The propagation of coherent light in a dynamic disordered medium generates speckle patterns. dynamics intrinsic to the result shorter decorrelation time for multiple scattering component (τcM) relative single (τcS). In this study, we propose single-frame statistical gating strategy and components reflective wide-field imaging when exposure T satisfies condition τcM<T<τcS. spatial matrix representation is modeled as sum negative exponential matrix, which represents component,...

We designed a miniature laser speckle imager that weighs ∼20 g and is 3.1-cm high for full-field high-resolution imaging of cerebral blood flow (CBF) in freely moving animals. Coherent light illuminates the cortex through multimode optical fiber bundle fixed onto supporting frame imager. The reflected lights are then collected by macrolens system imaged CMOS camera at rate (50 fps). Using this imager, we achieve spatiotemporal resolution contrast CBF animals real time.

The detection of deep-seated lesions is great significance for biomedical applications. However, due to the strong photon absorption and scattering biological tissues, it challenging realize in vivo deep optical detections, particularly those using safe laser irradiance below clinical maximum permissible exposure (MPE). In this work, combination ultra-bright surface-enhanced Raman (SERS) nanotags transmission spectroscopy (TRS) reported achieve non-invasive photosafe "phantom" deeply hidden...

Laser speckle imaging (LSI) has been widely used for in vivo detecting cerebral blood flow (CBF) under various physiological and pathological conditions. So far, nearly all literature on LSI does not consider the influence of disturbances due to respiration and/or heart beating animals. In this paper, we analyze how such physiologic motions affect spatial resolution conventional laser contrast analysis (LASCA). We propose a registered (rLASCA) method which first registers raw images with 3 x...

Laboratory and clinical studies have demonstrated that therapeutic hypothermia (TH), when applied as soon possible after resuscitation from cardiac arrest (CA), results in better neurological outcome. This study tested the hypothesis TH would promote cerebral blood flow (CBF) restoration its maintenance return of spontaneous circulation (ROSC) CA. Twelve Wistar rats resuscitated 7-min asphyxial CA were randomized into two groups: group (7 H, n = 6), treated with mild (33–34℃) immediately...

Transient middle cerebral artery occlusion (tMCAO) model is widely used to mimic human focal ischemic stroke in order study ischemia/reperfusion brain injury rodents. In tMCAO model, intraluminal suture technique achieve ischemia and reperfusion. However, variation of infarct volume this often requires large sample size, which hinders the progress preclinical research. Our previous demonstrated that was related success reperfusion although reason remained unclear. The aim present explore...

In this paper, we develop a random process theory to explain the laser speckle phenomena.The relation between probability distribution of speckle"s integrated intensity () t Y and relative velocity vt is derived.Based on theory, traditional spatial or temporal contrast analysis (i.e.spatial LASCA) can be derived as estimators respectively.Both LASCA suffer from noise due insufficient statistics nonstationarity in either domain.Furthermore, results reduction resolution.A new estimator...

Aim To assess the potential relationship between intelligence structure abnormalities and whole-brain functional connectivity in children with primary nocturnal enuresis (PNE) resting-state magnetic resonance imaging (fMRI) to provide insights into association these two seemingly unrelated conditions. Methods Intelligence testing fMRI data were obtained from 133 right-handed children, including 67 PNE (M/F, 39∶28; age, 10.5±1.2 y) 66 age-matched healthy controls 37∶29; 10.1±1.1 y). All tests...

Random matrix theory provides new insights into multiple scattering in random media. In a recent study, we demonstrated the statistical separation of single- and multiple-scattering components based on Wishart matrix. The first- second-order moments were estimated with constructed using dynamically backscattered speckle images. this strategy was applied to laser contrast imaging (LSCI)

Laser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels vivo, but its applications are limited by shallow penetration depth under reflective configuration. The traditional LSCI setup has been used transmissive extension up to [Formula: see text]–[Formula: text] ([Formula: the transport mean free path), estimation biased due uncertainty large of field (DOF) images. In this study, we propose multifocal depth-resolved thick tissue, further...

