A5069646620- Health, Medicine and Society
- Ion channel regulation and function
- Ion Channels and Receptors
- Nicotinic Acetylcholine Receptors Study
- Neurobiology and Insect Physiology Research
- Pancreatic function and diabetes
- Growth Hormone and Insulin-like Growth Factors
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Protein Hydrolysis and Bioactive Peptides
- Hypothalamic control of reproductive hormones
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Nitric Oxide and Endothelin Effects
- Photoreceptor and optogenetics research
- Graphene and Nanomaterials Applications
- Mass Spectrometry Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Bioactive Compounds and Antitumor Agents
- Diet, Metabolism, and Disease
- Heat shock proteins research
Laboratoire de Physiologie Cellulaire
2008-2024
Université de Lille
2003-2024
Inserm
2008-2024
La Ligue Contre le Cancer
2009-2024
Centre National de la Recherche Scientifique
1991-2024
Université Polytechnique Hauts-de-France
2024
École Centrale de Lille
2024
Institut d'électronique de microélectronique et de nanotechnologie
2024
Onco Lille
2020
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2012-2017
Abstract One major clinical problem with prostate cancer is the cells' ability to survive and proliferate upon androgen withdrawal. Because Ca2+ central growth control, understanding mechanisms of homeostasis involved in cell proliferation imperative for new therapeutic strategies. Here, we show that agonist-mediated stimulation α1-adrenergic receptors (α1-AR) promotes primary human epithelial (hPCE) cells by inducing store-independent entry subsequent activation nuclear factor activated T...
Defects in the human protein TMEM165 are known to cause a subtype of Congenital Disorders Glycosylation. Transmembrane 165 (TMEM165) belongs an uncharacterized family membrane proteins called Uncharacterized Protein Family 0016, which well conserved throughout evolution and share characteristics reminiscent cation/Ca 2+ exchanger superfamily. Gcr1 dependent translation factor 1 (Gdt1p), budding yeast member this family, contributes Ca homeostasis via transport pathway localized Golgi...
Neuroendocrine differentiation of prostate epithelial cells is usually associated with an increased aggressivity and invasiveness tumors a poor prognosis. However, the molecular mechanisms involved in this process remain poorly understood. We have investigated possible expression voltage-gated calcium channels human cancer LNCaP their modulation during neuroendocrine differentiation. A small proportion undifferentiated displayed voltage-dependent current. This current density were...
Bisphenol A (BPA), the principal constituent of reusable water bottles, metal cans, and plastic food containers, has been shown to be involved in human prostate cancer (PCa) cell proliferation. The aim present study was explore effect BPA on PCa migration pathways these processes. Using transwell technique, we clearly show for first time that pre-treatment cells with (1-10 nM) induces migration. a calcium imaging an amplification Store-Operated Calcium Entry (SOCE) LNCaP cells. RT-PCR...
Gold nanorods (Au NRs) are known for their efficient conversion of photon energy into heat, resulting in hyperthermia and suppression tumor growths <italic>in vitro</italic> vivo</italic>.
1. In the present study, we investigated mechanisms involved in induction of apoptosis by Ca2+-ATPase inhibitor thapsigargin (TG), androgen-sensitive human prostate cancer LNCaP cells. 2. Exposure fura-2-loaded cells to TG presence extracellular calcium produced an increase intracellular Ca2+, first phase which was associated with depletion stores and second one consecutive Ca2+ entry through plasma membrane, store-operated channels (SOCs). 3. For time have identified characterized...
Because prostate cancer is, in its early stages, an androgen-dependent pathology, treatments aiming at decreasing testosterone plasma concentration have been developed for many years now. However, a significant proportion of patients suffer relapse after few hormone therapy. The androgen-independent stage has shown to be associated with the development neuroendocrine differentiation. We previously demonstrated that cells derived from LNCaP overexpress CaV3.2 T-type voltage-dependent calcium...
Previous studies showed the effects of resveratrol (RES) on several cancer cells, including prostate (PCa) cell apoptosis without taking into consideration impact tumor microenvironment (TME). The TME is composed endothelial blood and cancer‐associated fibroblasts (CAF), main source growth factors. latter cells might modify in RES via secreted Recent data clearly show CAF resistance However, PCa have not been studied so far. We investigated here for first time physiology context TME. Using a...
