A5000158090- Clinical Nutrition and Gastroenterology
- Pediatric Hepatobiliary Diseases and Treatments
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Maternal and Perinatal Health Interventions
- Bacterial Genetics and Biotechnology
- Retinoids in leukemia and cellular processes
- Chemical Reactions and Isotopes
- Nuclear Receptors and Signaling
- Nutrition, Genetics, and Disease
- Digestive system and related health
- CRISPR and Genetic Engineering
- Kruppel-like factors research
- Dermatological and COVID-19 studies
- Respiratory viral infections research
- Genomics and Chromatin Dynamics
- Parvovirus B19 Infection Studies
- Histone Deacetylase Inhibitors Research
- Genomics, phytochemicals, and oxidative stress
- Geotourism and Geoheritage Conservation
- Groundwater and Watershed Analysis
- Folate and B Vitamins Research
- Phytoestrogen effects and research
- Infant Nutrition and Health
- Child Nutrition and Feeding Issues
Worcestershire Acute Hospitals NHS Trust
2024
University of Colorado Denver
2016-2023
Children's Hospital Colorado
2021-2023
Colorado School of Public Health
2023
Girls Incorporated
2023
University of Colorado Anschutz Medical Campus
2016-2021
University of Calcutta
1969-2019
National Institutes of Health
2011
National Institute of Cholera and Enteric Diseases
2010-2011
Post Graduate Institute of Medical Education and Research
2010
Abstract In infants intolerant of enteral feeding because intestinal disease, parenteral nutrition may be associated with cholestasis, which can progress to end-stage liver disease. Here we show the function hepatic macrophages and phytosterols in nutrition-associated cholestasis (PNAC) pathogenesis using a mouse model that recapitulates human pathophysiology combines injury nutrition. We combine genetic, molecular, pharmacological approaches identify an essential IL-1β PNAC. Pharmacological...
The TET family of FE(II) and 2-oxoglutarate-dependent enzymes (Tet1/2/3) promote DNA demethylation by converting 5-methylcytosine to 5-hydroxymethylcytosine (5hmC), which they further oxidize into 5-formylcytosine 5-carboxylcytosine. Tet1 is robustly expressed in mouse embryonic stem cells (mESCs) has been implicated mESC maintenance. Here we demonstrate that, unlike genetic deletion, RNAi-mediated depletion mESCs led a significant reduction 5hmC loss identity. differentiation phenotype due...
Objectives : To identify the etiology and pregnancy outcome of hydrops fetalis in a cohort pregnancies referred to tertiary maternal fetal medicine center UK. These data allow review large series affected by emphasize importance investigation then treatment individual cases. This provides parents with improved information especially specific prognostic information. Methods A retrospective 63 consecutive cases managed between September 1996 March 1999. Results Of pregnancies, 12.7% ( n = 8)...
To identify the etiology and pregnancy outcome of hydrops fetalis in a cohort pregnancies referred to tertiary maternal fetal medicine center UK. These data allow review large series affected by emphasize importance investigation then treatment individual cases. This provides parents with improved information especially specific prognostic information.A retrospective 63 consecutive cases managed between September 1996 March 1999.Of pregnancies, 12.7% (n = 8) were associated an 'immune'...
Abstract Background and Aims Parenteral nutrition (PN)–associated cholestasis (PNAC) complicates the care of patients with intestinal failure. In PNAC, phytosterol containing PN synergizes injury IL‐1β derived from activated hepatic macrophages to suppress hepatocyte farnesoid X receptor (FXR) signaling promote PNAC. We hypothesized that pharmacological activation FXR would prevent PNAC in a mouse model. Approach Results To induce male C57BL/6 mice were subjected (2% dextran sulfate sodium...
Significance A key step to understanding a phenotype of interest is the identification genes defining that phenotype. We propose computational framework for systematic integration published gene expression data identify cell identity interest. demonstrate utility proposed approach by identifying essential maintenance ES (ESC) identity. Follow-up functional studies on candidate Nucleolin ( Ncl ) reveal 's role in ESC homeostasis. deficiency increases endogenous reactive oxygen species levels...
Human metapneumovirus (hMPV) is associated with the acute respiratory tract infection (ARTI) in all age groups. However, there limited information on prevalence and genetic diversity of human strains circulating India.To study genomic hMPV among ARTI patients reporting outpatient departments hospitals Kolkata, Eastern India.Nasal and/or throat swabs from 2309 during January 2006 to December 2009, were screened for presence by RT-PCR nucleocapsid (N) gene. The G F genes representative...