Chronically monitoring cerebral activities in awake and freely moving status is very important physiological pathological studies.We present a novel standalone micro-imager for the blood flow (CBF) total hemoglobin (HbT) animals using laser speckle contrast imaging (LSCI) optical intrinsic signal (OIS) methods.A new cranial window method, contact lens wide field optics, also proposed to achieve chronic wide-field of rat's cortex.The hemodynamic rats' cortex were measured first time without...

Laser speckle contrast analysis (LASCA) has been demonstrated as a full-field method for imaging the cerebral blood flow (CBF). However, conventional LASCA is limited to extremely low dynamic range because of ambient background field, dark current, and other anomalies in circuits charge-coupled device camera, which makes it difficult analyze spatiotemporal variabilities CBF. In this study, we proposed an enhanced (eLASCA) improve based on monotonic point transformation. investigating...

Induced hypothermia has been broadly applied in neurological intensive care unit (NICU). Meanwhile, accidental is also a threatening condition daily life. It meaningful to investigate the influences of temperature change on cerebral blood flow (CBF). In present study, temporal laser speckle image contrast analysis (tLASCA) was implemented study relative CBF artery, vein and capillary level under mild (35degC) moderate (32degC) hypothermia. Twelve male Sprague-Dawley rats (300 plusmn 50 g)...

The wave propagation in a random medium plays critical role optics and quantum physics. Multiple scattering of coherent determines the transport procedure. Brownian motions scatterers perturb each trajectory form dynamic speckle patterns backscattered direction. In this study, we applied matrix theory to investigate eigenvalue density intensity matrix. We find that can be utilized decouple single multiple components. Wishart component is well described by Marčenko–Pastur law, while part has...

Dynamic laser speckles contain motion information of scattering particles which can be estimated by speckle contrast analysis (LASCA). In this work, an entropy-based method was proposed to provide a more robust estimation speed. An in vitro flow simulation experiment confirmed simple linear relation between entropy, exposure time, and A multimodality optical imaging setup is developed validate the advantages entropy based on imaging, green light fluorescence imaging. The overcomes...

Hypothermia can unintentionally occur in daily life, e.g., cardiovascular surgery or applied as therapeutics the neurosciences critical care unit. So far, temperature-induced spatiotemporal responses of neural function have not been fully understood. In this study, we investigated functional change cerebral blood flow (CBF), accompanied with neuronal activation, by laser speckle imaging (LSI) during hypothermia. Laser images from Sprague-Dawley rats (n = 8, male) were acquired under...

Imaging brain microvasculature is important in plasticity studies of cerebrovascular diseases. Applying contrast agents, traditional μ CT and MRI methods gain imaging for vasculature. The aim this study to develop a synchrotron radiation X-ray inline phase-contrast tomography (SRXPCT) method the intact mouse (micro)vasculature high resolution (~3.7 m) without agent. A specific preparation protocol was proposed enhance phase vasculature by using density difference over gas-tissue interface....

Abstract A series of studies have revealed that nocturnal enuresis is closely related to hypoxia in children with primary (PNE). However, brain oxygen metabolism PNE has not been investigated before. The purpose this study was investigate changes whole‐brain cerebral metabolic rate (CMRO 2 ), blood flow (CBF), and extraction fraction (OEF) suffering from PNE. We used the newly developed T2‐relaxation‐under‐spin‐tagging (TRUST) magnetic resonance imaging technique. Neurological evaluation,...

Laser speckle contrast imaging (LSCI) has been widely used to determine blood flow and perfusion in biological tissues. The physical model of traditional LSCI ignores the effects scattering property distribution relation correlation time τc v, which further results biased estimation. In this study, we developed a dual-wavelength laser (dwLSCI) method portable device for tissue human hands. Experimental data showed that dwLSCI could retrieve vein vasculatures under surface skin, it provided...