and Receptors
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Ion channel regulation and function
- Connexins and lens biology
- Retinal Imaging and Analysis
- Magnesium in Health and Disease
- Retinoids in leukemia and cellular processes
- Neurobiology and Insect Physiology Research
- Neuropeptides and Animal Physiology
- Cardiovascular Issues in Pregnancy
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Growth Hormone and Insulin-like Growth Factors
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Retinopathy of Prematurity Studies
- Circadian rhythm and melatonin
- Glaucoma and retinal disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuroscience and Neural Engineering
- Phytochemicals and Antioxidant Activities
- Eicosanoids and Hypertension Pharmacology
- Biochemical Analysis and Sensing Techniques
Universidad Nacional Autónoma de México
2013-2024
Institut de Biologie Intégrative de la Cellule
2024
Université Paris-Saclay
2024
Centre National de la Recherche Scientifique
2010-2024
CEA Paris-Saclay
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2024
Instituto de Neurologia Y Neurocirugia
2022
Inserm
2003-2016
Université de Strasbourg
2016
Institut de génétique et de biologie moléculaire et cellulaire
2016
Primary hypomagnesemia constitutes a rare heterogeneous group of disorders characterized by renal or intestinal magnesium (Mg2+) wasting resulting in generally shared symptoms Mg2+ depletion, such as tetany and generalized convulsions, often including associated disturbances calcium excretion. However, most the genes involved physiology handling are unknown. Through discovery mutation EGF gene isolated autosomal recessive hypomagnesemia, we have, for what believe is first time, identified...
Abstract One major clinical problem with prostate cancer is the cells' ability to survive and proliferate upon androgen withdrawal. Because Ca2+ central growth control, understanding mechanisms of homeostasis involved in cell proliferation imperative for new therapeutic strategies. Here, we show that agonist-mediated stimulation α1-adrenergic receptors (α1-AR) promotes primary human epithelial (hPCE) cells by inducing store-independent entry subsequent activation nuclear factor activated T...
In recent years, the transient receptor potential melastatin member 8 (TRPM8) channel has emerged as a promising prognostic marker and putative therapeutic target in prostate cancer (PCa). However, mechanisms of prostate-specific regulation functional evolution TRPM8 during PCa progression remain unclear. Here we show, for first time to our knowledge, that only secretory mature differentiated human primary epithelial (PrPE) luminal cells expressed plasma membrane ((PM)TRPM8) channels....
Recent identification of a mutation in the EGF gene that causes isolated recessive hypomagnesemia led to finding increases activity epithelial magnesium (Mg2+) channel transient receptor potential M6 (TRPM6). To investigate molecular mechanism mediating this effect, we performed whole-cell patch-clamp recordings TRPM6 expressed human embryonic kidney 293 (HEK293) cells. Stimulation increased current through but not TRPM7. The carboxy-terminal alpha-kinase domain did participate...
Recent cloning of a cold/menthol-sensitive TRPM8 channel (transient receptor potential melastatine family member 8) from rodent sensory neurons has provided the molecular basis for cold sensation. Surprisingly, human orthologue also appears to be strongly expressed in prostate and cancer-derived epithelial cell line, LNCaP. In this study, we show that despite such expression, LNCaP cells respond cold/menthol stimulus by membrane current (Icold/menthol) shows inward rectification high Ca2+...
TRPM8 represents an ion channel activated by cold temperatures and cooling agents, such as menthol, that underlies the cold-induced excitation of sensory neurons. Interestingly, only human tissue outside peripheral nervous system, in which expression transcripts has been detected at high levels, is prostate, a not exposed to any essential temperature variations. Here we show cloned from prostate heterologously expressed HEK-293 cells regulated Ca(2+)-independent phospholipase A(2) (iPLA(2))...
Chondrocytes are the only cells in cartilage, and their death by apoptosis contributes to cartilage loss inflammatory joint diseases, such as rheumatoid arthritis (RA). A putative therapeutic intervention for RA is inhibition of apoptosis-mediated degradation. The hormone prolactin (PRL) frequently increases circulation patients with RA, but role hyperprolactinemia disease activity unclear. Here, we demonstrate that PRL inhibits cultured chondrocytes response a mixture proinflammatory...