It is strongly suspected that potassium (K(+)) channels are involved in various aspects of prostate cancer development, such as cell growth. However, the molecular nature those K(+) implicated proliferation and mechanisms through which they control still unknown. This study uses pharmacological, biophysical approaches to show main voltage-dependent current LNCaP cells carried by large-conductance BK channels. Indeed, most was inhibited inhibitors (paxillin iberiotoxin) siRNA targeting In...
Accruing evidence indicates that exposure to environmental compounds may adversely affect human health and promote carcinogenesis. Triclosan (TCS), an antimicrobial agent widely used as a preservative in personal care products, has been shown act endocrine disruptor hormone-dependent tissues. Here, we demonstrate new molecular mechanism by which TCS stimulates the secretion prostate cancer stromal cells of vascular endothelial growth factor (VEGF), known tumor growth. This involves increase...
Sodium (Na+) ions are known to regulate many signaling pathways involved in both physiological and pathological conditions. In particular, alterations intracellular concentrations of Na+ corresponding changes membrane potential be major actors cancer progression metastatic phenotype. Though the functionality channels currents can investigated using patch-clamp technique, latter is rather invasive a technically difficult method study transients compared fluorescence imaging. Despite fact that...
Abstract There is growing evidence that ion channels are critically involved in cancer cell invasiveness and metastasis. However, the molecular mechanisms of signaling promoting behavior poorly understood complexity underlying remodeling during metastasis remains to be explored. Here, using a variety vitro vivo techniques, we show metastatic prostate cells acquire specific Na + /Ca 2+ signature required for persistent invasion. We identify leak channel, NALCN, which overexpressed cancer, as...
The present study demonstrates for the first time that intracellular calcium-ATPases and calcium pool content are closely associated with prostate cancer LNCaP cell growth. Cell growth was modulated by changing amount of epidermal factor, serum, androgene in culture media. Using microspectrofluorimetric method Fura-2 Mag as probes, we show these cells, rate is correlated content. Indeed, an increased increase filling state, whereas growth-inhibited cells a reduced load. Western blotting...
// Gabriel Bidaux 1, 2, 3, * , Anne-Sophie Borowiec Charlotte Dubois 2 Philippe Delcourt Céline Schulz Fabien Vanden Abeele Gilbert Lepage Emilie Desruelles Alexandre Bokhobza Etienne Dewailly Christian Slomianny Morad Roudbaraki Laurent Héliot 3 Jean-Louis Bonnal Brigitte Mauroy Pascal Mariot Loïc Lemonnier Natalia Prevarskaya 1 Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, France Université des Sciences et Technologies de Lille (USTL),...
Sulfonylureas stimulate insulin secretion by blocking ATP-sensitive K+ channels (K+-ATP channels) of the beta-cell membrane, thereby causing depolarization, Ca2+ influx, and rise in cytoplasmic concentration ([Ca2+]i), whereas diazoxide inhibits opening K+-ATP channels. It has been suggested recently that these drugs also respectively increase decrease efficacy on exocytosis. This hypothesis was tested here with intact islets or single beta-cells from normal mice. Depolarizing islet cells...
BACKGROUND Very little is known about the functional expression and physiological role of ryanodine receptors in nonexcitable cells, prostate cancer cells particular. Nonetheless, different studies have demonstrated that calcium a major factor involved apoptosis. Therefore, calcium-regulatory mechanisms, such as ryanodine-mediated release, may play substantial regulation METHODS We assessed presence LNCaP using fluorimetric measurements intracellular assays mRNA encoding receptors. RESULTS...
In the present study, we investigated effect of antiestrogen compound tamoxifen on BK channels by use patch-clamp technique. The perfusion 10 nM significantly increased magnitude a voltage-dependent K<sup>+</sup> current 22.6 ± 10.6% (<i>n</i> = 23). was always obtained in first minute, peaked at 5.9 2.2 min 23), and abolished tetraethylammonium (0.5 mM), charybdotoxin (50 nM), or iberiotoxin (100 nM). stimulatory same low nM) high (700 internal calcium concentration not additive to that...