Background and Aims Chronically administered parenteral nutrition (PN) in patients with intestinal failure carries the risk for developing PN-associated cholestasis (PNAC). We have demonstrated that farnesoid X receptor (FXR) liver (LXR), proinflammatory interleukin-1 beta (IL-1β), infused phytosterols are important murine PNAC pathogenesis. In this study we examined role of nuclear homolog 1 (LRH-1) PNAC. Approach Results a C57BL/6 mouse model (dextran sulfate sodium [DSS] pretreatment...
Abstract Growth hormone (GH) is one of the critical factors in maintaining glucose metabolism. B-cell translocation gene 2 (BTG2) and yin yang 1 (YY1) are key regulators diverse metabolic processes. In this study, we investigated link between GH BTG2–YY1 signaling pathway treatment elevated expression hepatic Btg2 Yy1 primary mouse hepatocytes livers. Glucose production serum blood levels were increased during exposure. Overexpression induced gluconeogenic enzymes phosphoenolpyruvate...
The 50 S ribosome of Escherichia coli is partially degraded by RNase I in presence a high concentration Mg2+ (10 to 20 mM); the subunit becomes resistant further action I. latter remains latent association with subparticle as case 30 (Neu, H.C., and Heppel, L.A. (1954) Proc. Natl. Acad. Sci. U.S.A. 51, 1267-1274). As result nucleolytic action, 23 RNA smaller size four proteins (L4, L10, L7/L12) are released from subunit. From location these proteins, it appears that primary site central...
Abstract After the interaction of estrogen with ligand binding domain (LBD) mouse receptor‐α (mERα) and hormone‐responsive elements target genes, many nuclear proteins are recruited to regulate expression specific genes. Because it is not known which brain interact LBD or whether these vary age sex, we used pull‐down assay far Western blotting detect five 160, 140, 87, 60, 46 kD in brain. These interacting were identified as PELP1, RIP140, PGC1α, BAF60, ADA3, respectively. The level BAF60...
Abstract Estrogen receptor (ER)‐α interacts with nuclear proteins to mediate its multiple functions in the brain. However, it is not known which interact ERα‐transactivation domain (TAD) mouse brain and whether they change age sex. Therefore, we have used affinity‐purified GST‐tagged ERα‐TAD fusion protein for interaction from The pull‐down assay far‐Western blotting detected four of 100, 80, 68, 50 kD. We recently identified 80‐kD as MTA1 demonstrated decrease old age. Here report...
Background and Aims: Parenteral nutrition (PN) in patients with intestinal failure can lead to cholestasis (PNAC). In a PNAC mouse model, farnesoid X receptor (FXR) agonist (GW4064) treatment alleviated IL-1β-dependent cholestatic liver injury. The objective of this study was determine whether hepatic protection FXR activation is mediated through IL-6-STAT3 signaling. Approach Results: Hepatic apoptotic pathways [Fas-associated protein death domain ( Fas ) mRNA, caspase 8 protein, cleaved 3]...
Abstract Scientists and researchers in ancient modern times have profoundly applied morphometric analysis to evaluate quantitative description of landforms or drainage basins large regions. The objective this study is enlighten certain features like tectonic control over basin, the hydro-geomorphic characteristics system geomorphic maturity terrain South Andaman Island. After extensive studies, particular island broadly classified into five major patterns (dendritic, trellis, parallel,...
Other SectionsABSTRACTINTRODUCTIONRESULTSDISCUSSIONMATERIALS AND METHODSSupplementary MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTFIGURESREFERENCES
Key content: Sarcoidosis is an uncommon multi‐system disorder characterised by the presence of non‐caseating granulomas. It has a peak incidence between ages 20–40 years old. The pathogenesis sarcoidosis uncertain; however, it known to be associated with exaggerated T helper 1 (T H 1) immune response leading systemic inflammation and granuloma formation. Suppression in responses pregnancy leads disease remission majority pregnancies. Nevertheless, potential for decompensation subgroup...
Abstract Prolonged parenteral nutrition (PN) can lead to PN associated cholestasis (PNAC). Intestinally derived lipopolysaccharides and infused phytosterols activation of NFκB, a key factor in PNAC. Our objective was determine if inhibition HNF4α could interfere with NFκB alleviate murine We showed that antagonist BI6015 (20 mg/kg/day) DSS-PN (oral DSS x4d followed by Total x14d) mice prevented the increased AST, ALT, bilirubin bile acids reversed mRNA suppression hepatocyte Abcg5/8, Abcb11,...
Background and Aims: Parenteral nutrition-associated cholestasis (PNAC) is an important complication in patients with intestinal failure reduced LRH-1 expression. Here, we hypothesized that activation by its agonist, dilauroylphosphatidylcholine (DLPC), would trigger signal transducer activator of transcription 6 (STAT6) signaling hepatic macrophage polarization mediate protection PNAC. Approach Results: PNAC mouse model (oral DSSx4d followed PNx14d; DSS-PN) was treated agonist DLPC (30...