Prostate smooth muscle cells predominantly express alpha1-adrenoceptors (alpha1-AR). alpha1-AR antagonists induce prostate relaxation and therefore they are useful therapeutic compounds for the treatment of benign prostatic hyperplasia symptoms. However, Ca(2+) entry pathways associated with activation in have yet to be elucidated. In many cell types, mammalian homologues transient receptor potential (TRP) genes, first identified Drosophila, encode TRPC (canonical TRP) proteins. They...
The one or more coupling mechanisms of store-operated channels (SOCs) to endoplasmic reticulum (ER) Ca2+ store depletion as well the molecular identity SOCs per se still remain a mystery. Here, we demonstrate co-existence two populations distinct endogenous in LNCaP prostate cancer epithelial cells, which are preferentially activated by either active inositol 1,4,5-trisphosphate (IP3)-mediated passive thapsigargin-facilitated and have different ER content sensitivity. first population,...
Increased retinal vasopermeability contributes to diabetic retinopathy, the leading cause of blindness in working-age adults. Despite clinical progress, effective therapy remains a major need. Vasoinhibins, family peptides derived from protein hormone prolactin (and inclusive 16-kDa fragment prolactin), antagonize proangiogenic effects VEGF, primary mediator vasopermeability. Here, we demonstrate what believe be novel function vasoinhibins as inhibitors increased associated with retinopathy....
OBJECTIVE Increased retinal vasopermeability (RVP) occurs early in diabetes and is crucial for the development of sight-threatening proliferative diabetic retinopathy (DR). The hormone prolactin (PRL) proteolytically processed to vasoinhibins, a family peptides that inhibit excessive RVP related DR. Here, we investigate circulating levels PRL association with DR men test whether increased PRL, by serving as source ocular can reduce pathological diabetes. RESEARCH DESIGN AND METHODS Serum was...
The identification of pathways necessary for retinal pigment epithelium (RPE) function is fundamental to uncover therapies blindness. Prolactin (PRL) receptors are expressed in the retina, but nothing known about role PRL RPE. Using adult RPE 19 (ARPE-19) human cell line and mouse RPE, we identified presence demonstrated that survival via anti-apoptotic antioxidant actions. promotes capacity ARPE-19 cells by reducing glutathione. It also blocks hydrogen peroxide-induced increase deacetylase...
Retinal degeneration is characterized by the progressive destruction of retinal cells, causing deterioration and eventual loss vision. We explored whether hormone prolactin provides trophic support to thus protecting retina from degenerative pressure. Inducing hyperprolactinemia limited photoreceptor apoptosis, gliosis, changes in neurotrophin expression, it preserved photoresponse phototoxicity model degeneration, which continuous exposure rats bright light leads cell death dysfunction. In...
Abstract Translationally Controlled Tumor Protein (TCTP) is anti-apoptotic, key in development and cancer, however without the typical Bcl2 family members’ structure. Here we report that TCTP contains a BH3-like domain forms heterocomplexes with Bcl-xL. The crystal structure of Bcl-xL deletion variant-TCTP 11–31 complex reveals refolds helical conformation upon binding BH3-groove Bcl-xL, although lacking h1 -subregion interaction. Experiments using vitro-vivo reconstituted systems +/− mice...
The prostate gland is a rich source of α1-adrenergic receptors (α1-ARs). α1-AR antagonists are commonly used in the treatment benign prostatic hyperplasia symptoms, due to their action on smooth muscle cells. However, virtually nothing known about role α1-ARs epithelial Here, by using two human cancer (hPCE) cell models — primary cells from resection specimens (primary hPCE cells) and an LNCaP (lymph node carcinoma prostate) line we identify α1A subtype adrenergic receptor (α1A-AR) show its...
Prolactin (PRL) stimulates the secretion of oxytocin (OXT) and arginine AVP as part maternal adaptations facilitating parturition lactation. Both neurohormones are under regulation nitric oxide. Here, we investigate whether activation neuronal oxide synthase (nNOS) in hypothalamo-neurohypophyseal system mediates effect PRL on OXT release these effects operate males. Plasma levels were measured male rats after intracerebroventricular injection or inducing hyperprolactinemia by placing two...